ID   UCRI_BRAJA              Reviewed;         176 AA.
AC   P51130;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-JUN-2009, entry version 66.
DE   RecName: Full=Ubiquinol-cytochrome c reductase iron-sulfur subunit;
DE            EC=1.10.2.2;
DE   AltName: Full=Rieske iron-sulfur protein;
DE            Short=RISP;
GN   Name=petA; Synonyms=fbcF; OrderedLocusNames=blr2485;
OS   Bradyrhizobium japonicum.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=375;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=USDA 110;
RX   MEDLINE=89249332; PubMed=2541921; DOI=10.1016/0092-8674(89)90137-2;
RA   Thoeny-Meyer L., Stax D., Hennecke H.;
RT   "An unusual gene cluster for the cytochrome bc1 complex in
RT   Bradyrhizobium japonicum and its requirement for effective root nodule
RT   symbiosis.";
RL   Cell 57:683-697(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=USDA 110;
RX   MEDLINE=22484998; PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T.,
RA   Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K.,
RA   Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M.,
RA   Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Component of the ubiquinol-cytochrome c reductase
CC       complex (complex III or cytochrome b-c1 complex), which is a
CC       respiratory chain that generates an electrochemical potential
CC       coupled to ATP synthesis.
CC   -!- CATALYTIC ACTIVITY: QH(2) + 2 ferricytochrome c = Q + 2
CC       ferrocytochrome c + 2 H(+).
CC   -!- COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
CC   -!- SUBUNIT: The main subunits of complex b-c1 are: cytochrome b,
CC       cytochrome c1 and the Rieske protein.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Single-pass membrane protein.
CC   -!- DEVELOPMENTAL STAGE: Required for nitrogen fixation in root
CC       nodules on soybeans, but not for aerobic growth. It seems however
CC       to be expressed under many growth conditions (aerobic,
CC       microaerobic and in nodule bacteroids).
CC   -!- MISCELLANEOUS: The Rieske protein is a high potential 2Fe-2S
CC       protein.
CC   -!- SIMILARITY: Contains 1 Rieske domain.
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DR   EMBL; J03176; AAA26199.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC47750.1; -; Genomic_DNA.
DR   PIR; A32382; A32382.
DR   RefSeq; NP_769125.1; -.
DR   HSSP; P13272; 1RIE.
DR   GeneID; 1050391; -.
DR   GenomeReviews; BA000040_GR; blr2485.
DR   KEGG; bja:blr2485; -.
DR   NMPDR; fig|224911.1.peg.2485; -.
DR   HOGENOM; P51130; -.
DR   OMA; P51130; VVGICSH.
DR   BioCyc; BJAP224911:BLR2485-MON; -.
DR   BRENDA; 1.10.2.2; 280.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008121; F:ubiquinol-cytochrome-c reductase activity; IEA:EC.
DR   GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
DR   GO; GO:0006810; P:transport; IEA:UniProtKB-KW.
DR   InterPro; IPR017941; Rieske_2Fe-2S.
DR   InterPro; IPR014349; Rieske_Fe-S_prot.
DR   InterPro; IPR006311; Tat.
DR   InterPro; IPR017909; Twin_arg_translocation_Tat.
DR   InterPro; IPR019470; Ubiq_cytC_Rdtase_Fe-S_su_TAT.
DR   InterPro; IPR006317; Ubiquinol_cyt_c_Rdtase_Fe-S-su.
DR   Gene3D; G3DSA:2.102.10.10; Rieske_reg; 1.
DR   PANTHER; PTHR10134; Rieske; 1.
DR   Pfam; PF00355; Rieske; 1.
DR   Pfam; PF10399; UCR_Fe-S_N; 1.
DR   TIGRFAMs; TIGR01416; Rieske_proteo; 1.
DR   TIGRFAMs; TIGR01409; TAT_signal_seq; 1.
DR   PROSITE; PS51296; RIESKE; 1.
DR   PROSITE; PS51318; TAT; 1.
PE   2: Evidence at transcript level;
KW   2Fe-2S; Cell membrane; Complete proteome; Disulfide bond;
KW   Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding;
KW   Oxidoreductase; Transmembrane; Transport.
FT   CHAIN         1    176       Ubiquinol-cytochrome c reductase iron-
FT                                sulfur subunit.
FT                                /FTId=PRO_0000127759.
FT   TRANSMEM     15     36       Potential.
FT   DOMAIN       87    174       Rieske.
FT   METAL       119    119       Iron-sulfur (2Fe-2S) (By similarity).
FT   METAL       121    121       Iron-sulfur (2Fe-2S); via pros nitrogen
FT                                (By similarity).
FT   METAL       138    138       Iron-sulfur (2Fe-2S) (By similarity).
FT   METAL       141    141       Iron-sulfur (2Fe-2S); via pros nitrogen
FT                                (By similarity).
FT   DISULFID    124    140       By similarity.
SQ   SEQUENCE   176 AA;  18823 MW;  19C636F520E3FBF5 CRC64;
     MTTASSADHP TRRDFLFVAT GAAAAVGGAA ALWPFISQMN PDASTIAAGA PIEVDLSPIA
     EGQDIKVFWR GKPIYISHRT KKQIDEARAV NVASLPDPQS DEARVKSGHE QWLVVIGICT
     HLGCIPIAHE GNYDGFFCPC HGSQYDSSGR IRQGPAPANL PVPPYQFVSD TKIQIG
//