Reviewed, UniProtKB/Swiss-Prot P51130 (UCRI_BRAJA)

Names and origin

Protein names

Recommended name:
    Ubiquinol-cytochrome c reductase iron-sulfur subunit
    EC=1.10.2.2
Alternative name(s):
    Rieske iron-sulfur protein
      Short name=RISP

Gene names

Name:	petA
Synonyms:	fbcF
Ordered Locus Names:	blr2485

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	176 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
Catalytic activity	QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein.
Subcellular location	Cell membrane; Single-pass membrane protein.
Developmental stage	Required for nitrogen fixation in root nodules on soybeans, but not for aerobic growth. It seems however to be expressed under many growth conditions (aerobic, microaerobic and in nodule bacteroids).
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 176	176	Ubiquinol-cytochrome c reductase iron-sulfur subunit		PRO_0000127759
Regions
	Transmembrane	15 – 36	22	Potential
	Domain	87 – 174	88	Rieske
Sites
	Metal binding	119	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	121	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	138	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	141	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	124 ↔ 140		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51130-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 19C636F520E3FBF5	FASTA	176	18,823
	10 20 30 40 50 60 MTTASSADHP TRRDFLFVAT GAAAAVGGAA ALWPFISQMN PDASTIAAGA PIEVDLSPIA 70 80 90 100 110 120 EGQDIKVFWR GKPIYISHRT KKQIDEARAV NVASLPDPQS DEARVKSGHE QWLVVIGICT 130 140 150 160 170 HLGCIPIAHE GNYDGFFCPC HGSQYDSSGR IRQGPAPANL PVPPYQFVSD TKIQIG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An unusual gene cluster for the cytochrome bc1 complex in Bradyrhizobium japonicum and its requirement for effective root nodule symbiosis." Thoeny-Meyer L., Stax D., Hennecke H. Cell 57:683-697(1989) [PubMed: 2541921] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: USDA 110.
[2]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J03176 Genomic DNA. Translation: AAA26199.1. BA000040 Genomic DNA. Translation: BAC47750.1.
PIR	A32382.
RefSeq	NP_769125.1.
3D structure databases
SMR	P51130. Positions 10-176.
ModBase	Search...
Genome annotation databases
GeneID	1050391.
GenomeReviews	Gene locus blr2485 in contig BA000040_GR.
KEGG	bja:blr2485.
NMPDR	fig|224911.1.peg.2485.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG668715.
OMA	GICSHLG.
PhylomeDB	P51130.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR2485-MONOMER.
BRENDA	1.10.2.2. 280.
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. IPR019470. Ubiq_cytC_Rdtase_Fe-S_su_TAT. IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF10399. UCR_Fe-S_N. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
TIGRFAMs	TIGR01416. Rieske_proteo. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_BRAJA

Accession

Primary (citable) accession number: P51130

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)