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Protein

Calpastatin

Gene

Cast

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Protein family/group databases

MEROPSiI27.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpastatin
Alternative name(s):
Calpain inhibitor
Gene namesi
Name:Cast
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1098236. Cast.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • endoplasmic reticulum Source: MGI
  • membrane Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 788788CalpastatinPRO_0000147633Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111PhosphoserineCombined sources
Cross-linki112 – 112Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei129 – 1291N6-acetyllysineCombined sources
Modified residuei165 – 1651PhosphoserineCombined sources
Modified residuei216 – 2161PhosphothreonineBy similarity
Modified residuei219 – 2191PhosphoserineCombined sources
Modified residuei303 – 3031PhosphoserineCombined sources
Modified residuei324 – 3241PhosphoserineBy similarity
Modified residuei444 – 4441PhosphoserineBy similarity
Modified residuei446 – 4461PhosphoserineBy similarity
Modified residuei453 – 4531PhosphoserineBy similarity
Modified residuei479 – 4791PhosphothreonineCombined sources
Modified residuei518 – 5181PhosphoserineBy similarity
Modified residuei594 – 5941PhosphoserineBy similarity
Modified residuei605 – 6051PhosphoserineBy similarity
Modified residuei653 – 6531PhosphoserineBy similarity
Modified residuei655 – 6551PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP51125.
MaxQBiP51125.
PaxDbiP51125.
PRIDEiP51125.

PTM databases

iPTMnetiP51125.
PhosphoSiteiP51125.

Expressioni

Tissue specificityi

Isoform 2 is the major form in all tissues examined. Isoform 1 accounts for 5-10% in tissues such as skeletal muscle, liver and brain, and 30% in myoblasts. Isoforms 4 and 5 are testis-specific. Isoform 6 is highly expressed in heart and skeletal muscle with lower levels in liver, brain and testis. Isoform 7 is expressed at high levels in liver.2 Publications

Gene expression databases

CleanExiMM_CAST.

Interactioni

Protein-protein interaction databases

IntActiP51125. 3 interactions.
MINTiMINT-4098325.
STRINGi10090.ENSMUSP00000065275.

Structurei

3D structure databases

ProteinModelPortaliP51125.
SMRiP51125. Positions 237-321, 644-734.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati251 – 30353Inhibitory domain 1Add
BLAST
Repeati384 – 43653Inhibitory domain 2Add
BLAST
Repeati524 – 57754Inhibitory domain 3Add
BLAST
Repeati661 – 71454Inhibitory domain 4Add
BLAST

Domaini

Each of the four flexible inhibitory domains can inhibit one calcium-bound calpain molecule by occupying both sides of the active site.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IFF9. Eukaryota.
ENOG4111YF0. LUCA.
HOGENOMiHOG000082405.
HOVERGENiHBG000183.
InParanoidiP51125.
KOiK04281.
PhylomeDBiP51125.

Family and domain databases

InterProiIPR026998. Calpastatin.
IPR001259. Prot_inh_calpain.
[Graphical view]
PANTHERiPTHR10077. PTHR10077. 1 hit.
PfamiPF00748. Calpain_inhib. 4 hits.
[Graphical view]

Sequences (7)i

Sequence statusi: Complete.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P51125-1) [UniParc]FASTAAdd to basket

Also known as: MCS-A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQPGPKPAA SPRPSRGAAA RHTQEHVNEK NIGSSSKPGE KKGSDEKKAA
60 70 80 90 100
SLGSSQPSRP HVGEAATATK VTASSAATSK SPSMSTTETK AIPVNKQLEG
110 120 130 140 150
PDQKRPREQA VKTESKKPQS SEQPVVHEKK SKGGPKEGSE PKNLPKHTSS
160 170 180 190 200
TGSKHAHKEK ALSRSNEQMV SEKPSESKTK FQDVPSAGGE SVAGGGTVAT
210 220 230 240 250
ALDKVVGKKK EQKPFTPASP VQSTPSKPSD KSGMDAALDD LIDTLGGHED
260 270 280 290 300
TNRDDPPYTG PVVLDPMYST YLEALGIKEG TIPPEYRKLL EKNEGITQPL
310 320 330 340 350
PDSPKPMGTD QAIDALSSDF TCSSPTGKQS EKEKSTGEIF KAQSAGVTRS
360 370 380 390 400
SVPPKEKKRK VEEEVINDQA LQALSDSLGT RQPDPPSHVS QAEQVKEAKA
410 420 430 440 450
KEERQEKCGE DEDTVPAEYR LKPAKDKDGK PLLPEPEETS KSLSESELIG
460 470 480 490 500
ELSADFDRST YQDKPSTPAE KKSNDTSQTP PGETVPRASM CSIRSAPPKL
510 520 530 540 550
ASLKGVVPED AVETLAGSLG TREADPEHEK TVEDKVKEKA KEEEHEKLGE
560 570 580 590 600
KEETVPPDYR LEEVKDKDGK PLLPKESQEQ LAPLSDDFLL DALSQDFSSP
610 620 630 640 650
ANISSLEFED AKLSAAISEV VSQTPAPSTH AAAPLPGTEQ KDKELDDALD
660 670 680 690 700
ELSDSLGQRP PDPDENKPLD DKVKEKIKPE HSEKLGERDD TIPPEYRHLL
710 720 730 740 750
DNDGKDKPEK PPTKKTEKPD QDRDPIDALS EDLDSCPSTT ETSKNTAKGK
760 770 780
SKKTSSSKAS KDGEKTKDSS KKTEEVSKPK AKEDARHS
Length:788
Mass (Da):84,922
Last modified:August 13, 2002 - v2
Checksum:i28E4D3B4A68BFEB9
GO
Isoform 2 (identifier: P51125-2) [UniParc]FASTAAdd to basket

Also known as: MCS-B

The sequence of this isoform differs from the canonical sequence as follows:
     92-110: Missing.

Show »
Length:769
Mass (Da):82,736
Checksum:i7A50CE154510369F
GO
Isoform 3 (identifier: P51125-3) [UniParc]FASTAAdd to basket

Also known as: MCS-C

The sequence of this isoform differs from the canonical sequence as follows:
     92-110: Missing.
     305-333: Missing.

Show »
Length:740
Mass (Da):79,695
Checksum:i09DD44D777D45F82
GO
Isoform 4 (identifier: P51125-4) [UniParc]FASTAAdd to basket

Also known as: TCAST1

The sequence of this isoform differs from the canonical sequence as follows:
     1-341: Missing.
     342-396: AQSAGVTRSS...SHVSQAEQVK → MGQFLSSTFW...EEREHGSKHH

Show »
Length:446
Mass (Da):49,125
Checksum:iA2C57CE4FD7E04FC
GO
Isoform 5 (identifier: P51125-5) [UniParc]FASTAAdd to basket

Also known as: TCAST2

The sequence of this isoform differs from the canonical sequence as follows:
     1-357: Missing.
     358-397: KRKVEEEVIN...HVSQAEQVKE → MGQFLSSTFW...GETIPILQDH

Show »
Length:431
Mass (Da):47,366
Checksum:i38C6B615B38171C2
GO
Isoform 6 (identifier: P51125-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: MSQPGPKPAASPRPSRGAAARHTQE → MAFASWWYKT

Show »
Length:773
Mass (Da):83,626
Checksum:i7CDE4420D3AC052B
GO
Isoform 7 (identifier: P51125-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-83: Missing.

Show »
Length:705
Mass (Da):76,608
Checksum:i4B65B35982DFC983
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 357357Missing in isoform 5. CuratedVSP_000749Add
BLAST
Alternative sequencei1 – 341341Missing in isoform 4. CuratedVSP_000747Add
BLAST
Alternative sequencei1 – 8383Missing in isoform 7. CuratedVSP_000746Add
BLAST
Alternative sequencei1 – 2525MSQPG…RHTQE → MAFASWWYKT in isoform 6. CuratedVSP_000745Add
BLAST
Alternative sequencei92 – 11019Missing in isoform 2 and isoform 3. 1 PublicationVSP_000751Add
BLAST
Alternative sequencei305 – 33329Missing in isoform 3. 1 PublicationVSP_000752Add
BLAST
Alternative sequencei342 – 39655AQSAG…AEQVK → MGQFLSSTFWEGSPAAVWQE KLREGERKGAGETIPILQDH VICSEEREHGSKHH in isoform 4. CuratedVSP_000748Add
BLAST
Alternative sequencei358 – 39740KRKVE…EQVKE → MGQFLSSTFWEGSPAAVWQE KLREGERKGAGETIPILQDH in isoform 5. CuratedVSP_000750Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026997 mRNA. Translation: BAA84768.1.
AF190152 mRNA. Translation: AAF25194.1.
AF190151 mRNA. Translation: AAF25193.1.
AB044334 Genomic DNA. Translation: BAB18888.1.
AB044334 Genomic DNA. Translation: BAB18889.1.
AB044334 Genomic DNA. Translation: BAB18886.1.
AB044334 Genomic DNA. Translation: BAB18887.1.
X62519 mRNA. Translation: CAA44385.1.
PIRiS20610.
RefSeqiNP_001288082.1. NM_001301153.1. [P51125-1]
NP_001288089.1. NM_001301160.1. [P51125-5]
NP_001288110.1. NM_001301181.1. [P51125-4]
XP_006517122.1. XM_006517059.1. [P51125-2]
XP_011242768.1. XM_011244466.1. [P51125-6]
XP_011242769.1. XM_011244467.1. [P51125-7]
UniGeneiMm.29163.
Mm.441995.

Genome annotation databases

GeneIDi12380.
KEGGimmu:12380.
UCSCiuc007rfl.2. mouse. [P51125-4]
uc007rfq.2. mouse. [P51125-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026997 mRNA. Translation: BAA84768.1.
AF190152 mRNA. Translation: AAF25194.1.
AF190151 mRNA. Translation: AAF25193.1.
AB044334 Genomic DNA. Translation: BAB18888.1.
AB044334 Genomic DNA. Translation: BAB18889.1.
AB044334 Genomic DNA. Translation: BAB18886.1.
AB044334 Genomic DNA. Translation: BAB18887.1.
X62519 mRNA. Translation: CAA44385.1.
PIRiS20610.
RefSeqiNP_001288082.1. NM_001301153.1. [P51125-1]
NP_001288089.1. NM_001301160.1. [P51125-5]
NP_001288110.1. NM_001301181.1. [P51125-4]
XP_006517122.1. XM_006517059.1. [P51125-2]
XP_011242768.1. XM_011244466.1. [P51125-6]
XP_011242769.1. XM_011244467.1. [P51125-7]
UniGeneiMm.29163.
Mm.441995.

3D structure databases

ProteinModelPortaliP51125.
SMRiP51125. Positions 237-321, 644-734.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP51125. 3 interactions.
MINTiMINT-4098325.
STRINGi10090.ENSMUSP00000065275.

Protein family/group databases

MEROPSiI27.001.

PTM databases

iPTMnetiP51125.
PhosphoSiteiP51125.

Proteomic databases

EPDiP51125.
MaxQBiP51125.
PaxDbiP51125.
PRIDEiP51125.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi12380.
KEGGimmu:12380.
UCSCiuc007rfl.2. mouse. [P51125-4]
uc007rfq.2. mouse. [P51125-1]

Organism-specific databases

CTDi831.
MGIiMGI:1098236. Cast.

Phylogenomic databases

eggNOGiENOG410IFF9. Eukaryota.
ENOG4111YF0. LUCA.
HOGENOMiHOG000082405.
HOVERGENiHBG000183.
InParanoidiP51125.
KOiK04281.
PhylomeDBiP51125.

Miscellaneous databases

PROiP51125.
SOURCEiSearch...

Gene expression databases

CleanExiMM_CAST.

Family and domain databases

InterProiIPR026998. Calpastatin.
IPR001259. Prot_inh_calpain.
[Graphical view]
PANTHERiPTHR10077. PTHR10077. 1 hit.
PfamiPF00748. Calpain_inhib. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of mouse calpastatin isoforms: implications of species-common and species-specific alternative splicing."
    Takano J., Kawamura T., Murase M., Hitomi K., Maki M.
    Biochem. Biophys. Res. Commun. 260:339-345(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY.
    Strain: C3H/HeJ.
    Tissue: Muscle.
  2. "Four types of calpastatin isoforms with distinct amino-terminal sequences are specified by alternative first exons and differentially expressed in mouse tissues."
    Takano J., Watanabe M., Hitomi K., Maki M.
    J. Biochem. 128:83-92(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 4; 5; 6 AND 7), TISSUE SPECIFICITY.
  3. "Characterization of a membrane associated testis-specific calpastatin."
    Li S., Goldberg E.
    Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 4 AND 5).
    Strain: CD-1.
  4. "Multiple forms of rat calpastatin cDNA in the coding region of functionally unknown amino-terminal domain."
    Lee W.J., Hatanaka M., Maki M.
    Biochim. Biophys. Acta 1129:251-253(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 84-363 (ISOFORM 2).
    Strain: BALB/cJ.
    Tissue: Heart.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-479, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-219, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11 AND THR-479, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165; SER-219; SER-303 AND THR-479, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  9. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-129, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiICAL_MOUSE
AccessioniPrimary (citable) accession number: P51125
Secondary accession number(s): Q9EQV4
, Q9EQV5, Q9QXQ3, Q9QXQ4, Q9R0N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: August 13, 2002
Last modified: March 16, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.