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Reviewed, UniProtKB/Swiss-Prot P51124 (GRAM_HUMAN)

Last modified November 25, 2008. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Granzyme M
    EC=3.4.21.-
Alternative name(s):
    Met-ase
    Natural killer cell granular protease
    HU-Met-1
    Met-1 serine protease
Gene names
Name: GZMM
Synonyms: MET1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length257 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Cleaves peptide substrates after methionine, leucine, and norleucine.

Subcellular location

Secreted. Cytoplasmic granule. Note= Granules of large granular lymphocytes.

Sequence similarities

Belongs to the peptidase S1 family. Granzyme subfamily.

Contains 1 peptidase S1 domain.

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Ontologies

Keywords

   Biological processCytolysis
   Cellular componentSecreted
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMGlycoprotein
Zymogen
   Technical term3D-structure

Gene Ontology (GO)

   Biological processcytolysis

Inferred from electronic annotation. Source: UniProtKB-KW

proteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Propeptide24 – 252Activation peptide Potential
PRO_0000027421
Chain26 – 257232Granzyme M
PRO_0000027422

Regions

Domain26 – 254229Peptidase S1

Sites

Active site661Charge relay system By similarity
Active site1111Charge relay system By similarity
Active site2071Charge relay system By similarity

Amino acid modifications

Glycosylation1771N-linked (GlcNAc...) Potential
Disulfide bond51 ↔ 67 By similarity
Disulfide bond145 ↔ 213 By similarity
Disulfide bond176 ↔ 192 By similarity
Disulfide bond203 ↔ 230 By similarity

Secondary structure

1 ......................................... 257
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P51124-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: B4E815CE58E9A801

FASTA25727,446
        10         20         30         40         50         60 
MEACVSSLLV LALGALSVGS SFGTQIIGGR EVIPHSRPYM ASLQRNGSHL CGGVLVHPKW 

        70         80         90        100        110        120 
VLTAAHCLAQ RMAQLRLVLG LHTLDSPGLT FHIKAAIQHP RYKPVPALEN DLALLQLDGK 

       130        140        150        160        170        180 
VKPSRTIRPL ALPSKRQVVA AGTRCSMAGW GLTHQGGRLS RVLRELDLQV LDTRMCNNSR 

       190        200        210        220        230        240 
FWNGSLSPSM VCLAADSKDQ APCKGDSGGP LVCGKGRVLA GVLSFSSRVC TDIFKPPVAT 

       250 
AVAPYVSWIR KVTGRSA

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References

« Hide 'large scale' references
[1]"Met-ase: cloning and distinct chromosomal location of a serine protease preferentially expressed in human natural killer cells."
Smyth M.J., Sayers T.J., Wiltrout T., Powers J.C., Trapani J.A.
J. Immunol. 151:6195-6205(1993) [PubMed: 8245461] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The human Met-ase gene (GZMM): structure, sequence, and close physical linkage to the serine protease gene cluster on 19p13.3."
Pilat D., Fink T.M., Obermaier-Skrobanek B., Zimmer M., Wekerle H., Lichter P., Jenne D.E.
Genomics 24:445-450(1994) [PubMed: 7713495] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pancreas and Spleen.
+Additional computationally mapped references.

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Cross-references

Sequence databases

L23134 mRNA. Translation: AAA59582.1.
L36922 Genomic DNA. Translation: AAA57262.1.
L36936 Genomic DNA. Translation: AAA57257.1.
BC025701 mRNA. Translation: AAH25701.1.
PIRA55634.
RefSeqNP_005308.1.
UniGeneHs.465511

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1LZPmodel-A26-257[»]
ModBaseSearch...

Protein family/group databases

MEROPSS01.139.

Genome annotation databases

EnsemblENSG00000197540. Homo sapiens. [Contig view]
GeneID3004.
KEGGhsa:3004.

Organism-specific databases

H-InvDBHIX0014555.
HGNCHGNC:4712. GZMM.
MIM600311. gene.
PharmGKBPA29090.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMP51124.
HOVERGENP51124.

Gene expression databases

ArrayExpressP51124.
CleanExHS_GZMM.
GermOnlineENSG00000197540. Homo sapiens.

Family and domain databases

InterProIPR001254. Peptidase_S1_S6.
IPR001314. Peptidase_S1A.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio11912.
SOURCESearch...

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Entry information

Entry nameGRAM_HUMAN
AccessionPrimary (citable) accession number: P51124
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 25, 2008
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents