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Protein

Granzyme M

Gene

GZMM

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha-tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei66Charge relay system1 Publication1
Active sitei111Charge relay system1 Publication1
Active sitei207Charge relay system1 Publication1

GO - Molecular functioni

  • serine-type endopeptidase activity Source: UniProtKB
  • serine-type peptidase activity Source: ProtInc

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cell death Source: UniProtKB
  • cytolysis Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Apoptosis, Cytolysis, Immunity, Innate immunity

Enzyme and pathway databases

BioCyciZFISH:ENSG00000099805-MONOMER.
BRENDAi3.4.21.78. 2681.
3.4.21.B2. 2681.
ReactomeiR-HSA-173736. Alternative complement activation.

Protein family/group databases

MEROPSiS01.139.

Names & Taxonomyi

Protein namesi
Recommended name:
Granzyme M (EC:3.4.21.-)
Alternative name(s):
Met-1 serine protease
Short name:
Hu-Met-1
Met-ase
Natural killer cell granular protease
Gene namesi
Name:GZMM
Synonyms:MET1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:4712. GZMM.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB-SubCell
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi3004.
OpenTargetsiENSG00000197540.
PharmGKBiPA29090.

Polymorphism and mutation databases

BioMutaiGZMM.
DMDMi296434527.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
PropeptideiPRO_000002742124 – 25Activation peptideSequence analysis2
ChainiPRO_000002742226 – 257Granzyme MAdd BLAST232

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi51 ↔ 67PROSITE-ProRule annotation1 Publication
Disulfide bondi145 ↔ 213PROSITE-ProRule annotation1 Publication
Disulfide bondi176 ↔ 192PROSITE-ProRule annotation1 Publication
Glycosylationi177N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi203 ↔ 230PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP51124.
PeptideAtlasiP51124.
PRIDEiP51124.

PTM databases

iPTMnetiP51124.
PhosphoSitePlusiP51124.

Expressioni

Tissue specificityi

Highly and constitutively expressed in activated natural killer (NK) cells.1 Publication

Gene expression databases

BgeeiENSG00000197540.
CleanExiHS_GZMM.
ExpressionAtlasiP51124. baseline and differential.
GenevisibleiP51124. HS.

Organism-specific databases

HPAiHPA015624.

Interactioni

Protein-protein interaction databases

BioGridi109259. 5 interactors.
IntActiP51124. 1 interactor.
STRINGi9606.ENSP00000264553.

Structurei

Secondary structure

1257
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi40 – 45Combined sources6
Beta strandi48 – 57Combined sources10
Beta strandi60 – 63Combined sources4
Helixi65 – 68Combined sources4
Helixi72 – 74Combined sources3
Beta strandi75 – 80Combined sources6
Beta strandi82 – 86Combined sources5
Beta strandi89 – 98Combined sources10
Turni105 – 107Combined sources3
Beta strandi113 – 119Combined sources7
Beta strandi144 – 149Combined sources6
Beta strandi152 – 154Combined sources3
Beta strandi164 – 170Combined sources7
Helixi173 – 176Combined sources4
Turni179 – 184Combined sources6
Beta strandi190 – 194Combined sources5
Beta strandi210 – 213Combined sources4
Turni214 – 217Combined sources4
Beta strandi218 – 223Combined sources6
Beta strandi238 – 242Combined sources5
Helixi243 – 245Combined sources3
Helixi246 – 253Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LZPmodel-A26-257[»]
2ZGCX-ray1.96A26-257[»]
2ZGHX-ray2.17A26-257[»]
2ZGJX-ray2.30A26-257[»]
2ZKSX-ray2.70A26-257[»]
ProteinModelPortaliP51124.
SMRiP51124.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP51124.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 254Peptidase S1PROSITE-ProRule annotationAdd BLAST229

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP51124.
KOiK08649.
OMAiKDQAPCK.
OrthoDBiEOG091G0HUQ.
PhylomeDBiP51124.
TreeFamiTF335738.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR033040. GZMM.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PANTHERiPTHR24256:SF132. PTHR24256:SF132. 1 hit.
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51124-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEACVSSLLV LALGALSVGS SFGTQIIGGR EVIPHSRPYM ASLQRNGSHL
60 70 80 90 100
CGGVLVHPKW VLTAAHCLAQ RMAQLRLVLG LHTLDSPGLT FHIKAAIQHP
110 120 130 140 150
RYKPVPALEN DLALLQLDGK VKPSRTIRPL ALPSKRQVVA AGTRCSMAGW
160 170 180 190 200
GLTHQGGRLS RVLRELDLQV LDTRMCNNSR FWNGSLSPSM VCLAADSKDQ
210 220 230 240 250
APCKGDSGGP LVCGKGRVLA RVLSFSSRVC TDIFKPPVAT AVAPYVSWIR

KVTGRSA
Length:257
Mass (Da):27,545
Last modified:May 18, 2010 - v2
Checksum:iB4E815CE455F7371
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051829221R → G.3 PublicationsCorresponds to variant rs1599882dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23134 mRNA. Translation: AAA59582.1.
L36922 Genomic DNA. Translation: AAA57262.1.
L36936 Genomic DNA. Translation: AAA57257.1.
AC011556 Genomic DNA. No translation available.
BC025701 mRNA. Translation: AAH25701.1.
CCDSiCCDS12031.1.
PIRiA55634.
RefSeqiNP_001245280.1. NM_001258351.1.
NP_005308.1. NM_005317.3.
UniGeneiHs.465511.

Genome annotation databases

EnsembliENST00000264553; ENSP00000264553; ENSG00000197540.
GeneIDi3004.
KEGGihsa:3004.
UCSCiuc002low.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23134 mRNA. Translation: AAA59582.1.
L36922 Genomic DNA. Translation: AAA57262.1.
L36936 Genomic DNA. Translation: AAA57257.1.
AC011556 Genomic DNA. No translation available.
BC025701 mRNA. Translation: AAH25701.1.
CCDSiCCDS12031.1.
PIRiA55634.
RefSeqiNP_001245280.1. NM_001258351.1.
NP_005308.1. NM_005317.3.
UniGeneiHs.465511.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LZPmodel-A26-257[»]
2ZGCX-ray1.96A26-257[»]
2ZGHX-ray2.17A26-257[»]
2ZGJX-ray2.30A26-257[»]
2ZKSX-ray2.70A26-257[»]
ProteinModelPortaliP51124.
SMRiP51124.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109259. 5 interactors.
IntActiP51124. 1 interactor.
STRINGi9606.ENSP00000264553.

Protein family/group databases

MEROPSiS01.139.

PTM databases

iPTMnetiP51124.
PhosphoSitePlusiP51124.

Polymorphism and mutation databases

BioMutaiGZMM.
DMDMi296434527.

Proteomic databases

PaxDbiP51124.
PeptideAtlasiP51124.
PRIDEiP51124.

Protocols and materials databases

DNASUi3004.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264553; ENSP00000264553; ENSG00000197540.
GeneIDi3004.
KEGGihsa:3004.
UCSCiuc002low.3. human.

Organism-specific databases

CTDi3004.
DisGeNETi3004.
GeneCardsiGZMM.
HGNCiHGNC:4712. GZMM.
HPAiHPA015624.
MIMi600311. gene.
neXtProtiNX_P51124.
OpenTargetsiENSG00000197540.
PharmGKBiPA29090.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP51124.
KOiK08649.
OMAiKDQAPCK.
OrthoDBiEOG091G0HUQ.
PhylomeDBiP51124.
TreeFamiTF335738.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000099805-MONOMER.
BRENDAi3.4.21.78. 2681.
3.4.21.B2. 2681.
ReactomeiR-HSA-173736. Alternative complement activation.

Miscellaneous databases

EvolutionaryTraceiP51124.
GeneWikiiGZMM.
GenomeRNAii3004.
PROiP51124.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000197540.
CleanExiHS_GZMM.
ExpressionAtlasiP51124. baseline and differential.
GenevisibleiP51124. HS.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR033040. GZMM.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PANTHERiPTHR24256:SF132. PTHR24256:SF132. 1 hit.
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRAM_HUMAN
AccessioniPrimary (citable) accession number: P51124
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 18, 2010
Last modified: November 30, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Peptidase families
    Classification of peptidase families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.