P51122 (AN32A_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acidic leucine-rich nuclear phosphoprotein 32 family member A

Alternative name(s):
Leucine-rich acidic nuclear protein
Short name=LANP
Potent heat-stable protein phosphatase 2A inhibitor I1PP2A

Gene names

Name:	ANP32A
Synonyms:	LANP, PHAP1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression By similarity.
Subunit structure	Component of the SET complex, which also contains SET, APEX1, HMGB2 and NME1. Directly interacts with SET. Interacts with ATXN1/SCA1. Interacts with MAP1B. Interacts with ELAVL1. Part of the INHAT (inhibitor of histone acetyltransferases) complex. Interacts with E4F1 By similarity.
Subcellular location	Nucleus. Cytoplasm. Endoplasmic reticulum By similarity. Note: Shuttles between nucleus and cytoplasm. Translocates to the cytoplasm during the process of neuritogenesis By similarity.
Tissue specificity	Widely distributed in the central nervous system, with an abundant expression in the cerebellum.
Post-translational modification	The N-terminus is blocked. Phosphorylated on serine residues By similarity. Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group By similarity.
Sequence similarities	Belongs to the ANP32 family. Contains 4 LRR (leucine-rich) repeats. Contains 1 LRRCT domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Endoplasmic reticulum Nucleus
Domain	Leucine-rich repeat Repeat
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	nucleocytoplasmic transport Inferred from sequence or structural similarity. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Acidic leucine-rich nuclear phosphoprotein 32 family member A		PRO_0000137591
Regions
	Repeat	18 – 38	21	LRR 1
	Repeat	43 – 64	22	LRR 2
	Repeat	65 – 87	23	LRR 3
	Repeat	89 – 110	22	LRR 4
	Domain	123 – 161	39	LRRCT
	Region	150 – 174	25	Necessary for tumor-suppressive function By similarity
	Region	165 – 249	85	Interaction with E4F1 By similarity
	Compositional bias	161 – 246	86	Glu-rich
Amino acid modifications
	Modified residue	15	1	Phosphothreonine By similarity
	Modified residue	17	1	Phosphoserine By similarity
	Modified residue	158	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51122 [UniParc]. Last modified June 13, 2006. Version 2. Checksum: 207914DC9AD2C38F	FASTA	249	28,617
	10 20 30 40 50 60 MDMDKRIHLE LRNRTPSDVK ELVLDNCRSN EGKIEGLTDE FEELEFLSTI NVGLTSVANL 70 80 90 100 110 120 PKLNKLKKLE LSDNRISGGL EVLAEKCPNL THLNLSGNKI KDLSTIEPLK KLENLKSLDL 130 140 150 160 170 180 FNCEVTNLND YRENVFKLLP QLTYLDGYDR DDKEAPDSDA EGYVEGLDDD EEDEDEEEYD 190 200 210 220 230 240 EDAQVVEDEE DEEEEEEGEE EDVSGEEEED EEGYNDGEVD DEEDEEELGE EERGQKRKRE PEDEGEDDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bovine embryonic ESTs." Lewin H.A., Renard J.P., Yang X.J., Hernandez A., Degrelle S., Hue I., Tian X.C., Liu L., Everts R.E. Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-201.
[2]	"Bovine genome sequencing program: full-length cDNA sequencing." Moore S., Alexander L., Brownstein M., Guan L., Lobo S., Meng Y., Tanaguchi M., Wang Z., Yu J., Prange C., Schreiber K., Shenmen C., Wagner L., Bala M., Barbazuk S., Barber S., Babakaiff R., Beland J. , Chun E., Del Rio L., Gibson S., Hanson R., Kirkpatrick R., Liu J., Matsuo C., Mayo M., Santos R.R., Stott J., Tsai M., Wong D., Siddiqui A., Holt R., Jones S.J., Marra M.A. Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 84-249.
[3]	"A nuclear factor containing the leucine-rich repeats expressed in murine cerebellar neurons." Matsuoka K., Taoka M., Satozawa N., Nakayama H., Ichimura T., Takahashi N., Yamakuni T., Song S.-Y., Isobe T. Proc. Natl. Acad. Sci. U.S.A. 91:9670-9674(1994) [PubMed: 7937870] [Abstract] Cited for: PROTEIN SEQUENCE OF 4-165 AND 235-245. Tissue: Brain.
[4]	"Molecular identification of I1PP2A, a novel potent heat-stable inhibitor protein of protein phosphatase 2A." Li M., Makkinje A., Damuni Z. Biochemistry 35:6998-7002(1996) [PubMed: 8679524] [Abstract] Cited for: PROTEIN SEQUENCE OF 7-12 AND 29-44. Tissue: Kidney.
[5]	"The Anp32 family of proteins containing leucine-rich repeats." Matilla A., Radrizzani M. Cerebellum 4:7-18(2005) [PubMed: 15895553] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CN437768 mRNA. No translation available. DV929633 mRNA. No translation available.
IPI	IPI00692911.
RefSeq	NP_001181948.1. NM_001195019.1.
UniGene	Bt.27190.
3D structure databases
ProteinModelPortal	P51122.
SMR	P51122. Positions 1-164.
ModBase	Search...
Protein-protein interaction databases
STRING	P51122.
Proteomic databases
PRIDE	P51122.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000016410; ENSBTAP00000016410; ENSBTAG00000012365.
GeneID	538427.
KEGG	bta:538427.
Organism-specific databases
CTD	8125.
Phylogenomic databases
eggNOG	maNOG14811.
GeneTree	ENSGT00560000077130.
HOVERGEN	HBG053102.
InParanoid	P51122.
OrthoDB	EOG4QNMXG.
Family and domain databases
InterPro	IPR001611. Leu-rich_rpt. IPR003603. U2A'_phosphoprotein32A_C. [Graphical view]
SMART	SM00446. LRRcap. 1 hit. [Graphical view]
PROSITE	PS51450. LRR. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AN32A_BOVIN

Accession

Primary (citable) accession number: P51122
Secondary accession number(s): P55930

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	June 13, 2006
Last modified:	November 16, 2011
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program