P51121 (GLNA_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine synthetase
Short name=GS
EC=6.3.1.2

Alternative name(s):
Glutamate decarboxylase
EC=4.1.1.15
Glutamate--ammonia ligase

Gene names

Name:

glul

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. May play an important role in the early stages of vertebrate neural development.
Catalytic activity	ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine. L-glutamate = 4-aminobutanoate + CO2.
Cofactor	Biotin By similarity. Magnesium or manganese By similarity.
Subunit structure	Homooctamer and homotetramer By similarity.
Subcellular location	Cytoplasm By similarity. Mitochondrion By similarity.
Developmental stage	Up-regulated during early neural development. Maternally transmitted and abundantly expressed in neuroectoderm-derived tissues during the gastrula and neurula stages.
Sequence similarities	Belongs to the glutamine synthetase family.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase Lyase
Gene Ontology (GO)
Biological_process	glutamine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate decarboxylase activity Inferred from electronic annotation. Source: EC glutamate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Glutamine synthetase		PRO_0000153146

Sequences

	Sequence		Length	Mass (Da)
	P51121 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: D69C175E95FA8D2F	FASTA	392	43,985
	10 20 30 40 50 60 MSVSHSSRLN KGVREQYMKL PQGEKVQVTY VWIDGTGEGV RCKTRTLDQE PKTIDEIPEW 70 80 90 100 110 120 NFDGSSTHQA EGSNSDMYLI PVQMFRDPFC LDPNKLVMCE VLKYNRKSAE TNLRHTCKKI 130 140 150 160 170 180 MEMVNDHRPW FGMEQEYTLL GINGHPYGWP ENGFPGPQGP YYCGVGADKV YGRDVVESHY 190 200 210 220 230 240 KACLYAGIKI CGTNAEVMPS QWEFQVGPCE GIDMGDHLWM ARFILHRVCE DFGVVATLDP 250 260 270 280 290 300 KPMTGNWNGA GCHTNYSTES MRVEGGLKHI EDAIEKLGKR HDYHICVYDP RGGKDNSRRL 310 320 330 340 350 360 TGQHETSSIH EFSAGVANRG ASIRIPRQVG QEGYGYFEDR RPAANCDPYA VTEALVRTTI 370 380 390 LNETGSETKD YKNGAGFSRA IGMASPRDAA VF

References

[1]	"Identification of a Xenopus glutamine synthetase gene abundantly expressed in the embryonic nervous system but not in adult brain." Hatada S., Kinoshita M., Noda M., Asashima M. FEBS Lett. 371:287-292(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50062 mRNA. Translation: BAA08779.1.
PIR	I51422.
RefSeq	NP_001082548.1. NM_001089079.1.
UniGene	Xl.47270.
3D structure databases
ProteinModelPortal	P51121.
SMR	P51121. Positions 4-372.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	398556.
KEGG	xla:398556.
Organism-specific databases
CTD	398556.
Xenbase	XB-GENE-6256042. glul.
Phylogenomic databases
HOVERGEN	HBG005847.
KO	K01915.
Family and domain databases
Gene3D	3.30.590.10. 1 hit.
InterPro	IPR008147. Gln_synt_beta. IPR014746. Gln_synth/guanido_kin_cat_dom. IPR008146. Gln_synth_cat_dom. IPR027303. Gln_synth_gly_rich_site. IPR027302. Gln_synth_N_conserv_site. [Graphical view]
Pfam	PF00120. Gln-synt_C. 1 hit. PF03951. Gln-synt_N. 1 hit. [Graphical view]
SUPFAM	SSF54368. Gln_synt_beta. 1 hit.
PROSITE	PS00180. GLNA_1. 1 hit. PS00181. GLNA_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNA_XENLA

Accession

Primary (citable) accession number: P51121

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program