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Protein

Glutamine synthetase

Gene

glul

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). May play an important role in the early stages of vertebrate neural development.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Protein has several cofactor binding sites:

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC
  3. glutamate decarboxylase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Lyase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase (EC:6.3.1.2)
Short name:
GS
Alternative name(s):
Glutamate decarboxylase (EC:4.1.1.15)
Glutamate--ammonia ligase
Gene namesi
Name:glul
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6256042. glul.

Subcellular locationi

  1. Cytoplasm By similarity
  2. Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Glutamine synthetasePRO_0000153146Add
BLAST

Expressioni

Developmental stagei

Up-regulated during early neural development. Maternally transmitted and abundantly expressed in neuroectoderm-derived tissues during the gastrula and neurula stages.

Interactioni

Subunit structurei

Homooctamer and homotetramer.By similarity

Structurei

3D structure databases

SMRiP51121. Positions 4-372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

HOVERGENiHBG005847.
KOiK01915.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51121-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSHSSRLN KGVREQYMKL PQGEKVQVTY VWIDGTGEGV RCKTRTLDQE
60 70 80 90 100
PKTIDEIPEW NFDGSSTHQA EGSNSDMYLI PVQMFRDPFC LDPNKLVMCE
110 120 130 140 150
VLKYNRKSAE TNLRHTCKKI MEMVNDHRPW FGMEQEYTLL GINGHPYGWP
160 170 180 190 200
ENGFPGPQGP YYCGVGADKV YGRDVVESHY KACLYAGIKI CGTNAEVMPS
210 220 230 240 250
QWEFQVGPCE GIDMGDHLWM ARFILHRVCE DFGVVATLDP KPMTGNWNGA
260 270 280 290 300
GCHTNYSTES MRVEGGLKHI EDAIEKLGKR HDYHICVYDP RGGKDNSRRL
310 320 330 340 350
TGQHETSSIH EFSAGVANRG ASIRIPRQVG QEGYGYFEDR RPAANCDPYA
360 370 380 390
VTEALVRTTI LNETGSETKD YKNGAGFSRA IGMASPRDAA VF
Length:392
Mass (Da):43,985
Last modified:October 1, 1996 - v1
Checksum:iD69C175E95FA8D2F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50062 mRNA. Translation: BAA08779.1.
PIRiI51422.
RefSeqiNP_001082548.1. NM_001089079.1.
UniGeneiXl.47270.

Genome annotation databases

GeneIDi398556.
KEGGixla:398556.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50062 mRNA. Translation: BAA08779.1.
PIRiI51422.
RefSeqiNP_001082548.1. NM_001089079.1.
UniGeneiXl.47270.

3D structure databases

SMRiP51121. Positions 4-372.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi398556.
KEGGixla:398556.

Organism-specific databases

CTDi398556.
XenbaseiXB-GENE-6256042. glul.

Phylogenomic databases

HOVERGENiHBG005847.
KOiK01915.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of a Xenopus glutamine synthetase gene abundantly expressed in the embryonic nervous system but not in adult brain."
    Hatada S., Kinoshita M., Noda M., Asashima M.
    FEBS Lett. 371:287-292(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiGLNA_XENLA
AccessioniPrimary (citable) accession number: P51121
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 1, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.