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Protein

Glutamine synthetase

Gene

glnA

Organism
Trichodesmium thiebautii
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

The activity of this enzyme could be controlled by adenylation under conditions of abundant glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei3ATPBy similarity1
Metal bindingi8Magnesium 2By similarity1
Metal bindingi16Magnesium 2By similarity1
Binding sitei61L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi65Magnesium 1; via pros nitrogenBy similarity1
Binding sitei69ATPBy similarity1
Binding sitei117L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi67 – 69ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I betaBy similarity
Short name:
GSI betaBy similarity
Gene namesi
Name:glnABy similarity
OrganismiTrichodesmium thiebautii
Taxonomic identifieri1208 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesMicrocoleaceaeTrichodesmium

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000153218‹1 – ›119Glutamine synthetaseAdd BLAST›119

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

Structurei

3D structure databases

ProteinModelPortaliP51120.
SMRiP51120.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni60 – 61L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.By similarity

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
PROSITEiView protein in PROSITE
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Fragment.

P51120-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
PIEKHHHEVA TAGQNEIACQ FNTLVKKADE IQVYKYVVHN VAHAYGQTAT
60 70 80 90 100
FMPKPLVGDN GSGMHCHQSI SKDGVNIFAG DKYAGLSEEA LYYIGGIIKH
110
ARAINAFRNA STNSYKRLS
Length:119
Mass (Da):13,075
Last modified:October 1, 1996 - v1
Checksum:iAED9FDA685C8770A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Non-terminal residuei1191

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30820 Genomic DNA. Translation: AAA77684.1.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLN1B_TRITH
AccessioniPrimary (citable) accession number: P51120
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 12, 2017
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families