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Protein

Dihydroflavonol 4-reductase

Gene

DFR

Organism
Vitis vinifera (Grape)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional enzyme involved in flavonoid metabolism.By similarity

Catalytic activityi

A (2R,3S,4S)-leucoanthocyanidin + NADP+ = a (2R,3R)-dihydroflavonol + NADPH.By similarity
(2S)-flavan-4-ol + NADP+ = (2S)-flavanone + NADPH.By similarity

Pathwayi: anthocyanin biosynthesis

This protein is involved in the pathway anthocyanin biosynthesis, which is part of Pigment biosynthesis.
View all proteins of this organism that are known to be involved in the pathway anthocyanin biosynthesis and in Pigment biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37NADPCombined sources1 Publication1
Binding sitei44NADPCombined sources1 Publication1
Binding sitei128SubstrateCombined sources1 Publication1
Binding sitei133SubstrateCombined sources1 Publication1
Binding sitei163SubstrateCombined sources1 Publication1
Active sitei167Proton donorBy similarity1
Binding sitei167NADPCombined sources1 Publication1
Binding sitei204Substrate; via carbonyl oxygenCombined sources1 Publication1
Binding sitei205NADPCombined sources1 Publication1
Binding sitei208SubstrateCombined sources1 Publication1
Binding sitei227SubstrateCombined sources1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 18NADPCombined sources1 Publication7
Nucleotide bindingi64 – 65NADPCombined sources1 Publication2
Nucleotide bindingi84 – 86NADPCombined sources1 Publication3
Nucleotide bindingi190 – 193NADPCombined sources1 Publication4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Flavonoid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00009.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroflavonol 4-reductase (EC:1.1.1.219By similarity)
Short name:
DFR
Alternative name(s):
Dihydrokaempferol 4-reductase
Flavanone 4-reductase (EC:1.1.1.234By similarity)
Short name:
FNR
Gene namesi
Name:DFR
OrganismiVitis vinifera (Grape)
Taxonomic identifieri29760 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsVitalesVitaceaeVitis

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002155721 – 337Dihydroflavonol 4-reductaseAdd BLAST337

Proteomic databases

PRIDEiP51110.

Expressioni

Inductioni

By light.

Interactioni

Protein-protein interaction databases

STRINGi29760.VIT_18s0001g12800.t01.

Structurei

Secondary structure

1337
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 12Combined sources6
Helixi16 – 27Combined sources12
Beta strandi31 – 37Combined sources7
Helixi42 – 49Combined sources8
Helixi54 – 57Combined sources4
Beta strandi58 – 62Combined sources5
Turni68 – 71Combined sources4
Helixi72 – 75Combined sources4
Beta strandi79 – 83Combined sources5
Helixi95 – 98Combined sources4
Helixi100 – 116Combined sources17
Beta strandi121 – 126Combined sources6
Helixi129 – 131Combined sources3
Beta strandi135 – 137Combined sources3
Beta strandi140 – 142Combined sources3
Helixi150 – 156Combined sources7
Helixi161 – 180Combined sources20
Beta strandi185 – 190Combined sources6
Beta strandi192 – 195Combined sources4
Beta strandi198 – 201Combined sources4
Helixi204 – 209Combined sources6
Helixi211 – 214Combined sources4
Helixi217 – 219Combined sources3
Turni221 – 224Combined sources4
Beta strandi225 – 230Combined sources6
Helixi231 – 243Combined sources13
Beta strandi249 – 253Combined sources5
Beta strandi256 – 259Combined sources4
Helixi260 – 270Combined sources11
Helixi295 – 299Combined sources5
Helixi308 – 321Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IODX-ray2.06A/B/C/D1-337[»]
2NNLX-ray2.10D/F1-337[»]
3BXXX-ray2.90A/B/C/D/E/F1-337[»]
3C1TX-ray2.25A/B/C/D1-337[»]
ProteinModelPortaliP51110.
SMRiP51110.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP51110.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1502. Eukaryota.
COG0451. LUCA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR001509. Epimerase_deHydtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01370. Epimerase. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

P51110-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSQSETVCV TGASGFIGSW LVMRLLERRL TVRATVRDPT NVKKVKHLLD
60 70 80 90 100
LPKAETHLTL WKADLADEGS FDEAIKGCTG VFHVATPMDF ESKDPENEVI
110 120 130 140 150
KPTIEGMLGI MKSCAAAKTV RRLVFTSSAG TVNIQEHQLP VYDESCWSDM
160 170 180 190 200
EFCRAKKMTA WMYFVSKTLA EQAAWKYAKE NNIDFITIIP TLVVGPFIMS
210 220 230 240 250
SMPPSLITAL SPITGNEAHY SIIRQGQFVH LDDLCNAHIY LFENPKAEGR
260 270 280 290 300
YICSSHDCII LDLAKMLREK YPEYNIPTEF KGVDENLKSV CFSSKKLTDL
310 320 330
GFEFKYSLED MFTGAVDTCR AKGLLRPSHE KPVDGKT
Length:337
Mass (Da):37,757
Last modified:October 1, 1996 - v1
Checksum:i73EDB67A03DF94E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75964 mRNA. Translation: CAA53578.1.
UniGeneiVvi.120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75964 mRNA. Translation: CAA53578.1.
UniGeneiVvi.120.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IODX-ray2.06A/B/C/D1-337[»]
2NNLX-ray2.10D/F1-337[»]
3BXXX-ray2.90A/B/C/D/E/F1-337[»]
3C1TX-ray2.25A/B/C/D1-337[»]
ProteinModelPortaliP51110.
SMRiP51110.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi29760.VIT_18s0001g12800.t01.

Proteomic databases

PRIDEiP51110.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG1502. Eukaryota.
COG0451. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00009.

Miscellaneous databases

EvolutionaryTraceiP51110.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR001509. Epimerase_deHydtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01370. Epimerase. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDFRA_VITVI
AccessioniPrimary (citable) accession number: P51110
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.