ID   DFRA_CALCH              Reviewed;         364 AA.
AC   P51103;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   22-FEB-2023, entry version 79.
DE   RecName: Full=Dihydroflavonol 4-reductase;
DE            Short=DFR;
DE            EC=1.1.1.219 {ECO:0000250|UniProtKB:Q9XES5};
DE   AltName: Full=Dihydrokaempferol 4-reductase;
DE   AltName: Full=Flavanone 4-reductase;
DE            Short=FNR;
DE            EC=1.1.1.234 {ECO:0000250|UniProtKB:Q9XES5};
GN   Name=F;
OS   Callistephus chinensis (China aster) (Callistemma chinense).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Asteroideae; Astereae;
OC   Australasian lineages; Asterinae; Callistephus.
OX   NCBI_TaxID=13379;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=L 01; TISSUE=Petal;
RA   Min B., Sommer H., Forkmann G.;
RL   Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Bifunctional enzyme involved in flavonoid metabolism.
CC       {ECO:0000250|UniProtKB:Q9XES5}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (2R,3S,4S)-leucoanthocyanidin + NADP(+) = a (2R,3R)-
CC         dihydroflavonol + H(+) + NADPH; Xref=Rhea:RHEA:54444,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC         ChEBI:CHEBI:138176, ChEBI:CHEBI:138188; EC=1.1.1.219;
CC         Evidence={ECO:0000250|UniProtKB:Q9XES5};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2S)-flavan-4-ol + NADP(+) = (2S)-flavanone + H(+) + NADPH;
CC         Xref=Rhea:RHEA:11228, ChEBI:CHEBI:15378, ChEBI:CHEBI:15605,
CC         ChEBI:CHEBI:15606, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC         EC=1.1.1.234; Evidence={ECO:0000250|UniProtKB:Q9XES5};
CC   -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis.
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. Dihydroflavonol-4-reductase subfamily. {ECO:0000305}.
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DR   EMBL; Z67981; CAA91922.1; -; mRNA.
DR   AlphaFoldDB; P51103; -.
DR   SMR; P51103; -.
DR   BRENDA; 1.1.1.219; 1066.
DR   UniPathway; UPA00009; -.
DR   GO; GO:0045552; F:dihydrokaempferol 4-reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0047890; F:flavanone 4-reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009718; P:anthocyanin-containing compound biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd08958; FR_SDR_e; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR001509; Epimerase_deHydtase.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   PANTHER; PTHR10366:SF613; DIHYDROFLAVONOL 4-REDUCTASE; 1.
DR   PANTHER; PTHR10366; NAD DEPENDENT EPIMERASE/DEHYDRATASE; 1.
DR   Pfam; PF01370; Epimerase; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE   2: Evidence at transcript level;
KW   Flavonoid biosynthesis; NADP; Oxidoreductase.
FT   CHAIN           1..364
FT                   /note="Dihydroflavonol 4-reductase"
FT                   /id="PRO_0000215564"
FT   BINDING         45
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:A0A059TC02"
FT   BINDING         164
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:A0A059TC02"
SQ   SEQUENCE   364 AA;  40726 MW;  83E82B02F1AF4329 CRC64;
     MKEDSPPTVC VTGAAGFIGS WLVMRLLERG YIVRATVRNP GDMKKVKHLL ELPKAETNLT
     LWKADLTQEG SFDEAIEGCH GVFHVATPMD FESKDPENEI IKPTIEGILS IIRSCAKAKT
     VKKLVYTSSA GTVNVQETQL PVYDESHWSD LDFIYSKKMT AWMYFVSKTL AEKAAMEAAK
     ENNIDFVSII PPLVVGPFIN PTFPPSLITA LSLINGAESH YSIIKQGQYV HLDDLCECHI
     FLYENPEAKG RYICSKQDAT IHQLARMIKQ KWPEYHVPTQ FAGIDEELPT VSFSSKKLID
     MGFKFKYDLE DMFKGAIDSC KEKGFLPYST NEVKKGLFES SINGNVHGQK GNQKIGDEGV
     KLVN
//