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P51102

- DFRA_ARATH

UniProt

P51102 - DFRA_ARATH

Protein

Dihydroflavonol-4-reductase

Gene

DFRA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (11 Jul 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Cis-3,4-leucopelargonidin + NADP+ = (+)-dihydrokaempferol + NADPH.

    Pathwayi

    GO - Molecular functioni

    1. coenzyme binding Source: InterPro
    2. dihydrokaempferol 4-reductase activity Source: TAIR
    3. protein binding Source: IntAct

    GO - Biological processi

    1. anthocyanin-containing compound biosynthetic process Source: TAIR
    2. cellular metabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Flavonoid biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00009.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydroflavonol-4-reductase (EC:1.1.1.219)
    Short name:
    DFR
    Alternative name(s):
    Dihydrokaempferol 4-reductase
    Protein TRANSPARENT TESTA 3
    Gene namesi
    Name:DFRA
    Synonyms:DFR, TT3
    Ordered Locus Names:At5g42800
    ORF Names:MJB21.18
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G42800.

    Subcellular locationi

    GO - Cellular componenti

    1. extrinsic component of endoplasmic reticulum membrane Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 382382Dihydroflavonol-4-reductasePRO_0000215563Add
    BLAST

    Proteomic databases

    PaxDbiP51102.
    PRIDEiP51102.

    Expressioni

    Gene expression databases

    GenevestigatoriP51102.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CHSP131144EBI-1546795,EBI-1546775

    Protein-protein interaction databases

    IntActiP51102. 4 interactions.
    MINTiMINT-8184604.

    Structurei

    3D structure databases

    ProteinModelPortaliP51102.
    SMRiP51102. Positions 6-328.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0451.
    InParanoidiP51102.
    KOiK13082.
    OMAiMYFVSKS.
    PhylomeDBiP51102.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR001509. Epimerase_deHydtase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01370. Epimerase. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P51102-1 [UniParc]FASTAAdd to Basket

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    MVSQKETVCV TGASGFIGSW LVMRLLERGY FVRATVRDPG NLKKVQHLLD    50
    LPNAKTLLTL WKADLSEEGS YDDAINGCDG VFHVATPMDF ESKDPENEVI 100
    KPTVNGMLGI MKACVKAKTV RRFVFTSSAG TVNVEEHQKN VYDENDWSDL 150
    EFIMSKKMTG WMYFVSKTLA EKAAWDFAEE KGLDFISIIP TLVVGPFITT 200
    SMPPSLITAL SPITRNEAHY SIIRQGQYVH LDDLCNAHIF LYEQAAAKGR 250
    YICSSHDATI LTISKFLRPK YPEYNVPSTF EGVDENLKSI EFSSKKLTDM 300
    GFNFKYSLEE MFIESIETCR QKGFLPVSLS YQSISEIKTK NENIDVKTGD 350
    GLTDGMKPCN KTETGITGER TDAPMLAQQM CA 382
    Length:382
    Mass (Da):42,775
    Last modified:July 11, 2002 - v2
    Checksum:i51F81F96512ECC3F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti57 – 571L → Q in AAA32783. (PubMed:1354004)Curated
    Sequence conflicti57 – 571L → Q in BAA85261. 1 PublicationCurated
    Sequence conflicti168 – 1681T → S in AAA32783. (PubMed:1354004)Curated
    Sequence conflicti168 – 1681T → S in BAA85261. 1 PublicationCurated
    Sequence conflicti299 – 2991D → E in AAA32783. (PubMed:1354004)Curated
    Sequence conflicti299 – 2991D → E in BAA85261. 1 PublicationCurated
    Sequence conflicti338 – 3381K → KVP in AAA32783. (PubMed:1354004)Curated
    Sequence conflicti338 – 3381K → KVP in BAA85261. 1 PublicationCurated
    Sequence conflicti343 – 3431N → I in AAA32783. (PubMed:1354004)Curated
    Sequence conflicti343 – 3431N → I in BAA85261. 1 PublicationCurated
    Sequence conflicti345 – 3451D → E in AAA32783. (PubMed:1354004)Curated
    Sequence conflicti345 – 3451D → E in BAA85261. 1 PublicationCurated
    Sequence conflicti366 – 3661I → V in AAA32783. (PubMed:1354004)Curated
    Sequence conflicti366 – 3661I → V in BAA85261. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86359 Genomic DNA. Translation: AAA32783.1.
    AB033294 Genomic DNA. Translation: BAA85261.1.
    AJ251982 Genomic DNA. Translation: CAC10525.1.
    AB007647 Genomic DNA. Translation: BAB10636.1.
    CP002688 Genomic DNA. Translation: AED94866.1.
    PIRiJQ1688.
    RefSeqiNP_199094.1. NM_123645.3.
    UniGeneiAt.23537.
    At.74948.

    Genome annotation databases

    EnsemblPlantsiAT5G42800.1; AT5G42800.1; AT5G42800.
    GeneIDi834291.
    KEGGiath:AT5G42800.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86359 Genomic DNA. Translation: AAA32783.1 .
    AB033294 Genomic DNA. Translation: BAA85261.1 .
    AJ251982 Genomic DNA. Translation: CAC10525.1 .
    AB007647 Genomic DNA. Translation: BAB10636.1 .
    CP002688 Genomic DNA. Translation: AED94866.1 .
    PIRi JQ1688.
    RefSeqi NP_199094.1. NM_123645.3.
    UniGenei At.23537.
    At.74948.

    3D structure databases

    ProteinModelPortali P51102.
    SMRi P51102. Positions 6-328.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P51102. 4 interactions.
    MINTi MINT-8184604.

    Proteomic databases

    PaxDbi P51102.
    PRIDEi P51102.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G42800.1 ; AT5G42800.1 ; AT5G42800 .
    GeneIDi 834291.
    KEGGi ath:AT5G42800.

    Organism-specific databases

    TAIRi AT5G42800.

    Phylogenomic databases

    eggNOGi COG0451.
    InParanoidi P51102.
    KOi K13082.
    OMAi MYFVSKS.
    PhylomeDBi P51102.

    Enzyme and pathway databases

    UniPathwayi UPA00009 .

    Gene expression databases

    Genevestigatori P51102.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR001509. Epimerase_deHydtase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF01370. Epimerase. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Effects of ionizing radiation on a plant genome: analysis of two Arabidopsis transparent testa mutations."
      Shirley B.W., Hanley S., Goodman H.M.
      Plant Cell 4:333-347(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia and cv. Landsberg erecta.
    2. "Structure of dihydroflavonol 4-reductase (DFR) gene and its flanking sequences from Arabidopsis thaliana ecotype Landsberg erecta."
      Ichikawa H., Okano E., Shirley B.W.
      Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Landsberg erecta.
    3. "Physiological and molecular characterization of transparent testa mutants of Arabidopsis impaired in phenylpropanoid pathway."
      Bharti A.K., Khurana J.P.
      Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Est.
    4. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
      Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    6. "The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic diversity."
      Saito K., Yonekura-Sakakibara K., Nakabayashi R., Higashi Y., Yamazaki M., Tohge T., Fernie A.R.
      Plant Physiol. Biochem. 0:0-0(2013)
      Cited for: REVIEW, NOMENCLATURE.

    Entry informationi

    Entry nameiDFRA_ARATH
    AccessioniPrimary (citable) accession number: P51102
    Secondary accession number(s): Q9FEB1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: July 11, 2002
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3