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Protein

Dihydroflavonol 4-reductase

Gene

DFRA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bifunctional enzyme involved in flavonoid metabolism.By similarity

Catalytic activityi

A (2R,3S,4S)-leucoanthocyanidin + NADP+ = a (2R,3R)-dihydroflavonol + NADPH.By similarity
(2S)-flavan-4-ol + NADP+ = (2S)-flavanone + NADPH.By similarity

Pathwayi: anthocyanin biosynthesis

This protein is involved in the pathway anthocyanin biosynthesis, which is part of Pigment biosynthesis.
View all proteins of this organism that are known to be involved in the pathway anthocyanin biosynthesis and in Pigment biosynthesis.

GO - Molecular functioni

  • coenzyme binding Source: InterPro
  • dihydrokaempferol 4-reductase activity Source: TAIR
  • flavanone 4-reductase activity Source: UniProtKB-EC

GO - Biological processi

  • anthocyanin-containing compound biosynthetic process Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processFlavonoid biosynthesis
LigandNADP

Enzyme and pathway databases

BRENDAi1.1.1.219 399
UniPathwayiUPA00009

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroflavonol 4-reductase (EC:1.1.1.219By similarity)
Short name:
DFR
Alternative name(s):
Dihydrokaempferol 4-reductase
Flavanone 4-reductase (EC:1.1.1.234By similarity)
Short name:
FNR
Protein TRANSPARENT TESTA 3
Gene namesi
Name:DFRA
Synonyms:DFR, TT3
Ordered Locus Names:At5g42800
ORF Names:MJB21.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G42800
TAIRilocus:2165427 AT5G42800

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002155631 – 382Dihydroflavonol 4-reductaseAdd BLAST382

Proteomic databases

PaxDbiP51102

Expressioni

Gene expression databases

ExpressionAtlasiP51102 baseline and differential
GenevisibleiP51102 AT

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CHSP131144EBI-1546795,EBI-1546775

Protein-protein interaction databases

BioGridi19541, 4 interactors
IntActiP51102, 4 interactors
MINTiP51102
STRINGi3702.AT5G42800.1

Structurei

3D structure databases

ProteinModelPortaliP51102
SMRiP51102
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1502 Eukaryota
COG0451 LUCA
InParanoidiP51102
KOiK13082
OMAiKERLTLW
OrthoDBiEOG09360E10
PhylomeDBiP51102

Family and domain databases

InterProiView protein in InterPro
IPR001509 Epimerase_deHydtase
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF01370 Epimerase, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit

Sequencei

Sequence statusi: Complete.

P51102-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSQKETVCV TGASGFIGSW LVMRLLERGY FVRATVRDPG NLKKVQHLLD
60 70 80 90 100
LPNAKTLLTL WKADLSEEGS YDDAINGCDG VFHVATPMDF ESKDPENEVI
110 120 130 140 150
KPTVNGMLGI MKACVKAKTV RRFVFTSSAG TVNVEEHQKN VYDENDWSDL
160 170 180 190 200
EFIMSKKMTG WMYFVSKTLA EKAAWDFAEE KGLDFISIIP TLVVGPFITT
210 220 230 240 250
SMPPSLITAL SPITRNEAHY SIIRQGQYVH LDDLCNAHIF LYEQAAAKGR
260 270 280 290 300
YICSSHDATI LTISKFLRPK YPEYNVPSTF EGVDENLKSI EFSSKKLTDM
310 320 330 340 350
GFNFKYSLEE MFIESIETCR QKGFLPVSLS YQSISEIKTK NENIDVKTGD
360 370 380
GLTDGMKPCN KTETGITGER TDAPMLAQQM CA
Length:382
Mass (Da):42,775
Last modified:July 11, 2002 - v2
Checksum:i51F81F96512ECC3F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57L → Q in AAA32783 (PubMed:1354004).Curated1
Sequence conflicti57L → Q in BAA85261 (Ref. 2) Curated1
Sequence conflicti168T → S in AAA32783 (PubMed:1354004).Curated1
Sequence conflicti168T → S in BAA85261 (Ref. 2) Curated1
Sequence conflicti299D → E in AAA32783 (PubMed:1354004).Curated1
Sequence conflicti299D → E in BAA85261 (Ref. 2) Curated1
Sequence conflicti338K → KVP in AAA32783 (PubMed:1354004).Curated1
Sequence conflicti338K → KVP in BAA85261 (Ref. 2) Curated1
Sequence conflicti343N → I in AAA32783 (PubMed:1354004).Curated1
Sequence conflicti343N → I in BAA85261 (Ref. 2) Curated1
Sequence conflicti345D → E in AAA32783 (PubMed:1354004).Curated1
Sequence conflicti345D → E in BAA85261 (Ref. 2) Curated1
Sequence conflicti366I → V in AAA32783 (PubMed:1354004).Curated1
Sequence conflicti366I → V in BAA85261 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86359 Genomic DNA Translation: AAA32783.1
AB033294 Genomic DNA Translation: BAA85261.1
AJ251982 Genomic DNA Translation: CAC10525.1
AB007647 Genomic DNA Translation: BAB10636.1
CP002688 Genomic DNA Translation: AED94866.1
PIRiJQ1688
RefSeqiNP_199094.1, NM_123645.4
UniGeneiAt.23537
At.74948

Genome annotation databases

EnsemblPlantsiAT5G42800.1; AT5G42800.1; AT5G42800
GeneIDi834291
GrameneiAT5G42800.1; AT5G42800.1; AT5G42800
KEGGiath:AT5G42800

Similar proteinsi

Entry informationi

Entry nameiDFRA_ARATH
AccessioniPrimary (citable) accession number: P51102
Secondary accession number(s): Q9FEB1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 11, 2002
Last modified: April 25, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health