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P51102 (DFRA_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydroflavonol-4-reductase

Short name=DFR
EC=1.1.1.219
Alternative name(s):
Dihydrokaempferol 4-reductase
Protein TRANSPARENT TESTA 3
Gene names
Name:DFRA
Synonyms:DFR, TT3
Ordered Locus Names:At5g42800
ORF Names:MJB21.18
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Cis-3,4-leucopelargonidin + NADP+ = (+)-dihydrokaempferol + NADPH.

Pathway

Pigment biosynthesis; anthocyanin biosynthesis.

Sequence similarities

Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CHSP131144EBI-1546795,EBI-1546775

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 382382Dihydroflavonol-4-reductase
PRO_0000215563

Experimental info

Sequence conflict571L → Q in AAA32783. Ref.1
Sequence conflict571L → Q in BAA85261. Ref.2
Sequence conflict1681T → S in AAA32783. Ref.1
Sequence conflict1681T → S in BAA85261. Ref.2
Sequence conflict2991D → E in AAA32783. Ref.1
Sequence conflict2991D → E in BAA85261. Ref.2
Sequence conflict3381K → KVP in AAA32783. Ref.1
Sequence conflict3381K → KVP in BAA85261. Ref.2
Sequence conflict3431N → I in AAA32783. Ref.1
Sequence conflict3431N → I in BAA85261. Ref.2
Sequence conflict3451D → E in AAA32783. Ref.1
Sequence conflict3451D → E in BAA85261. Ref.2
Sequence conflict3661I → V in AAA32783. Ref.1
Sequence conflict3661I → V in BAA85261. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P51102 [UniParc].

Last modified July 11, 2002. Version 2.
Checksum: 51F81F96512ECC3F

FASTA38242,775
        10         20         30         40         50         60 
MVSQKETVCV TGASGFIGSW LVMRLLERGY FVRATVRDPG NLKKVQHLLD LPNAKTLLTL 

        70         80         90        100        110        120 
WKADLSEEGS YDDAINGCDG VFHVATPMDF ESKDPENEVI KPTVNGMLGI MKACVKAKTV 

       130        140        150        160        170        180 
RRFVFTSSAG TVNVEEHQKN VYDENDWSDL EFIMSKKMTG WMYFVSKTLA EKAAWDFAEE 

       190        200        210        220        230        240 
KGLDFISIIP TLVVGPFITT SMPPSLITAL SPITRNEAHY SIIRQGQYVH LDDLCNAHIF 

       250        260        270        280        290        300 
LYEQAAAKGR YICSSHDATI LTISKFLRPK YPEYNVPSTF EGVDENLKSI EFSSKKLTDM 

       310        320        330        340        350        360 
GFNFKYSLEE MFIESIETCR QKGFLPVSLS YQSISEIKTK NENIDVKTGD GLTDGMKPCN 

       370        380 
KTETGITGER TDAPMLAQQM CA 

« Hide

References

« Hide 'large scale' references
[1]"Effects of ionizing radiation on a plant genome: analysis of two Arabidopsis transparent testa mutations."
Shirley B.W., Hanley S., Goodman H.M.
Plant Cell 4:333-347(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia and cv. Landsberg erecta.
[2]"Structure of dihydroflavonol 4-reductase (DFR) gene and its flanking sequences from Arabidopsis thaliana ecotype Landsberg erecta."
Ichikawa H., Okano E., Shirley B.W.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Landsberg erecta.
[3]"Physiological and molecular characterization of transparent testa mutants of Arabidopsis impaired in phenylpropanoid pathway."
Bharti A.K., Khurana J.P.
Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Est.
[4]"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[6]"The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic diversity."
Saito K., Yonekura-Sakakibara K., Nakabayashi R., Higashi Y., Yamazaki M., Tohge T., Fernie A.R.
Plant Physiol. Biochem. 0:0-0(2013)
Cited for: REVIEW, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M86359 Genomic DNA. Translation: AAA32783.1.
AB033294 Genomic DNA. Translation: BAA85261.1.
AJ251982 Genomic DNA. Translation: CAC10525.1.
AB007647 Genomic DNA. Translation: BAB10636.1.
CP002688 Genomic DNA. Translation: AED94866.1.
PIRJQ1688.
RefSeqNP_199094.1. NM_123645.3.
UniGeneAt.23537.
At.74948.

3D structure databases

ProteinModelPortalP51102.
SMRP51102. Positions 6-328.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP51102. 4 interactions.
MINTMINT-8184604.

Proteomic databases

PaxDbP51102.
PRIDEP51102.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G42800.1; AT5G42800.1; AT5G42800.
GeneID834291.
KEGGath:AT5G42800.

Organism-specific databases

TAIRAT5G42800.

Phylogenomic databases

eggNOGCOG0451.
InParanoidP51102.
KOK13082.
OMAMYFVSKS.
PhylomeDBP51102.
ProtClustDBPLN02650.

Enzyme and pathway databases

UniPathwayUPA00009.

Gene expression databases

GenevestigatorP51102.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR001509. Epimerase_deHydtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF01370. Epimerase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDFRA_ARATH
AccessionPrimary (citable) accession number: P51102
Secondary accession number(s): Q9FEB1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 11, 2002
Last modified: April 16, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names