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Protein

Anthocyanidin 3-O-glucosyltransferase 2

Gene

UFGT

Organism
Vitis vinifera (Grape)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In the presence of other necessary color factors, this glycosylation reaction allows the accumulation of anthocyanin pigments. Involved in the formation of red wine pigments. Can use UDP-Glc, UDP-5SGlc, UDP-Xyl, UDP-Man, UDP-Gal, UDP-GlcNAc, GDP-Glc, dTDP-Glc and dTDP-Xyl as sugar donor, but not UDP-6OMeGal, UDP-Ara, UDP-6FGal, UDP-GlcN, UDP-2FGal, UDP-5SAra, GDP-Man, GDPFuc, UDP-Fuc or UDP-Rha. Cyanidin is the natural acceptor, but quercitin and kaempferol can also be glucosylated.

Catalytic activityi

UDP-D-glucose + an anthocyanidin = UDP + an anthocyanidin-3-O-beta-D-glucoside.

Enzyme regulationi

Inhibited by Mn2+ and Zn2+.1 Publication

Kineticsi

  1. KM=679 µM for UDP-Glc (in the presence of 100 µM quercitin)2 Publications
  2. KM=300 µM for dTDP-Glc (in the presence of 100 µM quercitin)2 Publications
  3. KM=166 µM for UDP-5SGlc (in the presence of 100 µM quercitin)2 Publications
  4. KM=194 µM for UDP-GlcNAc (in the presence of 100 µM quercitin)2 Publications
  5. KM=48.2 µM for UDP-Gal (in the presence of 100 µM quercitin)2 Publications
  6. KM=50.1 µM for UDP-Man (in the presence of 100 µM quercitin)2 Publications
  7. KM=167 µM for GDP-Glc (in the presence of 100 µM quercitin)2 Publications
  8. KM=166 µM for dTDP-Xyl (in the presence of 100 µM quercitin)2 Publications
  9. KM=219 µM for UDP-Xyl (in the presence of 100 µM quercitin)2 Publications
  10. KM=30.8 µM for quercitin (in the presence of UDP-Glc)2 Publications
  11. KM=42.3 µM for kaempferol (in the presence of UDP-Glc)2 Publications
  12. KM=30 µM for cyanidin (in the presence of UDP-Glc)2 Publications
  13. KM=16 µM for delphinidin (in the presence of UDP-Glc)2 Publications
  14. KM=35.7 µM for malvidin (in the presence of UDP-Glc)2 Publications
  1. Vmax=18.9 nmol/sec/mg enzyme with quercitin as substrate2 Publications
  2. Vmax=905 nmol/sec/mg enzyme with cyanidin as substrate2 Publications

pH dependencei

Optimum pH is 8.0.2 Publications

Pathwayi: anthocyanin biosynthesis

This protein is involved in the pathway anthocyanin biosynthesis, which is part of Pigment biosynthesis.
View all proteins of this organism that are known to be involved in the pathway anthocyanin biosynthesis and in Pigment biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei18 – 181Substrate
Binding sitei19 – 191UDP-D-glucose1 Publication
Binding sitei20 – 201Substrate
Binding sitei84 – 841Substrate
Binding sitei141 – 1411UDP-D-glucose1 Publication
Binding sitei150 – 1501Substrate
Binding sitei188 – 1881Substrate
Binding sitei280 – 2801UDP-D-glucose1 Publication
Binding sitei306 – 3061UDP-D-glucose1 Publication
Binding sitei333 – 3331UDP-D-glucose; via amide nitrogen and carbonyl oxygen1 Publication

GO - Molecular functioni

  • anthocyanidin 3-O-glucosyltransferase activity Source: UniProtKB

GO - Biological processi

  • anthocyanin-containing compound biosynthetic process Source: UniProtKB
  • cyanidin 3-O-glucoside biosynthetic process Source: UniProtKB
  • kaempferol O-glucoside biosynthetic process Source: UniProtKB
  • quercetin O-glucoside biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.115. 6671.
UniPathwayiUPA00009.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthocyanidin 3-O-glucosyltransferase 2 (EC:2.4.1.115)
Alternative name(s):
Flavonol 3-O-glucosyltransferase
UDP-glucose flavonoid 3-O-glucosyltransferase
Gene namesi
Name:UFGT
Synonyms:AlUFGT1, AlUFGT2, FlUFGT1, FlUFGT2, ITUFGT1, ITUFGT2, RUUFGT1, RUUFGT2, VVGT1
ORF Names:GSVIVT00014047001, LOC100233099, VITISV_008354
OrganismiVitis vinifera (Grape)
Taxonomic identifieri29760 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsVitalesVitaceaeVitis

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi20 – 201H → A: Loss of catalytic activity. 1 Publication
Mutagenesisi141 – 1411T → A: 6-fold decrease in catalytic efficiency. 1 Publication
Mutagenesisi374 – 3741D → A: Loss of catalytic activity. 1 Publication
Mutagenesisi375 – 3751Q → H: Loss of catalytic activity. 1 Publication
Mutagenesisi375 – 3751Q → N: Impaired catalytic activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 456456Anthocyanidin 3-O-glucosyltransferase 2PRO_0000074151Add
BLAST

Expressioni

Tissue specificityi

Detected only in berry skin.1 Publication

Inductioni

By light.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi29760.VIT_16s0039g02230.t01.

Structurei

Secondary structure

1
456
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 135Combined sources
Beta strandi17 – 204Combined sources
Helixi21 – 3414Combined sources
Beta strandi38 – 447Combined sources
Helixi46 – 527Combined sources
Beta strandi64 – 685Combined sources
Helixi85 – 10925Combined sources
Beta strandi115 – 1195Combined sources
Helixi125 – 1328Combined sources
Beta strandi135 – 1406Combined sources
Helixi144 – 1518Combined sources
Helixi153 – 1608Combined sources
Helixi184 – 1863Combined sources
Beta strandi191 – 1944Combined sources
Helixi199 – 21012Combined sources
Helixi211 – 2133Combined sources
Beta strandi217 – 2215Combined sources
Helixi223 – 2253Combined sources
Helixi227 – 23610Combined sources
Beta strandi240 – 2423Combined sources
Helixi246 – 2494Combined sources
Helixi261 – 2666Combined sources
Beta strandi273 – 2775Combined sources
Helixi286 – 29914Combined sources
Beta strandi303 – 3064Combined sources
Helixi309 – 3146Combined sources
Helixi319 – 3235Combined sources
Turni324 – 3263Combined sources
Beta strandi327 – 3315Combined sources
Helixi335 – 3395Combined sources
Beta strandi344 – 3496Combined sources
Helixi353 – 36210Combined sources
Beta strandi366 – 3683Combined sources
Helixi375 – 38410Combined sources
Beta strandi389 – 3913Combined sources
Helixi393 – 3953Combined sources
Helixi399 – 41113Combined sources
Helixi413 – 43220Combined sources
Helixi438 – 45013Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2C1XX-ray1.90A1-456[»]
2C1ZX-ray1.90A1-456[»]
2C9ZX-ray2.10A1-456[»]
ProteinModelPortaliP51094.
SMRiP51094. Positions 6-455.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP51094.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni350 – 3589UDP-D-glucose binding
Regioni374 – 3752UDP-D-glucose binding

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi32 – 354Poly-Ala

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Phylogenomic databases

eggNOGiKOG1192. Eukaryota.
COG1819. LUCA.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51094-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQTTTNPHV AVLAFPFSTH AAPLLAVVRR LAAAAPHAVF SFFSTSQSNA
60 70 80 90 100
SIFHDSMHTM QCNIKSYDIS DGVPEGYVFA GRPQEDIELF TRAAPESFRQ
110 120 130 140 150
GMVMAVAETG RPVSCLVADA FIWFAADMAA EMGLAWLPFW TAGPNSLSTH
160 170 180 190 200
VYIDEIREKI GVSGIQGRED ELLNFIPGMS KVRFRDLQEG IVFGNLNSLF
210 220 230 240 250
SRMLHRMGQV LPKATAVFIN SFEELDDSLT NDLKSKLKTY LNIGPFNLIT
260 270 280 290 300
PPPVVPNTTG CLQWLKERKP TSVVYISFGT VTTPPPAEVV ALSEALEASR
310 320 330 340 350
VPFIWSLRDK ARVHLPEGFL EKTRGYGMVV PWAPQAEVLA HEAVGAFVTH
360 370 380 390 400
CGWNSLWESV AGGVPLICRP FFGDQRLNGR MVEDVLEIGV RIEGGVFTKS
410 420 430 440 450
GLMSCFDQIL SQEKGKKLRE NLRALRETAD RAVGPKGSST ENFITLVDLV

SKPKDV
Length:456
Mass (Da):50,150
Last modified:October 13, 2009 - v2
Checksum:i60BF6A70A3D17ABC
GO

Sequence cautioni

The sequence CAA53582.1 differs from that shown. Reason: Frameshift at positions 307, 309, 341 and 344. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti47 – 471Q → Z in strain: cv. Pinot noir.
Natural varianti69 – 691I → V in strain: cv. Italia, cv. Pinot noir and cv. Ruby Okuyama.
Natural varianti74 – 741P → A in strain: cv. Pinot noir / PN40024.
Natural varianti91 – 911T → M in strain: cv. Flame Muscat, cv. Italia, cv. Muscat of Alexandria, cv. Pinot noir, cv. Pinot noir / PN40024, cv. Red Globe and cv. Ruby Okuyama.
Natural varianti134 – 1341L → V in strain: cv. Flame Muscat, cv. Italia, cv. Muscat of Alexandria, cv. Pinot noir, cv. Pinot noir / PN40024, cv. Red Globe and cv. Ruby Okuyama. 1 Publication
Natural varianti153 – 1531I → T in strain: cv. Flame Muscat, cv. Italia, cv. Muscat of Alexandria, cv. Pinot noir / PN40024, cv. Red Globe and cv. Ruby Okuyama.
Natural varianti161 – 1611G → A in cv. Red Globe.
Natural varianti163 – 1631S → SG in strain: cv. Pinot noir / PN40024.
Natural varianti255 – 2551V → I in strain: cv. Italia, cv. Pinot noir / PN40024 and cv. Ruby Okuyama.
Natural varianti289 – 2891V → L in strain: cv. Flame Muscat, cv. Italia, cv. Muscat of Alexandria, cv. Pinot noir, cv. Pinot noir / PN40024, cv. Red Globe and cv. Ruby Okuyama.
Natural varianti293 – 2931S → A in strain: cv. Flame Muscat, cv. Italia, cv. Muscat of Alexandria, cv. Pinot noir, cv. Pinot noir / PN40024, cv. Red Globe and cv. Ruby Okuyama.
Natural varianti312 – 3121R → S in strain: cv. Flame Muscat, cv. Lambrusco Foglia Frastagliata and cv. Muscat of Alexandria.
Natural varianti326 – 3261Y → H in cv. Red Globe.
Natural varianti372 – 3721F → Y in strain: cv. Pinot noir / PN40024.
Natural varianti385 – 3851V → A in strain: cv. Flame Muscat, cv. Italia, cv. Muscat of Alexandria, cv. Pinot noir, cv. Pinot noir / PN40024, cv. Red Globe and cv. Ruby Okuyama.
Natural varianti399 – 4002KS → EN in strain: cv. Flame Muscat and cv. Muscat of Alexandria.
Natural varianti399 – 3991K → E in strain: cv. Flame Muscat, cv. Italia, cv. Muscat of Alexandria, cv. Pinot noir, cv. Pinot noir / PN40024 and cv. Ruby Okuyama.
Natural varianti423 – 4231R → G in strain: cv. Pinot noir / PN40024.
Natural varianti444 – 4441I → K in strain: cv. Flame Muscat, cv. Italia, cv. Lambrusco Foglia Frastagliata, cv. Muscat of Alexandria, cv. Pinot noir, cv. Pinot noir / PN40024, cv. Red Globe and cv. Ruby Okuyama.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000371 mRNA. Translation: AAB81682.1.
AF000372 mRNA. Translation: AAB81683.1.
AB047092 Genomic DNA. Translation: BAB41019.1.
AB047093 Genomic DNA. Translation: BAB41020.1.
AB047094 Genomic DNA. Translation: BAB41021.1.
AB047095 Genomic DNA. Translation: BAB41022.1.
AB047096 Genomic DNA. Translation: BAB41023.1.
AB047097 Genomic DNA. Translation: BAB41024.1.
AB047098 Genomic DNA. Translation: BAB41025.1.
AB047099 Genomic DNA. Translation: BAB41026.1.
DQ513314 Genomic DNA. Translation: ABF59818.1.
AM472935 Genomic DNA. Translation: CAN61846.1.
X75968 mRNA. Translation: CAA53582.1. Frameshift.
UniGeneiVvi.17.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000371 mRNA. Translation: AAB81682.1.
AF000372 mRNA. Translation: AAB81683.1.
AB047092 Genomic DNA. Translation: BAB41019.1.
AB047093 Genomic DNA. Translation: BAB41020.1.
AB047094 Genomic DNA. Translation: BAB41021.1.
AB047095 Genomic DNA. Translation: BAB41022.1.
AB047096 Genomic DNA. Translation: BAB41023.1.
AB047097 Genomic DNA. Translation: BAB41024.1.
AB047098 Genomic DNA. Translation: BAB41025.1.
AB047099 Genomic DNA. Translation: BAB41026.1.
DQ513314 Genomic DNA. Translation: ABF59818.1.
AM472935 Genomic DNA. Translation: CAN61846.1.
X75968 mRNA. Translation: CAA53582.1. Frameshift.
UniGeneiVvi.17.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2C1XX-ray1.90A1-456[»]
2C1ZX-ray1.90A1-456[»]
2C9ZX-ray2.10A1-456[»]
ProteinModelPortaliP51094.
SMRiP51094. Positions 6-455.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi29760.VIT_16s0039g02230.t01.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG1192. Eukaryota.
COG1819. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00009.
BRENDAi2.4.1.115. 6671.

Miscellaneous databases

EvolutionaryTraceiP51094.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of Vitis vinifera UDP-glucose:flavonoid 3-O-glucosyltransferase, a homologue of the enzyme encoded by the maize Bronze-1 locus that may primarily serve to glucosylate anthocyanidins in vivo."
    Ford C.M., Boss P.K., Hoj P.B.
    J. Biol. Chem. 273:9224-9233(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, VARIANT VAL-134.
    Strain: cv. Shiraz.
    Tissue: Fruit.
  2. "Comparison of UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT) gene sequences between white grapes (Vitis vinifera) and their sports with red skin."
    Kobayashi S., Ishimaru M., Ding C.K., Yakushiji H., Goto N.
    Plant Sci. 160:543-550(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Flame Muscat, cv. Italia, cv. Muscat of Alexandria and cv. Ruby Okuyama.
  3. "Molecular cloning of UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT) gene from Vitis vinifera Red Globe."
    Cheng J.H., Wu J., Yang F.C.
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Red Globe.
  4. "The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla."
    Jaillon O., Aury J.-M., Noel B., Policriti A., Clepet C., Casagrande A., Choisne N., Aubourg S., Vitulo N., Jubin C., Vezzi A., Legeai F., Hugueney P., Dasilva C., Horner D., Mica E., Jublot D., Poulain J.
    , Bruyere C., Billault A., Segurens B., Gouyvenoux M., Ugarte E., Cattonaro F., Anthouard V., Vico V., Del Fabbro C., Alaux M., Di Gaspero G., Dumas V., Felice N., Paillard S., Juman I., Moroldo M., Scalabrin S., Canaguier A., Le Clainche I., Malacrida G., Durand E., Pesole G., Laucou V., Chatelet P., Merdinoglu D., Delledonne M., Pezzotti M., Lecharny A., Scarpelli C., Artiguenave F., Pe M.E., Valle G., Morgante M., Caboche M., Adam-Blondon A.-F., Weissenbach J., Quetier F., Wincker P.
    Nature 449:463-467(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Pinot noir / PN40024.
  5. "A high quality draft consensus sequence of the genome of a heterozygous grapevine variety."
    Velasco R., Zharkikh A., Troggio M., Cartwright D.A., Cestaro A., Pruss D., Pindo M., FitzGerald L.M., Vezzulli S., Reid J., Malacarne G., Iliev D., Coppola G., Wardell B., Micheletti D., Macalma T., Facci M., Mitchell J.T.
    , Perazzolli M., Eldredge G., Gatto P., Oyzerski R., Moretto M., Gutin N., Stefanini M., Chen Y., Segala C., Davenport C., Dematte L., Mraz A., Battilana J., Stormo K., Costa F., Tao Q., Si-Ammour A., Harkins T., Lackey A., Perbost C., Taillon B., Stella A., Solovyev V., Fawcett J.A., Sterck L., Vandepoele K., Grando S.M., Toppo S., Moser C., Lanchbury J., Bogden R., Skolnick M., Sgaramella V., Bhatnagar S.K., Fontana P., Gutin A., Van de Peer Y., Salamini F., Viola R.
    PLoS ONE 2:E1326-E1326(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Pinot noir.
  6. "Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.)."
    Sparvoli F., Martin C., Scienza A., Gavazzi G., Tonelli C.
    Plant Mol. Biol. 24:743-755(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 303-456, INDUCTION.
    Strain: cv. Lambrusco Foglia Frastagliata.
  7. "Expression of anthocyanin biosynthesis pathway genes in red and white grapes."
    Boss P.K., Davies C., Robinson S.P.
    Plant Mol. Biol. 32:565-569(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  8. "Structure of a flavonoid glucosyltransferase reveals the basis for plant natural product modification."
    Offen W., Martinez-Fleites C., Yang M., Kiat-Lim E., Davis B.G., Tarling C.A., Ford C.M., Bowles D.J., Davies G.J.
    EMBO J. 25:1396-1405(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH UDP-GLUCOSE-DERIVED DONOR AND KAEMPFEROL OR IN COMPLEX WITH UDP AND QUERCETIN, MUTAGENESIS OF HIS-20; THR-141; ASP-374 AND GLN-375, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiUFOG_VITVI
AccessioniPrimary (citable) accession number: P51094
Secondary accession number(s): A5BVQ6
, A7PBD4, O22303, O22304, Q1G141, Q9AQV0, Q9AR43, Q9AR45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 13, 2009
Last modified: November 11, 2015
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The expression of UFGT is not detected in white grapes.

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.