ID   LDOX_VITVI              Reviewed;         362 AA.
AC   P51093;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   05-MAY-2009, entry version 44.
DE   RecName: Full=Leucoanthocyanidin dioxygenase;
DE            Short=LDOX;
DE            Short=Leucocyanidin oxygenase;
DE            EC=1.14.11.19;
DE   AltName: Full=Leucoanthocyanidin hydroxylase;
OS   Vitis vinifera (Grape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   Vitales; Vitaceae; Vitis.
OX   NCBI_TaxID=29760;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Lambrusco Foglia Frastagliata;
RX   MEDLINE=94250837; PubMed=8193299; DOI=10.1007/BF00029856;
RA   Sparvoli F., Martin C., Scienza A., Gavazzi G., Tonelli C.;
RT   "Cloning and molecular analysis of structural genes involved in
RT   flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.).";
RL   Plant Mol. Biol. 24:743-755(1994).
CC   -!- FUNCTION: Oxidation of leucoanthocyanidins into anthocyanidins.
CC   -!- CATALYTIC ACTIVITY: Leucocyanidin + 2-oxoglutarate + O(2) = cis-
CC       and trans-dihydroquercetins + succinate + CO(2) + H(2)O.
CC   -!- COFACTOR: Binds 1 iron ion per subunit (By similarity).
CC   -!- COFACTOR: Binds 1 ascorbate molecule per subunit (By similarity).
CC   -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis.
CC   -!- INDUCTION: By light.
CC   -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC       family.
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DR   EMBL; X75966; CAA53580.1; -; mRNA.
DR   HSSP; Q96323; 1GP6.
DR   SMR; P51093; 8-321.
DR   BRENDA; 1.14.11.19; 81671.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
DR   GO; GO:0050589; F:leucocyanidin oxygenase activity; IEA:EC.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR005123; Oxoglutarate/Fe-dep_Oase.
DR   Pfam; PF03171; 2OG-FeII_Oxy; 1.
PE   2: Evidence at transcript level;
KW   Dioxygenase; Flavonoid biosynthesis; Iron; Metal-binding;
KW   Oxidoreductase; Vitamin C.
FT   CHAIN         1    362       Leucoanthocyanidin dioxygenase.
FT                                /FTId=PRO_0000067304.
FT   METAL       238    238       Iron (By similarity).
FT   METAL       240    240       Iron (By similarity).
FT   METAL       294    294       Iron (By similarity).
SQ   SEQUENCE   362 AA;  41036 MW;  D3D0686C386A9315 CRC64;
     MVTSVAPRVE SLSSSGIQSI PKEYIRPQEE LTSIGNVFEE EKKDEGPQVP TIDLKDIESE
     DEVVRREIRE RCREELKKAA MEWGVMHLVN HGISDDLINR VKVAGETFFN LPMEEKEKYA
     NDQASGKIAG YGSKLANNAS GQLEWEDYFF HLIFPEDKRD MTIWPKTPSD YVPATCEYSV
     KLRSLATKIL SVLSLGLGLE EGRLEKEVGG MEELLLQKKI NYYPKCPQPE LALGVEAHTD
     VSALTFILHN MVPGLQLFYE GKWVTAKCVP NSIIMHIGDT IEILSNGKYK SILHRGLVNK
     EKVRISWAVF CEPPKEKIIL KAHCQRRCLR LSHHSSHLAP FPNIFSTSSS GRPRRLYSPN
     EL
//