Reviewed, UniProtKB/Swiss-Prot P51093 (LDOX_VITVI)

Names and origin

Protein names	Recommended name:     Leucoanthocyanidin dioxygenase       Short name=LDOX       Short name=Leucocyanidin oxygenase     EC=1.14.11.19 Alternative name(s):     Leucoanthocyanidin hydroxylase
Organism	Vitis vinifera (Grape)
Taxonomic identifier	29760 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Vitales › Vitaceae › Vitis

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Oxidation of leucoanthocyanidins into anthocyanidins.
Catalytic activity	Leucocyanidin + 2-oxoglutarate + O(2) = cis- and trans-dihydroquercetins + succinate + CO(2) + H(2)O.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 ascorbate molecule per subunit By similarity.
Pathway	Pigment biosynthesis; anthocyanin biosynthesis.
Induction	By light.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW leucocyanidin oxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Leucoanthocyanidin dioxygenase		PRO_0000067304
Sites
	Metal binding	238	1	Iron By similarity
	Metal binding	240	1	Iron By similarity
	Metal binding	294	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51093-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: D3D0686C386A9315	FASTA	362	41,036
	10 20 30 40 50 60 MVTSVAPRVE SLSSSGIQSI PKEYIRPQEE LTSIGNVFEE EKKDEGPQVP TIDLKDIESE 70 80 90 100 110 120 DEVVRREIRE RCREELKKAA MEWGVMHLVN HGISDDLINR VKVAGETFFN LPMEEKEKYA 130 140 150 160 170 180 NDQASGKIAG YGSKLANNAS GQLEWEDYFF HLIFPEDKRD MTIWPKTPSD YVPATCEYSV 190 200 210 220 230 240 KLRSLATKIL SVLSLGLGLE EGRLEKEVGG MEELLLQKKI NYYPKCPQPE LALGVEAHTD 250 260 270 280 290 300 VSALTFILHN MVPGLQLFYE GKWVTAKCVP NSIIMHIGDT IEILSNGKYK SILHRGLVNK 310 320 330 340 350 360 EKVRISWAVF CEPPKEKIIL KAHCQRRCLR LSHHSSHLAP FPNIFSTSSS GRPRRLYSPN EL

References

[1]

"Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.)." Sparvoli F., Martin C., Scienza A., Gavazzi G., Tonelli C. Plant Mol. Biol. 24:743-755(1994) [PubMed: 8193299] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Lambrusco Foglia Frastagliata.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X75966 mRNA. Translation: CAA53580.1.
3D structure databases
HSSP	HSSP built from PDB template 1GP6 based on UniProtKB Q96323.
SMR	P51093. Positions 8-321.
ModBase	Search...
Family and domain databases
InterPro	IPR005123. 2OG-FeII_Oase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDOX_VITVI

Accession

Primary (citable) accession number: P51093

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 4, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)