Reviewed, UniProtKB/Swiss-Prot P51092 (LDOX_PETHY)

Names and origin

Protein names

Recommended name:
    Leucoanthocyanidin dioxygenase
      Short name=LDOX
      Short name=Leucocyanidin oxygenase
    EC=1.14.11.19
Alternative name(s):
    Leucoanthocyanidin hydroxylase

Gene names

Name:

ANT17

Organism

Petunia hybrida (Petunia)

Taxonomic identifier

4102 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Petunioideae › Petunia

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Oxidation of leucoanthocyanidins into anthocyanidins.
Catalytic activity	Leucocyanidin + 2-oxoglutarate + O(2) = cis- and trans-dihydroquercetins + succinate + CO(2) + H(2)O.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 ascorbate molecule per subunit By similarity.
Pathway	Pigment biosynthesis; anthocyanin biosynthesis.
Tissue specificity	Predominantly expressed in corollas and at lower levels in anthers.
Induction	By gibberellic acid (GA).
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW leucocyanidin oxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Leucoanthocyanidin dioxygenase		PRO_0000067303
Sites
	Metal binding	236	1	Iron By similarity
	Metal binding	238	1	Iron By similarity
	Metal binding	292	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51092-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 72AB3DA2ACA7D22C	FASTA	430	48,433
	10 20 30 40 50 60 MVNAVVTTPS RVESLAKSGI QAIPKEYVRP QEELNGIGNI FEEEKKDEGP QVPTIDLKEI 70 80 90 100 110 120 DSEDKEIREK CHQLKKAAME WGVMHLVNHG ISDELINRVK VAGETFFDQP VEEKEKYAND 130 140 150 160 170 180 QANGNVQGYG SKLANSACGQ LEWEDYFFHC AFPEDKRDLS IWPKNPTDYT PATSEYAKQI 190 200 210 220 230 240 RALATKILTV LSIGLGLEEG RLEKEVGGME DLLLQMKINY YPKCPQPELA LGVEAHTDVS 250 260 270 280 290 300 ALTFILHNMV PGLQLFYEGQ WVTAKCVPNS IIMHIGDTIE ILSNGKYKSI LHRGVVNKEK 310 320 330 340 350 360 VRFSWAIFCE PPKEKIILKP LPETVTEAEP PRFPPRTFAQ HMAHKLFRKD DKDAAVEHKV 370 380 390 400 410 420 FNEDELDTAA EHKVLKKDNQ DAVAENKDIK EDEQCGPAEH KDIKEDGQGA AAENKVFKEN 430 NQDVAAEESK

References

[1]

"The petunia homologue of the Antirrhinum majus candi and Zea mays A2 flavonoid genes; homology to flavanone 3-hydroxylase and ethylene-forming enzyme." Weiss D., van der Luit A.H., Kroon J.T.M., Mol J.N.M., Kooter J.M. Plant Mol. Biol. 22:893-897(1993) [PubMed: 8358035] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Violet 26. Tissue: Corolla.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X70786 mRNA. No translation available.
PIR	S36233.
3D structure databases
HSSP	HSSP built from PDB template 1GP5 based on UniProtKB Q96323.
SMR	P51092. Positions 11-353.
ModBase	Search...
Family and domain databases
InterPro	IPR005123. 2OG-FeII_Oase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDOX_PETHY

Accession

Primary (citable) accession number: P51092

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 4, 2008
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)