ID   LDOX_MALDO              Reviewed;         357 AA.
AC   P51091;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   05-MAY-2009, entry version 46.
DE   RecName: Full=Leucoanthocyanidin dioxygenase;
DE            Short=LDOX;
DE            Short=Leucocyanidin oxygenase;
DE            EC=1.14.11.19;
DE   AltName: Full=Leucoanthocyanidin hydroxylase;
DE   AltName: Full=Anthocyanidin synthase;
GN   Name=ANS;
OS   Malus domestica (Apple) (Malus sylvestris).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Rosales; Rosaceae; Maloideae; Malus.
OX   NCBI_TaxID=3750;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf;
RX   MEDLINE=94294547; PubMed=8022923; DOI=10.1104/pp.103.3.1015;
RA   Davies K.M.;
RT   "A Malus cDNA with homology to the Antirrhinum Candica and Zea A2
RT   genes.";
RL   Plant Physiol. 103:1015-1015(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Fuji; TISSUE=Peelings;
RA   Lee J.-R., Hong S.-T., Yoo Y.G., Kim S.-R.;
RT   "Molecular cloning and expression of anthocyanin biosynthesis genes
RT   from 'Fuji apple'.";
RL   Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Oxidation of leucoanthocyanidins into anthocyanidins.
CC   -!- CATALYTIC ACTIVITY: Leucocyanidin + 2-oxoglutarate + O(2) = cis-
CC       and trans-dihydroquercetins + succinate + CO(2) + H(2)O.
CC   -!- COFACTOR: Binds 1 iron ion per subunit (By similarity).
CC   -!- COFACTOR: Binds 1 ascorbate molecule per subunit (By similarity).
CC   -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis.
CC   -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC       family.
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DR   EMBL; X71360; CAA50498.1; -; mRNA.
DR   EMBL; AF117269; AAD26205.1; -; mRNA.
DR   PIR; S33144; S33144.
DR   HSSP; Q96323; 1GP6.
DR   SMR; P51091; 10-354.
DR   BRENDA; 1.14.11.19; 66979.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
DR   GO; GO:0050589; F:leucocyanidin oxygenase activity; IEA:EC.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR005123; Oxoglutarate/Fe-dep_Oase.
DR   Pfam; PF03171; 2OG-FeII_Oxy; 1.
PE   2: Evidence at transcript level;
KW   Dioxygenase; Flavonoid biosynthesis; Iron; Metal-binding;
KW   Oxidoreductase; Vitamin C.
FT   CHAIN         1    357       Leucoanthocyanidin dioxygenase.
FT                                /FTId=PRO_0000067301.
FT   METAL       236    236       Iron (By similarity).
FT   METAL       238    238       Iron (By similarity).
FT   METAL       292    292       Iron (By similarity).
SQ   SEQUENCE   357 AA;  40332 MW;  FA5D97C25267B3E6 CRC64;
     MVSSDSVNSR VETLAGSGIS TIPKEYIRPK DELVNIGDIF EQEKNNEGPQ VPTIDLKEIE
     SDNEKVRAKC REKLKKAAVD WGVMHLVNHG ISDELMDKVR KAGKAFFDLP IEQKEKYAND
     QASGKIQGYG SKLANNASGQ LEWEDYFFHC VYPEDKRDLS IWPQTPADYI EATAEYAKQL
     RELATKVLKV LSLGLGLDEG RLEKEVGGLE ELLLQMKINY YPKCPQPELA LGVEAHTDVS
     ALTFILHNMV PGLQLFYEGK WVTAKCVPNS IVMHIGDTLE ILSNGKYKSI LHRGMVNKEK
     VRISWAVFCE PPKEKIILKP LPETVSEDEP AMFPPRTFAE HIQHKLFRKS QEALLPK
//