Reviewed, UniProtKB/Swiss-Prot P51091 (LDOX_MALDO)

Names and origin

Protein names

Recommended name:
    Leucoanthocyanidin dioxygenase
      Short name=LDOX
      Short name=Leucocyanidin oxygenase
    EC=1.14.11.19
Alternative name(s):
    Leucoanthocyanidin hydroxylase
    Anthocyanidin synthase

Gene names

Name:

ANS

Organism

Malus domestica (Apple) (Pyrus malus)

Taxonomic identifier

3750 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Rosales › Rosaceae › Maloideae › Malus

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Oxidation of leucoanthocyanidins into anthocyanidins.
Catalytic activity	Leucocyanidin + 2-oxoglutarate + O2 = cis- and trans-dihydroquercetins + succinate + CO2 + H2O.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 ascorbate molecule per subunit By similarity.
Pathway	Pigment biosynthesis; anthocyanin biosynthesis.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW leucocyanidin oxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Leucoanthocyanidin dioxygenase		PRO_0000067301
Regions
	Domain	212 – 311	100	Fe2OG dioxygenase
Sites
	Metal binding	236	1	Iron By similarity
	Metal binding	238	1	Iron By similarity
	Metal binding	292	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51091-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: FA5D97C25267B3E6	FASTA	357	40,332
	10 20 30 40 50 60 MVSSDSVNSR VETLAGSGIS TIPKEYIRPK DELVNIGDIF EQEKNNEGPQ VPTIDLKEIE 70 80 90 100 110 120 SDNEKVRAKC REKLKKAAVD WGVMHLVNHG ISDELMDKVR KAGKAFFDLP IEQKEKYAND 130 140 150 160 170 180 QASGKIQGYG SKLANNASGQ LEWEDYFFHC VYPEDKRDLS IWPQTPADYI EATAEYAKQL 190 200 210 220 230 240 RELATKVLKV LSLGLGLDEG RLEKEVGGLE ELLLQMKINY YPKCPQPELA LGVEAHTDVS 250 260 270 280 290 300 ALTFILHNMV PGLQLFYEGK WVTAKCVPNS IVMHIGDTLE ILSNGKYKSI LHRGMVNKEK 310 320 330 340 350 VRISWAVFCE PPKEKIILKP LPETVSEDEP AMFPPRTFAE HIQHKLFRKS QEALLPK

References

[1]	"A Malus cDNA with homology to the Antirrhinum Candica and Zea A2 genes." Davies K.M. Plant Physiol. 103:1015-1015(1993) [PubMed: 8022923] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.
[2]	"Molecular cloning and expression of anthocyanin biosynthesis genes from 'Fuji apple'." Lee J.-R., Hong S.-T., Yoo Y.G., Kim S.-R. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Fuji. Tissue: Peelings.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71360 mRNA. Translation: CAA50498.1. AF117269 mRNA. Translation: AAD26205.1.
PIR	S33144.
3D structure databases
SMR	P51091. Positions 10-354.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.11.19. 66979.
Family and domain databases
InterPro	IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDOX_MALDO

Accession

Primary (citable) accession number: P51091

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)