P51090 (CHSY_VITVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chalcone synthase
EC=2.3.1.74

Alternative name(s):
Naringenin-chalcone synthase

Gene names

Name:

CHS

Organism

Vitis vinifera (Grape)

Taxonomic identifier

29760 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › rosids incertae sedis › Vitales › Vitaceae › Vitis

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.
Catalytic activity	3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Induction	By light.
Sequence similarities	Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological_process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	naringenin-chalcone synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Chalcone synthase		PRO_0000216075
Sites
	Active site	164	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51090 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C44992BDFF4EE146	FASTA	393	42,701
	10 20 30 40 50 60 MVSVAEIRKA QRAEGPATVL AIGTATPANC VYQADYPDYY FRITNSEHMT ELKEKFKRMC 70 80 90 100 110 120 EKSMINKRYM HLTEEILKEN PNVCAYMAPS LDARQDMVVV EVPKLGKEAA AKAIKEWGQP 130 140 150 160 170 180 KSKITHLVFC TTSGVDMPGA DYQLTKLLGL KPSVKRLMMY QQGCFAGGTV LRLAKDLAEN 190 200 210 220 230 240 NAGSRVLVVC SEITAVTFRG PSDTHLDSLV GQALFGDGAA AVIIGADPDT KIELPLFELV 250 260 270 280 290 300 SAAQTILPDS EGAIDGHLRE VGLTFHLLKD VPGLISKNIE KSLVEAFTPI GISDWNSLFW 310 320 330 340 350 360 IAHPGGPAIL DQVELKLGLK EEKLRATRHV LSEYGNMSSA CVLFILDEMR KKSIEEGKGS 370 380 390 TGEGLEWGVL FGFGPGLTVE TVVLHSVSAP AAH

References

[1]

"Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.)." Sparvoli F., Martin C., Scienza A., Gavazzi G., Tonelli C. Plant Mol. Biol. 24:743-755(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Lambrusco Foglia Frastagliata.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X75969 mRNA. Translation: CAA53583.1.
UniGene	Vvi.117.
3D structure databases
ProteinModelPortal	P51090.
SMR	P51090. Positions 3-388.
ModBase	Search...
Proteomic databases
PRIDE	P51090.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG3424.
Enzyme and pathway databases
UniPathway	UPA00154.
Gene expression databases
ArrayExpress	P51090.
Family and domain databases
Gene3D	3.40.47.10. 2 hits.
InterPro	IPR012328. Chalcone/stilbene_synth_C. IPR018088. Chalcone/stilbene_synthase_AS. IPR001099. Chalcone/stilbene_synthase_N. IPR011141. Polyketide_synthase_type-III. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF02797. Chal_sti_synt_C. 1 hit. PF00195. Chal_sti_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000451. PKS_III. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
PROSITE	PS00441. CHALCONE_SYNTH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHSY_VITVI

Accession

Primary (citable) accession number: P51090

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program