P51080 (CHS7_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chalcone synthase
EC=2.3.1.74

Alternative name(s):
Naringenin-chalcone synthase

Gene names

Name:

CHSII

Organism

Medicago sativa (Alfalfa)

Taxonomic identifier

3879 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	265 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.
Catalytic activity	3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Induction	By infection.
Sequence similarities	Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological_process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	naringenin-chalcone synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 265	›265	Chalcone synthase		PRO_0000216012
Sites
	Active site	40	1	By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P51080 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 8A4659A0DF564A1F	FASTA	265	28,621
	10 20 30 40 50 60 ITHLIVCTTS VDMPGADYQL TKLLGLRPYV KRYMMYQQGC FAGGTVLRLA KDLAENNKGA 70 80 90 100 110 120 RVLVVCSEVT AVTFRGPSDT HLDSLVGQAL FGDGAAALIV GSDPVPEIEK PIFEMVWTAQ 130 140 150 160 170 180 TIAPDSEGAI DGHLREAGLT FHLLKDVPGI VSKNINKALV EAFEPLGISD YNSIFWIAHP 190 200 210 220 230 240 GGPAILDQVE QKLALKPEKM KATREVLSEY GNMSSACVLF ILDEMRKKSA QDGLKTTGEG 250 260 LEWGVLFGFG PGLTIETVVL RSVTI

References

[1]

"Pathological and molecular characterizations of alfalfa interactions with compatible and incompatible bacteria, Xanthomonas campestris pv. alfalfae and Pseudomonas syringae pv. pisi." Esnault R., Buffard D., Breda C., Sallaud C., El-Turk J., Kondorosi A. Mol. Plant Microbe Interact. 6:655-664(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nagyszenasi. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68107 mRNA. Translation: CAA48227.1.
PIR	S26415.
3D structure databases
ProteinModelPortal	P51080.
SMR	P51080. Positions 1-265.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00154.
Family and domain databases
Gene3D	3.40.47.10. 2 hits.
InterPro	IPR012328. Chalcone/stilbene_synth_C. IPR018088. Chalcone/stilbene_synthase_AS. IPR001099. Chalcone/stilbene_synthase_N. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF02797. Chal_sti_synt_C. 1 hit. PF00195. Chal_sti_synt_N. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 2 hits.
PROSITE	PS00441. CHALCONE_SYNTH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHS7_MEDSA

Accession

Primary (citable) accession number: P51080

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program