P51078 (CHS5_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chalcone synthase 4-2
EC=2.3.1.74

Alternative name(s):
Naringenin-chalcone synthase 4-2

Gene names

Name:	CHS4-2
Synonyms:	CHSI

Organism

Medicago sativa (Alfalfa)

Taxonomic identifier

3879 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.
Catalytic activity	3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Developmental stage	Highest expression in young root tips.
Induction	By infection.
Sequence similarities	Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological_process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	naringenin-chalcone synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	Chalcone synthase 4-2		PRO_0000216010
Sites
	Active site	164	1	By similarity
Experimental info
	Sequence conflict	86	1	I → Y in AAB41560. Ref.2
	Sequence conflict	94	1	W → R in AAB41560. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P51078 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B33674EC7709F170	FASTA	389	42,661
	10 20 30 40 50 60 MVSVSEIRNA QRAEGPATTL AIGTANPTNC VEQSTYPDFY FKITNSEHKT ELKEKFQRMC 70 80 90 100 110 120 DKSMIKRRYM YLTEEILKEN PSVCEIMAPS LDAWQDMVVV EVPRLGKEAA VKAIKEWGQP 130 140 150 160 170 180 KSKITHLIVC TTSGVDMPGA DYQLTKLLGL RPYVKRYMMY QQGCFAGGTV LRLAKDLAEN 190 200 210 220 230 240 NKGARVLVVC SEVTAVTFRG PSDTHLDSLV GQALFGDGAA ALIVGSDPVP EIEKPIFEMV 250 260 270 280 290 300 WTAQTIAPDS EGAIDGHLRE AGLTFHLLKD VPGIVSKNIN KALVEAFEPL GISDYNSIFW 310 320 330 340 350 360 IAHPGGPAIL DQVEQKLALK PEKMKATREV LSEYGNMSSA CVLFILDEMR KKSAQDGLKT 370 380 TGEGLEWGVL FGFGPGLTIE TVVLRSVAI

References

[1]

"Pathological and molecular characterizations of alfalfa interactions with compatible and incompatible bacteria, Xanthomonas campestris pv. alfalfae and Pseudomonas syringae pv. pisi." Esnault R., Buffard D., Breda C., Sallaud C., El-Turk J., Kondorosi A. Mol. Plant Microbe Interact. 6:655-664(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nagyszenasi. Tissue: Leaf.

[2]

"Isolation of chalcone synthase and chalcone isomerase cDNAs from alfalfa (Medicago sativa L.): highest transcript levels occur in young roots and root tips." McKhann H.I., Hirsch A.M. Plant Mol. Biol. 24:767-777(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-389. Strain: cv. Iroquois. Tissue: Root nodule.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68106 mRNA. Translation: CAA48226.1. U01019 mRNA. Translation: AAB41560.1.
PIR	S26414.
3D structure databases
ProteinModelPortal	P51078.
SMR	P51078. Positions 3-389.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00154.
Family and domain databases
Gene3D	3.40.47.10. 2 hits.
InterPro	IPR012328. Chalcone/stilbene_synth_C. IPR018088. Chalcone/stilbene_synthase_AS. IPR001099. Chalcone/stilbene_synthase_N. IPR011141. Polyketide_synthase_type-III. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF02797. Chal_sti_synt_C. 1 hit. PF00195. Chal_sti_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000451. PKS_III. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
PROSITE	PS00441. CHALCONE_SYNTH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHS5_MEDSA

Accession

Primary (citable) accession number: P51078

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program