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P51078 (CHS5_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chalcone synthase 4-2

EC=2.3.1.74
Alternative name(s):
Naringenin-chalcone synthase 4-2
Gene names
Name:CHS4-2
Synonyms:CHSI
OrganismMedicago sativa (Alfalfa)
Taxonomic identifier3879 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Protein attributes

Sequence length389 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

Catalytic activity

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Developmental stage

Highest expression in young root tips.

Induction

By infection.

Sequence similarities

Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
   Biological processFlavonoid biosynthesis
   Molecular functionAcyltransferase
Transferase
Gene Ontology (GO)
   Biological_processflavonoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionnaringenin-chalcone synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 389389Chalcone synthase 4-2
PRO_0000216010

Sites

Active site1641 By similarity

Experimental info

Sequence conflict861I → Y in AAB41560. Ref.2
Sequence conflict941W → R in AAB41560. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P51078 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: B33674EC7709F170

FASTA38942,661
        10         20         30         40         50         60 
MVSVSEIRNA QRAEGPATTL AIGTANPTNC VEQSTYPDFY FKITNSEHKT ELKEKFQRMC 

        70         80         90        100        110        120 
DKSMIKRRYM YLTEEILKEN PSVCEIMAPS LDAWQDMVVV EVPRLGKEAA VKAIKEWGQP 

       130        140        150        160        170        180 
KSKITHLIVC TTSGVDMPGA DYQLTKLLGL RPYVKRYMMY QQGCFAGGTV LRLAKDLAEN 

       190        200        210        220        230        240 
NKGARVLVVC SEVTAVTFRG PSDTHLDSLV GQALFGDGAA ALIVGSDPVP EIEKPIFEMV 

       250        260        270        280        290        300 
WTAQTIAPDS EGAIDGHLRE AGLTFHLLKD VPGIVSKNIN KALVEAFEPL GISDYNSIFW 

       310        320        330        340        350        360 
IAHPGGPAIL DQVEQKLALK PEKMKATREV LSEYGNMSSA CVLFILDEMR KKSAQDGLKT 

       370        380 
TGEGLEWGVL FGFGPGLTIE TVVLRSVAI 

« Hide

References

[1]"Pathological and molecular characterizations of alfalfa interactions with compatible and incompatible bacteria, Xanthomonas campestris pv. alfalfae and Pseudomonas syringae pv. pisi."
Esnault R., Buffard D., Breda C., Sallaud C., El-Turk J., Kondorosi A.
Mol. Plant Microbe Interact. 6:655-664(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Nagyszenasi.
Tissue: Leaf.
[2]"Isolation of chalcone synthase and chalcone isomerase cDNAs from alfalfa (Medicago sativa L.): highest transcript levels occur in young roots and root tips."
McKhann H.I., Hirsch A.M.
Plant Mol. Biol. 24:767-777(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-389.
Strain: cv. Iroquois.
Tissue: Root nodule.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X68106 mRNA. Translation: CAA48226.1.
U01019 mRNA. Translation: AAB41560.1.
PIRS26414.

3D structure databases

ProteinModelPortalP51078.
SMRP51078. Positions 3-389.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00154.

Family and domain databases

Gene3D3.40.47.10. 2 hits.
InterProIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFPIRSF000451. PKS_III. 1 hit.
SUPFAMSSF53901. SSF53901. 2 hits.
PROSITEPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHS5_MEDSA
AccessionPrimary (citable) accession number: P51078
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways