ID   CHSY_FRAAN              Reviewed;          99 AA.
AC   P51076;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   27-MAR-2024, entry version 86.
DE   RecName: Full=Chalcone synthase RJ5;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase;
DE   Flags: Fragment;
OS   Fragaria ananassa (Strawberry) (Fragaria chiloensis x Fragaria virginiana).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Rosaceae; Rosoideae; Potentilleae; Fragariinae;
OC   Fragaria.
OX   NCBI_TaxID=3747;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Pajaro; TISSUE=Fruit;
RX   PubMed=7766892; DOI=10.1007/bf00020883;
RA   Wilkinson J.Q., Lanahan M.B., Conner T.W., Klee H.J.;
RT   "Identification of mRNAs with enhanced expression in ripening strawberry
RT   fruit using polymerase chain reaction differential display.";
RL   Plant Mol. Biol. 27:1097-1108(1995).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- TISSUE SPECIFICITY: Predominantly in developing fruit.
CC   -!- DEVELOPMENTAL STAGE: Expression of the protein is enhanced in a
CC       ripening fruit.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; U19942; AAA79923.1; -; mRNA.
DR   PIR; S56675; S56675.
DR   AlphaFoldDB; P51076; -.
DR   SMR; P51076; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 1.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877:SF14; CHALCONE SYNTHASE; 1.
DR   PANTHER; PTHR11877; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   SUPFAM; SSF53901; Thiolase-like; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           <1..99
FT                   /note="Chalcone synthase RJ5"
FT                   /id="PRO_0000215976"
FT   NON_TER         1
SQ   SEQUENCE   99 AA;  10715 MW;  38F83B45F6B1179B CRC64;
     NITDWNSLFW IAHPGGPAIL DQVEAKLALK PEKLEATRHI LSEYGNMSSA CVLFILDEVR
     RKSAANGHKT TGEGLEWGVL FGFGPGLTVE TVVLHSVSA
//