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P51066 (ACEA_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isocitrate lyase
Short name=ICL
Short name=Isocitrase
Short name=Isocitratase
EC=4.1.3.1
Gene names
Name:aceA
Ordered Locus Names:STM4184
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella ›

Protein attributes

Sequence length434 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase-positive serine pathway By similarity.

Catalytic activity

Isocitrate = succinate + glyoxylate.

Cofactor

Divalent cations.

Enzyme regulation

Is activated by phosphorylation (on histidine) and is inhibited by PEP, 3-phosphoglycerate and succinate By similarity.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentCytoplasm
   Molecular functionLyase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionisocitrate lyase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 434434Isocitrate lyase
PRO_0000068776

Sites

Active site1951 By similarity

Natural variations

Natural variant3901A → G in strain: S3041.
Natural variant3921D → E in strain: S3013, S3014, S3015, S3027 and S3041.
Natural variant4321A → S in strain: S2978, S2979, S2980, S2983, S2985, S2993, S2995, S3013, S3014, S3015, S3027, S3041, S3044, S3057 and S3333.

Sequences

Sequence LengthMass (Da)Tools
P51066 [UniParc].

Last modified December 19, 2001. Version 2.
Checksum: 4ADC46FB4A4A5C60

FASTA43447,563
        10         20         30         40         50         60 
MKTRTQQIEE LQKEWTQPRW EGITRPYSAE EVVKLRGSVN PECTLAQLGA AKMWRLLHGE 

        70         80         90        100        110        120 
AKKGYINSLG ALTGGQALQQ AKAGIEAIYL SGWQVAADAN LASSMYPDQS LYPANSVPAV 

       130        140        150        160        170        180 
VDRINNTFRR ADQIQWASGI EPNDPRYVDY FLPIVADAEA GFGGVLNAFE LMKSMIEAGA 

       190        200        210        220        230        240 
AAVHFEDQLA SVKKCGHMGG KVLVPTQEAI QKLVAARLAA DVMGVPTLVI ARTDADAADL 

       250        260        270        280        290        300 
ITSDCDPYDS GFITGERTSE GFYRTHAGIE QAISRGLAYA PYADLVWCET STPDLELARR 

       310        320        330        340        350        360 
FADAIHAKYP GKLLAYNCSP SFNWQKNLDD KTIASFQQQL SDMGYKYQFI TLAGIHSMWF 

       370        380        390        400        410        420 
NMFDLAHAYA QGEGMKHYVE KVQQPEFAAA KDGYTFVSHQ QEVGTGYFDK VTTIIQGGAS 

       430 
SVTALTGSTE EAQF

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.
[2]"Size and sequence polymorphism in the isocitrate dehydrogenase kinase/phosphatase gene (aceK) and flanking regions in Salmonella enterica and Escherichia coli."
Nelson K., Wang F.S., Boyd E.F., Selander R.K.
Genetics 147:1509-1520(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 365-434.
Strain: S2978, S2979, S2980, S2983, S2985, S2993, S2995, S3013, S3014, S3015, S3027, S3041, S3044, S3057, S3333 and S4194.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE006468 Genomic DNA. Translation: AAL23008.1.
U43344 Genomic DNA. Translation: AAC43867.1.
U43345 Genomic DNA. Translation: AAC43869.1.
U43346 Genomic DNA. Translation: AAC43871.1.
U43347 Genomic DNA. Translation: AAC43872.1.
U43348 Genomic DNA. Translation: AAC43874.1.
U43349 Genomic DNA. Translation: AAC43876.1.
U43350 Genomic DNA. Translation: AAC43878.1.
U43351 Genomic DNA. Translation: AAC43880.1.
U43352 Genomic DNA. Translation: AAC43882.1.
U43353 Genomic DNA. Translation: AAC43884.1.
U43354 Genomic DNA. Translation: AAC43886.1.
U43355 Genomic DNA. Translation: AAC43888.1.
U43356 Genomic DNA. Translation: AAC43890.1.
U43357 Genomic DNA. Translation: AAC43892.1.
U43358 Genomic DNA. Translation: AAC43894.1.
U43359 Genomic DNA. Translation: AAC43896.1.
RefSeqNP_463049.1. NC_003197.1.

3D structure databases

ProteinModelPortalP51066.
SMRP51066. Positions 2-417.
ModBaseSearch...

Protein-protein interaction databases

STRING99287.STM4184.

Proteomic databases

PaxDbP51066.
PRIDEP51066.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL23008; AAL23008; STM4184.
GeneID1255710.
KEGGstm:STM4184.
PATRIC32387257. VBISalEnt20916_4396.

Phylogenomic databases

eggNOGCOG2224.
HOGENOMHOG000238475.
KOK01637.
OMAQAVQQVK.
ProtClustDBPRK15063.

Enzyme and pathway databases

UniPathwayUPA00703; UER00719.

Family and domain databases

Gene3D3.20.20.60. 1 hit.
InterProIPR006254. Isocitrate_lyase.
IPR000918. Isocitrate_lyase/Pmutase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase.
[Graphical view]
PANTHERPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamPF00463. ICL. 1 hit.
[Graphical view]
PIRSFPIRSF001362. Isocit_lyase. 1 hit.
SUPFAMSSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMsTIGR01346. isocit_lyase. 2 hits.
PROSITEPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACEA_SALTY
AccessionPrimary (citable) accession number: P51066
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 19, 2001
Last modified: May 1, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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