Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isocitrate lyase

Gene

aceA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the metabolic adaptation in response to environmental changes. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity

Cofactori

Mg2+By similarity

Pathway: glyoxylate cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (aceA)
  2. Malate synthase (aceB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi157 – 1571MagnesiumBy similarity
Active sitei195 – 1951Proton acceptorBy similarity
Binding sitei232 – 2321SubstrateBy similarity
Binding sitei351 – 3511SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-4214-MONOMER.
UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity)
Short name:
ICLBy similarity
Alternative name(s):
IsocitraseBy similarity
IsocitrataseBy similarity
Gene namesi
Name:aceA
Ordered Locus Names:STM4184
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 434434Isocitrate lyasePRO_0000068776Add
BLAST

Proteomic databases

PaxDbiP51066.
PRIDEiP51066.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi99287.STM4184.

Structurei

3D structure databases

ProteinModelPortaliP51066.
SMRiP51066. Positions 2-417.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni91 – 933Substrate bindingBy similarity
Regioni196 – 1972Substrate bindingBy similarity
Regioni317 – 3215Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2224.
HOGENOMiHOG000238475.
KOiK01637.
OMAiRWKGVTR.
OrthoDBiEOG689HMX.
PhylomeDBiP51066.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 2 hits.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTRTQQIEE LQKEWTQPRW EGITRPYSAE EVVKLRGSVN PECTLAQLGA
60 70 80 90 100
AKMWRLLHGE AKKGYINSLG ALTGGQALQQ AKAGIEAIYL SGWQVAADAN
110 120 130 140 150
LASSMYPDQS LYPANSVPAV VDRINNTFRR ADQIQWASGI EPNDPRYVDY
160 170 180 190 200
FLPIVADAEA GFGGVLNAFE LMKSMIEAGA AAVHFEDQLA SVKKCGHMGG
210 220 230 240 250
KVLVPTQEAI QKLVAARLAA DVMGVPTLVI ARTDADAADL ITSDCDPYDS
260 270 280 290 300
GFITGERTSE GFYRTHAGIE QAISRGLAYA PYADLVWCET STPDLELARR
310 320 330 340 350
FADAIHAKYP GKLLAYNCSP SFNWQKNLDD KTIASFQQQL SDMGYKYQFI
360 370 380 390 400
TLAGIHSMWF NMFDLAHAYA QGEGMKHYVE KVQQPEFAAA KDGYTFVSHQ
410 420 430
QEVGTGYFDK VTTIIQGGAS SVTALTGSTE EAQF
Length:434
Mass (Da):47,563
Last modified:December 19, 2001 - v2
Checksum:i4ADC46FB4A4A5C60
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti390 – 3901A → G in strain: S3041.
Natural varianti392 – 3921D → E in strain: S3013, S3014, S3015, S3027 and S3041.
Natural varianti432 – 4321A → S in strain: S2978, S2979, S2980, S2983, S2985, S2993, S2995, S3013, S3014, S3015, S3027, S3041, S3044, S3057 and S3333.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL23008.1.
U43344 Genomic DNA. Translation: AAC43867.1.
U43345 Genomic DNA. Translation: AAC43869.1.
U43346 Genomic DNA. Translation: AAC43871.1.
U43347 Genomic DNA. Translation: AAC43872.1.
U43348 Genomic DNA. Translation: AAC43874.1.
U43349 Genomic DNA. Translation: AAC43876.1.
U43350 Genomic DNA. Translation: AAC43878.1.
U43351 Genomic DNA. Translation: AAC43880.1.
U43352 Genomic DNA. Translation: AAC43882.1.
U43353 Genomic DNA. Translation: AAC43884.1.
U43354 Genomic DNA. Translation: AAC43886.1.
U43355 Genomic DNA. Translation: AAC43888.1.
U43356 Genomic DNA. Translation: AAC43890.1.
U43357 Genomic DNA. Translation: AAC43892.1.
U43358 Genomic DNA. Translation: AAC43894.1.
U43359 Genomic DNA. Translation: AAC43896.1.
RefSeqiNP_463049.1. NC_003197.1.
WP_000857881.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL23008; AAL23008; STM4184.
GeneIDi1255710.
KEGGistm:STM4184.
PATRICi32387257. VBISalEnt20916_4396.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL23008.1.
U43344 Genomic DNA. Translation: AAC43867.1.
U43345 Genomic DNA. Translation: AAC43869.1.
U43346 Genomic DNA. Translation: AAC43871.1.
U43347 Genomic DNA. Translation: AAC43872.1.
U43348 Genomic DNA. Translation: AAC43874.1.
U43349 Genomic DNA. Translation: AAC43876.1.
U43350 Genomic DNA. Translation: AAC43878.1.
U43351 Genomic DNA. Translation: AAC43880.1.
U43352 Genomic DNA. Translation: AAC43882.1.
U43353 Genomic DNA. Translation: AAC43884.1.
U43354 Genomic DNA. Translation: AAC43886.1.
U43355 Genomic DNA. Translation: AAC43888.1.
U43356 Genomic DNA. Translation: AAC43890.1.
U43357 Genomic DNA. Translation: AAC43892.1.
U43358 Genomic DNA. Translation: AAC43894.1.
U43359 Genomic DNA. Translation: AAC43896.1.
RefSeqiNP_463049.1. NC_003197.1.
WP_000857881.1. NC_003197.1.

3D structure databases

ProteinModelPortaliP51066.
SMRiP51066. Positions 2-417.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM4184.

Proteomic databases

PaxDbiP51066.
PRIDEiP51066.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL23008; AAL23008; STM4184.
GeneIDi1255710.
KEGGistm:STM4184.
PATRICi32387257. VBISalEnt20916_4396.

Phylogenomic databases

eggNOGiCOG2224.
HOGENOMiHOG000238475.
KOiK01637.
OMAiRWKGVTR.
OrthoDBiEOG689HMX.
PhylomeDBiP51066.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.
BioCyciSENT99287:GCTI-4214-MONOMER.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 2 hits.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  2. "Size and sequence polymorphism in the isocitrate dehydrogenase kinase/phosphatase gene (aceK) and flanking regions in Salmonella enterica and Escherichia coli."
    Nelson K., Wang F.S., Boyd E.F., Selander R.K.
    Genetics 147:1509-1520(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 365-434.
    Strain: S2978, S2979, S2980, S2983, S2985, S2993, S2995, S3013, S3014, S3015, S3027, S3041, S3044, S3057, S3333 and S4194.

Entry informationi

Entry nameiACEA_SALTY
AccessioniPrimary (citable) accession number: P51066
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 19, 2001
Last modified: May 27, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.