P51064 (IPYR_BARBK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	ppa
Ordered Locus Names:	BARBAKC583_0329

Organism

Bartonella bacilliformis (strain ATCC 35685 / KC583) [Complete proteome] [HAMAP]

Taxonomic identifier

360095 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm HAMAP MF_00209.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Inorganic pyrophosphatase HAMAP MF_00209		PRO_0000137480
Sites
	Metal binding	66	1	Magnesium 1 By similarity
	Metal binding	71	1	Magnesium 1 By similarity
	Metal binding	71	1	Magnesium 2 By similarity
	Metal binding	103	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51064 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: E327E6637386DD65	FASTA	177	20,064
	10 20 30 40 50 60 MNIKEIPIGN NPPEDVNVII EVPVDSQPVK YEMDKKSGSL FVDRFISTSM VYPGNYGFIP 70 80 90 100 110 120 HTLSEDSDPI DVLVCNTRPL IPGCVINVRP IGALIMEDDG GKDEKIIAVP TPKLTQQYIG 130 140 150 160 170 IHDYTDLTEN ILKKIEHFFK HYKDLEAGKW AKIEGWRDKN FARELIQQAI ERAKAIQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, functional expression, and complementation analysis of an inorganic pyrophosphatase from Bartonella bacilliformis." Mitchell S.J., Minnick M.F. Can. J. Microbiol. 43:734-743(1997) [PubMed: 9304784] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Hendrix L., Mohamoud Y., Radune D., Shvartsbeyn A., Daugherty S., Dodson R., Durkin A.S., Harkins D., Huot H., Kothari S.P., Madupu R., Li J., Nelson W.C., Shrivastava S., Giglio M.G., Haft D., Selengut J., Fraser-Ligget C., Seshadri R. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35685 / KC583.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L46591 Genomic DNA. Translation: AAB88054.1. CP000524 Genomic DNA. Translation: ABM44595.1.
RefSeq	YP_988649.1. NC_008783.1.
3D structure databases
ProteinModelPortal	P51064.
SMR	P51064. Positions 3-172.
ModBase	Search...
Protein-protein interaction databases
STRING	P51064.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4684697.
GenomeReviews	Gene locus BARBAKC583_0329 in contig CP000524_GR.
KEGG	bbk:BARBAKC583_0329.
NMPDR	fig|360095.3.peg.380.
PATRIC	20536927. VBIBarBac6912_0313.
TIGR	BARBAKC583_0329.
Phylogenomic databases
eggNOG	COG0221.
HOGENOM	HBG529150.
OMA	PNDFNVI.
ProtClustDB	PRK01250.
Enzyme and pathway databases
BioCyc	BBAC360095:BARBAKC583_0329-MONOMER.
Family and domain databases
HAMAP	MF_00209. Inorganic_PPase. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K01507.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_BARBK

Accession

Primary (citable) accession number: P51064
Secondary accession number(s): A1URP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program