P51059 (CAPP2_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxylase 2
Short name=PEPC 2
Short name=PEPCase 2
EC=4.1.1.31

Gene names

Name:	PEP4
Synonyms:	PEP

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	967 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.
Catalytic activity	Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.
Cofactor	Magnesium By similarity.
Enzyme regulation	By light-reversible phosphorylation By similarity.
Pathway	Photosynthesis; C3 acid pathway.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PEPCase type 1 family.

Ontologies

Keywords
Biological process	Carbon dioxide fixation Photosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium
Molecular function	Lyase
PTM	Phosphoprotein
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological_process	carbon fixation Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Cellular_component	apoplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene cytosol Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	phosphoenolpyruvate carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 967	967	Phosphoenolpyruvate carboxylase 2		PRO_0000166668
Sites
	Active site	174	1	By similarity
	Active site	602	1	By similarity
Amino acid modifications
	Modified residue	13	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51059 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 7034A2AD5521645B	FASTA	967	109,999
	10 20 30 40 50 60 MAALGPKMER LSSIDAQLRM LVPGKVSEDD KLIEYDALLL DRFLDILQDL HGDDLKEMVQ 70 80 90 100 110 120 ECYEVAAEYE TKHDLQKLDE LGKMITSLDP GDSIVIAKSL SHMLNLANLA EEVQIAYRRR 130 140 150 160 170 180 IKLKKGDFAD ENSAITESDI EETLKRLVVD LKKSPAEVFD ALKSQTVDLV LTAHPTQSVR 190 200 210 220 230 240 RSLLQKHSRI RNCLVQLYSK DITPDDKQEL DEALQREIQA AFRTDEIRRT QPTPQDEMRA 250 260 270 280 290 300 GMSYFHETIW KGVPKFLRRV DTALKNIGIN ERVPYNAPLI QFSSWMGGDR DGNPRVTPEV 310 320 330 340 350 360 TRDVCLLARM MASNLYCSQI EDLMFELSMW RCSDELRMRA DVLHLSTKKD AKHYIEFWKK 370 380 390 400 410 420 VPPNEPYRVI LSDVRDKLYN TRERSRELLS SGHSDIPEEA TLTNVEQLLE PLELCYRSLC 430 440 450 460 470 480 ACGDSVIADG TLLDFLRQVS TFGLSLVRLD IRQESDRHTD VLDAITTYLG IGSYREWTEE 490 500 510 520 530 540 RRQEWLLSEL NGKRPLFGSD LPKTEEISDV LDTFHVIAEL PSDNFGAYII SMATAPSDVL 550 560 570 580 590 600 AVELLQRECH VKTPLRVVPL FEKLADLEAA PAALARLFSI DWYRQRINGK QEVMIGYSDS 610 620 630 640 650 660 GKDAGRLSAA WQLYKAQEEL IKVAKDFGVK LTMFHGRGGT VGRGGGPTHL AILSQPPDTI 670 680 690 700 710 720 HGSLRVTVQG EVIEQSFGEE HLCFRTLQRF TAATLEHGMH PPNAPKPEWR ALLDEMAVVA 730 740 750 760 770 780 TEEYRSIVFK EPRFVEYFRL ATPETEYGRM NIGSRPSKRK PSGGIDSLRA IPWIFAWTQT 790 800 810 820 830 840 RFHLPVWLGF GAAFKNVLQK DIRNLHMLQE MYNEWPFFRV TIDLVEMVFA KGNPGIAALY 850 860 870 880 890 900 DKLLVSEELH PLGEKLRANY EETQKLLLQV AGHRDLLEGD LYLKQRLRLR DAYITTLNVC 910 920 930 940 950 960 QAYTLKRIRD PDYHVALRPH LSKEIMDSTK AAADVVKLNP GSEYAPGLED TLILTMKGIA AGLQNTG

References

[1]

"Molecular evolution of phosphoenolpyruvate carboxylase for C4 photosynthesis in maize: comparison of its cDNA sequence with a newly isolated cDNA encoding an isozyme involved in the anaplerotic function." Kawamura T., Shigesada K., Toh H., Okumura S., Yanagisawa S., Izui K. J. Biochem. 112:147-154(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. H84. Tissue: Root.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61489 mRNA. Translation: CAA43709.1.
PIR	JH0667.
RefSeq	NP_001105438.1. NM_001111968.1.
UniGene	Zm.163236.
3D structure databases
ProteinModelPortal	P51059.
SMR	P51059. Positions 33-967.
ModBase	Search...
Proteomic databases
PRIDE	P51059.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	542393.
KEGG	zma:542393.
Organism-specific databases
Gramene	P51059.
MaizeGDB	30066.
Phylogenomic databases
HOGENOM	HOG000238648.
KO	K01595.
Enzyme and pathway databases
SABIO-RK	P51059.
UniPathway	UPA00321.
Family and domain databases
InterPro	IPR021135. PEP_COase. IPR018129. PEP_COase_AS. IPR022805. PEP_COase_bac/pln-type. IPR015813. Pyrv/PenolPyrv_Kinase-like_dom. [Graphical view]
Pfam	PF00311. PEPcase. 1 hit. [Graphical view]
PRINTS	PR00150. PEPCARBXLASE.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PROSITE	PS00781. PEPCASE_1. 1 hit. PS00393. PEPCASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAPP2_MAIZE

Accession

Primary (citable) accession number: P51059

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program