P51056 (ODO1_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-oxoglutarate dehydrogenase E1 component
EC=1.2.4.2

Alternative name(s):
Alpha-ketoglutarate dehydrogenase

Gene names

Name:	sucA
Ordered Locus Names:	CBU_1399

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	934 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
Catalytic activity	2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2.
Cofactor	Thiamine pyrophosphate.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the alpha-ketoglutarate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular_function	oxoglutarate dehydrogenase (succinyl-transferring) activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 934	934	2-oxoglutarate dehydrogenase E1 component		PRO_0000162190
Experimental info
	Sequence conflict	600	1	A → R in AAA61785. Ref.1
	Sequence conflict	614 – 630	17	VGQDS…RHAVV → SVKIPDEALFFIAMPLW in AAA61785. Ref.1
	Sequence conflict	778 – 782	5	PKSVL → QKCA in AAA61785. Ref.1
	Sequence conflict	809 – 816	8	HDPKKITR → RIRKKSPA in AAA61785. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P51056 [UniParc]. Last modified May 9, 2003. Version 3. Checksum: 7FB8144F8E013E8D	FASTA	934	106,723
	10 20 30 40 50 60 MPKITMQQFQ KNSYLADNNA GYIETLYENF LKDPHSVNEE WRSYFRTLTN GASTPDISHA 70 80 90 100 110 120 TIREEFRELA RKPRSISPTA ITPAAEQAAV DLLIEGYRRF GHLNAKINPL GDNRPVDSRL 130 140 150 160 170 180 ELGHYNLTES DFNKTFATYG LLNKPKATLK EIYTRLREIY CGSIGVQYST ISDERERNWL 190 200 210 220 230 240 RDYVEQRLPS IEFDKETKRN ILQQLVTAES LEKYLDTKYV GQVRYSLEGG DSLIPLLDEL 250 260 270 280 290 300 TKRARHQKIE EIVICMAHRG RVNVLLNIMG QSAAELFQEF EGKKDYGLMS GDVKYHRGYS 310 320 330 340 350 360 RDVKTDAGPI HLSLAFNPSH LEFICPVAMG SVRARQERQN GHKRDYAMTV MIHGDASFSG 370 380 390 400 410 420 EGIVMEALSM SQTRAHHVGG SIHIILNNQV GFTTSNPHDA RSSMYCSDIA KMLDAPVFHV 430 440 450 460 470 480 NGDDPEAVVA VTQLALDYRM AFHKDVFIDL VCYRRHGHQE VDDPMPTQPA MYKVIQEHPT 490 500 510 520 530 540 TRTLYAKNLI EKKLCTAEEV DQWIDDYRDR LDRGRQLVET LPEGLSAHYA ANWTPYLGQD 550 560 570 580 590 600 WTTLVDTTLP LKKLKALGKK FSTLPNTLHL HRKVEAIYKA RLEMAEGKTP MDWGFAEMLA 610 620 630 640 650 660 YASLLEEGFS VRLVGQDSRR GTFFHRHAVV FDQETGKEYE PLKHLSDKQA APHIYDSLLC 670 680 690 700 710 720 EAGALGFEYG YSTADPNSLV IWEAQFGDFA NVAQVIVDQF ISSGWQKWNR LSGIVLFLPH 730 740 750 760 770 780 GYEGKGPEHS SARLERYLQL CAQNNMQVCA PTTPSQIFHL LRRQVLRPYR KPLVVLTPKS 790 800 810 820 830 840 VLRNKLAVSS LEDLARGQLK LLIPEIEKHD PKKITRVILC SGKVYYDLLA KRREHKGKLN 850 860 870 880 890 900 HIAMIRIEQL YPFPYDELKA ELEKYPNAKQ VIWCQEEPKN QGAWFCTRHR LIKCMRDDQT 910 920 930 LEYVGRSAFA APAAGYSALY VKLQEQLVNQ ALEI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Schimmels J.A., Mallavia L.P. Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Nine Mile.
[2]	Thiele D., Willems H., Oswald W., Krauss H. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Nine Mile phase I.
[3]	"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.
[4]	"Characterization of the succinate dehydrogenase-encoding gene cluster (sdh) from the rickettsia Coxiella burnetii." Heinzen R.A., Mo Y.-Y., Robertson S.J., Mallavia L.P. Gene 155:27-34(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-280. Strain: Nine Mile.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U07789 Unassigned DNA. Translation: AAA61785.1. X77919 Genomic DNA. Translation: CAA54874.1. AE016828 Genomic DNA. Translation: AAO90898.1. L33409 Genomic DNA. Translation: AAA74135.1.
PIR	S42874.
RefSeq	NP_820384.1. NC_002971.3.
3D structure databases
ProteinModelPortal	P51056.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_1399.
Proteomic databases
PRIDE	P51056.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO90898; AAO90898; CBU_1399.
GeneID	1209305.
KEGG	cbu:CBU_1399.
PATRIC	17931555. VBICoxBur82552_1401.
Phylogenomic databases
eggNOG	COG0567.
HOGENOM	HOG000259588.
KO	K00164.
OMA	IIKRGGA.
ProtClustDB	PRK09404.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-1387-MONOMER.
Family and domain databases
InterPro	IPR011603. 2oxoglutarate_DH_E1. IPR001017. DH_E1. IPR005475. Transketolase-like_Pyr-bd. [Graphical view]
PANTHER	PTHR23152. PTHR23152. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. [Graphical view]
PIRSF	PIRSF000157. Oxoglu_dh_E1. 1 hit.
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00239. 2oxo_dh_E1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ODO1_COXBU

Accession

Primary (citable) accession number: P51056

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 9, 2003
Last modified:	May 1, 2013
This is version 91 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program