Reviewed, UniProtKB/Swiss-Prot P51054 (DHSA_COXBU)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase flavoprotein subunit
    EC=1.3.99.1

Gene names

Name:	sdhA
Ordered Locus Names:	CBU_1401

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	587 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur protein, cytochrome b-556 and a hydrophobic protein.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic sideBy similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro succinate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 587	587	Succinate dehydrogenase flavoprotein subunit		PRO_0000158651
Regions
	Nucleotide binding	39 – 54	16	FAD By similarity
Sites
	Active site	288	1	Proton acceptor By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	256	1	Substrate By similarity
	Binding site	355	1	Substrate By similarity
	Binding site	400	1	Substrate By similarity
Amino acid modifications
	Modified residue	47	1	Tele-8alpha-FAD histidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51054-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 37B264ECC98D3DB6	FASTA	587	65,438
	10 20 30 40 50 60 MSSIRVKQYD ALIVGAGGAG LRAALEMAQS RQYKVAVVSK VFPTRSHTVS AQGGIAAALG 70 80 90 100 110 120 NVVPDKPIWH MFDTVKGSDY LGDQDAIQYM CEQAPPSVYE LEHYGLPFSR LDDGRIYQRA 130 140 150 160 170 180 FGGHTRDFGK EMARRTCACA DRTGHAMLHT LYQKNVEAGT HFYYEWYGID LVRGAQGGIA 190 200 210 220 230 240 GMIAMNMETS ELVFFKSRAT IFATGGAGRI YETTSNAYTN TGDGIGMVLR AGLPVQDMEF 250 260 270 280 290 300 WQFHPTGIYG VGCLITEGAR GEGGYLINKD GERFMERYSP HLKDLDCRDV VARSILQEVM 310 320 330 340 350 360 AGGGVGPKKD HVLLKLDHLG EKVLRERLPG IIELSEKFAN VDITKEPIPI LPTCHYMMGG 370 380 390 400 410 420 IPTNIHGQAL TVDENGKDQI IEGLFAAGEC ACVSVHGANR LGTNSLLDLV VFGRAIGLHL 430 440 450 460 470 480 EEALKTELKH RSENPDDIDA AIARLKRWEK PNNVENPALL RQEMRKAMSE DFGVFREEQK 490 500 510 520 530 540 MKQGLERLQK LNERLQRAKL TDTSRTFNNA RIEALELDNL MEVSYATAVS AQQRTESRGA 550 560 570 580 HSRYDYKERD DANWLKHTVY FRDGHIAYRP VNMKPKGMDP FPPKSRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the succinate dehydrogenase-encoding gene cluster (sdh) from the rickettsia Coxiella burnetii." Heinzen R.A., Mo Y.-Y., Robertson S.J., Mallavia L.P. Gene 155:27-34(1995) [PubMed: 7698664] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Nine Mile.
[2]	"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L33409 Genomic DNA. Translation: AAA74133.1. AE016828 Genomic DNA. Translation: AAO90900.1.
PIR	I40849.
RefSeq	NP_820386.1.
3D structure databases
HSSP	HSSP built from PDB template 1NEK based on UniProtKB P10444.
ModBase	Search...
Genome annotation databases
GeneID	1209307.
GenomeReviews	Gene locus CBU_1401 in contig AE016828_GR.
KEGG	cbu:CBU_1401.
TIGR	CBU_1401.
Phylogenomic databases
HOGENOM	P51054.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_1401-MON.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR003952. FRD_SDH_FAD_BS. IPR004112. Fum_Rdtase/Succ_DHase_flav_C. IPR011281. Succ_DHase_flav_su_fwd. IPR014006. Succ_Dhase_frdA_Gneg. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
TIGRFAMs	TIGR01816. sdhA_forward. 1 hit. TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITE	PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSA_COXBU

Accession

Primary (citable) accession number: P51054

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)