P51053 (DHSB_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinate dehydrogenase iron-sulfur subunit
EC=1.3.99.1

Gene names

Name:	sdhB
Ordered Locus Names:	CBU_1400

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster. Binds 1 3Fe-4S cluster. Binds 1 4Fe-4S cluster.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (bacterial route): step 1/1.
Subunit structure	Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur protein, cytochrome b-556 and a hydrophobic protein.
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 4Fe-4S ferredoxin-type domain.
Sequence caution	The sequence AAO90899.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA54873.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW succinate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	Succinate dehydrogenase iron-sulfur subunit		PRO_0000158687
Regions
	Domain	133 – 163	31	4Fe-4S ferredoxin-type
Sites
	Metal binding	53	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	58	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	73	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	143	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	146	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	149	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	153	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	200	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	206	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	210	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Binding site	158	1	Ubiquinone; shared with SdhD subunit By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51053 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 5949664A63BF5B41	FASTA	235	26,847
	10 20 30 40 50 60 MTFSIMRFNP ETDKKPYMQD FELDVSAIQG KMLLNALEAL REKHPDIGLR RSCAEGVCGS 70 80 90 100 110 120 DGMNINGKNA LACVTQLKDL PDRVVVRPLP GFPIIRDLIV DMEQFYAQYK KVKPYLLNDQ 130 140 150 160 170 180 EAPQKERLQS PEERAKLDGL YECILCACCS SSCPSYWWNP DKFIGPAGLL WSYRFIADSR 190 200 210 220 230 DSKEKERLDA MKDPYSVFRC RTIMDCATVC PKNLNPAKAI RKIRTEMLQE TESGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the succinate dehydrogenase-encoding gene cluster (sdh) from the rickettsia Coxiella burnetii." Heinzen R.A., Mo Y.-Y., Robertson S.J., Mallavia L.P. Gene 155:27-34(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Nine Mile.
[2]	Thiele D., Willems H., Oswald W., Krauss H. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Nine Mile.
[3]	"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L33409 Genomic DNA. Translation: AAA74134.1. X77919 Genomic DNA. Translation: CAA54873.1. Different initiation. AE016828 Genomic DNA. Translation: AAO90899.1. Different initiation.
PIR	S42873.
RefSeq	NP_820385.1. NC_002971.3.
3D structure databases
ProteinModelPortal	P51053.
SMR	P51053. Positions 1-229.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_1400.
Proteomic databases
PRIDE	P51053.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO90899; AAO90899; CBU_1400.
GeneID	1209306.
KEGG	cbu:CBU_1400.
PATRIC	17931557. VBICoxBur82552_1402.
Phylogenomic databases
eggNOG	COG0479.
HOGENOM	HOG000160590.
KO	K00240.
OMA	NCSRTCP.
ProtClustDB	PRK05950.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-1388-MONOMER.
UniPathway	UPA00223; UER01005.
Family and domain databases
Gene3D	1.10.1060.10. 1 hit. 3.10.20.30. 1 hit.
InterPro	IPR001041. 2Fe-2S_ferredoxin-type. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR012675. Beta-grasp_dom. IPR012285. Fum_reductase_C. IPR009051. Helical_ferredxn. IPR004489. Succ_DH/fum_Rdtase_Fe-S. IPR025192. Succ_DH/fum_Rdtase_N. [Graphical view]
Pfam	PF13085. Fer2_3. 1 hit. PF13183. Fer4_8. 1 hit. [Graphical view]
SUPFAM	SSF54292. Ferredoxin. 1 hit. SSF46548. Helical_ferredxn. 1 hit.
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSB_COXBU

Accession

Primary (citable) accession number: P51053

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program