P51040 (CISY_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Citrate synthase
EC=2.3.3.1

Gene names

Name:	gltA
Ordered Locus Names:	RF_1338

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.
Miscellaneous	Citrate synthase is found in nearly all cells capable of oxidative metabolism.
Sequence similarities	Belongs to the citrate synthase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	citrate (Si)-synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Citrate synthase		PRO_0000169962
Sites
	Active site	311	1	By similarity
	Active site	370	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51040 [UniParc]. Last modified July 19, 2005. Version 2. Checksum: 361CAB47B3274378	FASTA	435	49,100
	10 20 30 40 50 60 MTNENSNDSE FAELKIRGKI FKLPILKASI GEDVIDISRV SSEADCFTYD PGFMSTASCQ 70 80 90 100 110 120 STITYIDGDK GILRHRGYDI KDLAEKSDFL EVAYLLIYGE LPSIEQYNNF TKQVAHHSLV 130 140 150 160 170 180 NERLHYLFQT FCSSSHPMAI MLAAVGSLSA FYPDLLNFKE ADYELTAIRM IAKIPTIAAM 190 200 210 220 230 240 SYKYSIGQPF IYPDNSLDFT ENFLHMMFAT PCTKYKVNPI IKNALNKIFI LHADHEQNAS 250 260 270 280 290 300 TSTVRIAGSS GANPFACVST GIASLWGPAH GGANEAVINM LKEIGSSENI PKYIAKAKDK 310 320 330 340 350 360 NDPFRLMGFG HRVYKNYDPR AAVLKETCKE VLKELGQLEN NPLLQIAIEL EAIALKDEYF 370 380 390 400 410 420 IERKLYPNVD FYSGIIYKAM GIPSQMFTVL FAIARTVGWM AQWKEMHEDP EQKISRPRQL 430 YTGYVHREYK GIGER

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.
[2]	"Rickettsia felis: a new species of pathogenic rickettsia isolated from cat fleas." Higgins J.A., Radulovic S., Schriefer M.E., Azad A.F. J. Clin. Microbiol. 34:671-674(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 279-384.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY62189.1. U33922 Genomic DNA. Translation: AAB71724.1.
RefSeq	YP_247354.1. NC_007109.1.
3D structure databases
ProteinModelPortal	P51040.
SMR	P51040. Positions 12-430.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_1338.
Proteomic databases
PRIDE	P51040.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY62189; AAY62189; RF_1338.
GeneID	3400894.
KEGG	rfe:RF_1338.
PATRIC	17893602. VBIRicFel64634_1611.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0372.
HOGENOM	HOG000021224.
KO	K01647.
OMA	RVTHHTM.
ProtClustDB	PRK05614.
Enzyme and pathway databases
UniPathway	UPA00223; UER00717.
Family and domain databases
Gene3D	1.10.230.10. 1 hit. 1.10.580.10. 1 hit.
InterPro	IPR016142. Citrate_synth-like_lrg_a-sub. IPR016143. Citrate_synth-like_sm_a-sub. IPR002020. Citrate_synthase-like. IPR016141. Citrate_synthase-like_core. IPR019810. Citrate_synthase_AS. IPR024176. Citrate_synthase_bac-typ. IPR010953. Citrate_synthase_typ-II. [Graphical view]
PANTHER	PTHR11739. PTHR11739. 1 hit.
Pfam	PF00285. Citrate_synt. 1 hit. [Graphical view]
PIRSF	PIRSF001369. Citrate_synth. 1 hit.
PRINTS	PR00143. CITRTSNTHASE.
SUPFAM	SSF48256. Citrate_synthase_core. 1 hit.
TIGRFAMs	TIGR01798. cit_synth_I. 1 hit.
PROSITE	PS00480. CITRATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CISY_RICFE

Accession

Primary (citable) accession number: P51040
Secondary accession number(s): Q4UJV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 19, 2005
Last modified:	May 1, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program