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Protein

Citrate synthase, plasmid

Gene

pcsA

Organism
Rhizobium tropici
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The exact function of the plasmid-encoded citrate synthase is not clear, it could help nodulation by allowing the bacteria to use citrate as a chelator of iron and calcium.

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei306 – 3061PROSITE-ProRule annotation
Active sitei364 – 3641PROSITE-ProRule annotation

GO - Molecular functioni

  1. citrate (Si)-synthase activity Source: InterPro

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase, plasmid (EC:2.3.3.16)
Gene namesi
Name:pcsA
Encoded oniPlasmid sym0 Publication
OrganismiRhizobium tropici
Taxonomic identifieri398 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 429429Citrate synthase, plasmidPRO_0000169955Add
BLAST

Proteomic databases

PRIDEiP51038.

Structurei

3D structure databases

ProteinModelPortaliP51038.
SMRiP51038. Positions 4-429.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-I.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01798. cit_synth_I. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51038-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNNNACVLV DGHSAELKLR SSTIGPNVLG IGSLYEQTKM FTYDPGFTST
60 70 80 90 100
ASCESSITFI DGDEGVLLHR GYPIEQLAEH GDFLEVCYLL LYGELPTAAQ
110 120 130 140 150
KKDFDYRVVH HTMVHEQMSR FFTGFRRDAH PMAVMCGCVG ALSAFYHDST
160 170 180 190 200
DITDPHQRMV ASLRMIAKMP TLAAMAYKYH IGQPFVYPKN DLDYASNFLR
210 220 230 240 250
MCFAVPCEEY VVNPVLARAM DRIFILHADH EQNASTSTVR LAGSSGANPF
260 270 280 290 300
ACIAAGIACL WGPAHGGANE RALNMLTEIG TVDRIPEYIA RAKDKNDPFR
310 320 330 340 350
LMGFGHRVYK NYDPRAKIMQ KTAHEVLGEL GIKDDPLLDI AIELERIALT
360 370 380 390 400
DDYFIEKKLY PNVDFYSGIT LKALGFPTTM FTVLFALART VGWIAQWNEM
410 420
IEDPDQRIGR PRQLYTGAPL REYVPLSKR
Length:429
Mass (Da):48,105
Last modified:October 1, 1996 - v1
Checksum:iC2E4560E4FE03485
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34516 Genomic DNA. Translation: CAA84274.1.
PIRiS41527.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34516 Genomic DNA. Translation: CAA84274.1.
PIRiS41527.

3D structure databases

ProteinModelPortaliP51038.
SMRiP51038. Positions 4-429.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP51038.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-I.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01798. cit_synth_I. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nodulating ability of Rhizobium tropici is conditioned by a plasmid-encoded citrate synthase."
    Pardo M.A., Lagunez J., Miranda J., Martinez E.
    Mol. Microbiol. 11:315-321(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CFN 299.

Entry informationi

Entry nameiCISY2_RHITR
AccessioniPrimary (citable) accession number: P51038
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.