P51028 (WNT8A_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-8a

Gene names

Name:	wnt8a
Synonyms:	wnt-8, wnt8

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Is likely to signal over only few cell diameters. May be involved in the specification of the spatial patterns of expression of Gsc and other regulatory genes leading to the establishment of the embryonic axis.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	In future mesoderm.
Developmental stage	Required for mesoderm and neural ectoderm patterning during gastrulation. Ref.2
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway involved in dorsal/ventral axis specification Inferred from mutant phenotype. Source: BHF-UCL brain segmentation Inferred from mutant phenotype. Source: ZFIN canonical Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome cell fate commitment Inferred from direct assay. Source: ZFIN ectoderm development Inferred from mutant phenotype Ref.2. Source: ZFIN endoderm development Inferred from mutant phenotype. Source: ZFIN eye development Inferred from direct assay. Source: ZFIN hindbrain development Inferred from mutant phenotype. Source: ZFIN mesoderm formation Inferred from mutant phenotype. Source: ZFIN negative regulation of Wnt receptor signaling pathway Inferred from genetic interaction. Source: ZFIN neural plate pattern specification Inferred from direct assay. Source: ZFIN notochord development Inferred from mutant phenotype. Source: ZFIN polarity specification of anterior/posterior axis Inferred from Biological aspect of Ancestor. Source: RefGenome polarity specification of proximal/distal axis Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of fibroblast growth factor receptor signaling pathway Inferred from mutant phenotype. Source: ZFIN somitogenesis Inferred from genetic interaction. Source: ZFIN spinal cord anterior/posterior patterning Inferred from mutant phenotype. Source: ZFIN tail morphogenesis Inferred from mutant phenotype. Source: ZFIN
Cellular component	extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome intracellular Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	G-protein coupled receptor binding Inferred from Biological aspect of Ancestor. Source: RefGenome zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 359	334	Protein Wnt-8a		PRO_0000041446
Amino acid modifications
	Glycosylation	104	1	N-linked (GlcNAc...) Potential
	Glycosylation	263	1	N-linked (GlcNAc...) Potential
	Glycosylation	282	1	N-linked (GlcNAc...) Potential
	Glycosylation	348	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	244	1	A → S in AAH55535. Ref.3
	Sequence conflict	354	1	Q → R in AAH55535. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P51028 [UniParc]. Last modified May 27, 2002. Version 2. Checksum: C192475B9D48C3C2	FASTA	359	40,289
	10 20 30 40 50 60 MNPCQIFASL VMSICCHILS STAWSVNNFL MTGPKAYLAY TSSVQAGAQS GIEECKHQFA 70 80 90 100 110 120 WDRWNCPESA LQLSTHKGLR SATRETAFVH AISAAGVMYT LTKNCSMGDF ENCGCDDSKI 130 140 150 160 170 180 GKMGGRGWVW GGCSDNVNFG DRIAKLFVDA LENGHDSRAA VNLHNNEAGR LAVKATLKRT 190 200 210 220 230 240 CKCHGLSGSC SIQTCWMQLA DFRDIGSYLK IKHDQARKLE MDKIRMRAGN SADNRGAIAD 250 260 270 280 290 300 TFSAVARTEL IFMEDSPDYC VKNLSMGLHG TEGRECLQSG KNLSQWERRS CRRLCHECGL 310 320 330 340 350 KVEERRIETV SSCNCKFHWC CTVKCETCTQ TVTRYFCAKR HRNRRPHNHS RKRQHTRRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Zebrafish wnt8 and wnt8b share a common activity but are involved in distinct developmental pathways." Kelly G.M., Erezyilmaz D.F., Greenstein P.E., Moon R.T. Development 121:1787-1799(1995) [PubMed: 7600994] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[2]	"Zebrafish wnt8 encodes two wnt8 proteins on a bicistronic transcript and is required for mesoderm and neurectoderm patterning." Lekven A.C., Thorpe C.J., Waxman J.S., Moon R.T. Dev. Cell 1:103-114(2001) [PubMed: 11703928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION, DEVELOPMENTAL STAGE.
[3]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: AB.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10869 mRNA. Translation: AAC59697.2. AY032749 Genomic DNA. Translation: AAK70223.1. BC055535 mRNA. Translation: AAH55535.1.
IPI	IPI00498714.
PIR	I50505.
RefSeq	NP_571021.2. NM_130946.2.
UniGene	Dr.309.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P51028.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000017635; ENSDARP00000011496; ENSDARG00000052910.
GeneID	30122.
KEGG	dre:30122.
Organism-specific databases
CTD	7478.
ZFIN	ZDB-GENE-980526-332. wnt8a.
Phylogenomic databases
eggNOG	fiNOG07549.
GeneTree	ENSGT00600000084119.
HOVERGEN	HBG001595.
InParanoid	P51028.
Gene expression databases
ArrayExpress	P51028.
Bgee	P51028.
Family and domain databases
InterPro	IPR005817. Wnt. IPR013301. Wnt8. IPR018161. Wnt_grthfactor_CS. IPR007087. Znf_C2H2. [Graphical view]
KO	K00714.
PANTHER	PTHR12027. Wnt. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01892. WNT8PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WNT8A_DANRE

Accession

Primary (citable) accession number: P51028
Secondary accession number(s): Q7SXM4, Q90YL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 27, 2002
Last modified:	January 25, 2012
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program