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Protein

S-formylglutathione hydrolase FrmB

Gene

frmB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Serine hydrolase involved in the detoxification of formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate. Shows also esterase activity against two pNP-esters (pNP-acetate and pNP-propionate), alpha-naphthyl acetate and lactoylglutathione.1 Publication

Catalytic activityi

S-formylglutathione + H2O = glutathione + formate.1 Publication

Enzyme regulationi

Inhibited by the sulfhydryl inhibitors (N-ethylmaleimide, iodoacetate, ZnCl2 and CuCl2).1 Publication

Kineticsi

  1. KM=0.41 mM for S-formylglutathione1 Publication
  2. KM=0.29 mM for pNP-acetate1 Publication
  3. KM=0.83 mM for pNP-propionate1 Publication
  4. KM=0.60 mM for S-lactoylglutathione1 Publication
  1. Vmax=55.0 µmol/min/mg enzyme with S-formylglutathione as substrate1 Publication
  2. Vmax=0.58 µmol/min/mg enzyme with pNP-acetate as substrate1 Publication
  3. Vmax=0.27 µmol/min/mg enzyme with pNP-propionate as substrate1 Publication
  4. Vmax=0.09 µmol/min/mg enzyme with S-lactoylglutathione as substrate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei145 – 1451Charge relay systemBy similarity
Active sitei221 – 2211Charge relay systemBy similarity
Active sitei254 – 2541Charge relay systemBy similarity

GO - Molecular functioni

  • carboxylic ester hydrolase activity Source: UniProtKB-KW
  • S-formylglutathione hydrolase activity Source: EcoCyc

GO - Biological processi

  • formaldehyde catabolic process Source: InterPro
  • formaldehyde metabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

BioCyciEcoCyc:G6208-MONOMER.
ECOL316407:JW0346-MONOMER.
MetaCyc:G6208-MONOMER.
SABIO-RKP51025.

Protein family/group databases

ESTHERiecoli-yaim. A85-EsteraseD-FGH.
MEROPSiS09.A38.

Names & Taxonomyi

Protein namesi
Recommended name:
S-formylglutathione hydrolase FrmB (EC:3.1.2.12)
Short name:
FGH
Gene namesi
Name:frmB
Synonyms:yaiM
Ordered Locus Names:b0355, JW0346
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13295. frmB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 277277S-formylglutathione hydrolase FrmBPRO_0000210343Add
BLAST

Proteomic databases

PaxDbiP51025.

Expressioni

Inductioni

Induced by formaldehyde and repressed by FrmR.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4261809. 10 interactions.
DIPiDIP-11274N.
IntActiP51025. 5 interactions.
STRINGi511145.b0355.

Structurei

3D structure databases

ProteinModelPortaliP51025.
SMRiP51025. Positions 1-271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the esterase D family.Curated

Phylogenomic databases

eggNOGiENOG4105C4W. Bacteria.
COG0627. LUCA.
HOGENOMiHOG000263929.
InParanoidiP51025.
KOiK01070.
OMAiKWHAERL.
PhylomeDBiP51025.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000801. Esterase_put.
IPR014186. S-formylglutathione_hydrol.
[Graphical view]
PANTHERiPTHR10061. PTHR10061. 1 hit.
PfamiPF00756. Esterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR02821. fghA_ester_D. 1 hit.

Sequencei

Sequence statusi: Complete.

P51025-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELIEKHVSF GGWQNMYRHY SQSLKCEMNV GVYLPPKAAN EKLPVLYWLS
60 70 80 90 100
GLTCNEQNFI TKSGMQRYAA EHNIIVVAPD TSPRGSHVAD ADRYDLGQGA
110 120 130 140 150
GFYLNATQAP WNEHYKMYDY IRNELPDLVM HHFPATAKKS ISGHSMGGLG
160 170 180 190 200
ALVLALRNPD EYVSVSAFSP IVSPSQVPWG QQAFAAYLAE NKDAWLDYDP
210 220 230 240 250
VSLISQGQRV AEIMVDQGLS DDFYAEQLRT PNLEKICQEM NIKTLIRYHE
260 270
GYDHSYYFVS SFIGEHIAYH ANKLNMR
Length:277
Mass (Da):31,424
Last modified:November 1, 1997 - v2
Checksum:iC69C708293FA0AEC
GO

Sequence cautioni

The sequence D85613 differs from that shown. Reason: Frameshift at position 137. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85613 Genomic DNA. No translation available.
U73857 Genomic DNA. Translation: AAB18080.1.
U00096 Genomic DNA. Translation: AAC73458.1.
AP009048 Genomic DNA. Translation: BAE76137.1.
PIRiC64763.
RefSeqiNP_414889.1. NC_000913.3.
WP_000419081.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73458; AAC73458; b0355.
BAE76137; BAE76137; BAE76137.
GeneIDi944991.
KEGGiecj:JW0346.
eco:b0355.
PATRICi32115849. VBIEscCol129921_0366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85613 Genomic DNA. No translation available.
U73857 Genomic DNA. Translation: AAB18080.1.
U00096 Genomic DNA. Translation: AAC73458.1.
AP009048 Genomic DNA. Translation: BAE76137.1.
PIRiC64763.
RefSeqiNP_414889.1. NC_000913.3.
WP_000419081.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP51025.
SMRiP51025. Positions 1-271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261809. 10 interactions.
DIPiDIP-11274N.
IntActiP51025. 5 interactions.
STRINGi511145.b0355.

Protein family/group databases

ESTHERiecoli-yaim. A85-EsteraseD-FGH.
MEROPSiS09.A38.

Proteomic databases

PaxDbiP51025.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73458; AAC73458; b0355.
BAE76137; BAE76137; BAE76137.
GeneIDi944991.
KEGGiecj:JW0346.
eco:b0355.
PATRICi32115849. VBIEscCol129921_0366.

Organism-specific databases

EchoBASEiEB3080.
EcoGeneiEG13295. frmB.

Phylogenomic databases

eggNOGiENOG4105C4W. Bacteria.
COG0627. LUCA.
HOGENOMiHOG000263929.
InParanoidiP51025.
KOiK01070.
OMAiKWHAERL.
PhylomeDBiP51025.

Enzyme and pathway databases

BioCyciEcoCyc:G6208-MONOMER.
ECOL316407:JW0346-MONOMER.
MetaCyc:G6208-MONOMER.
SABIO-RKP51025.

Miscellaneous databases

PROiP51025.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000801. Esterase_put.
IPR014186. S-formylglutathione_hydrol.
[Graphical view]
PANTHERiPTHR10061. PTHR10061. 1 hit.
PfamiPF00756. Esterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR02821. fghA_ester_D. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSFGH1_ECOLI
AccessioniPrimary (citable) accession number: P51025
Secondary accession number(s): P77317, Q2MC69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.