Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

S-formylglutathione hydrolase FrmB

Gene

frmB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine hydrolase involved in the detoxification of formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate. Shows also esterase activity against two pNP-esters (pNP-acetate and pNP-propionate), alpha-naphthyl acetate and lactoylglutathione.1 Publication

Catalytic activityi

S-formylglutathione + H2O = glutathione + formate.1 Publication

Enzyme regulationi

Inhibited by the sulfhydryl inhibitors (N-ethylmaleimide, iodoacetate, ZnCl2 and CuCl2).1 Publication

Kineticsi

  1. KM=0.41 mM for S-formylglutathione1 Publication
  2. KM=0.29 mM for pNP-acetate1 Publication
  3. KM=0.83 mM for pNP-propionate1 Publication
  4. KM=0.60 mM for S-lactoylglutathione1 Publication
  1. Vmax=55.0 µmol/min/mg enzyme with S-formylglutathione as substrate1 Publication
  2. Vmax=0.58 µmol/min/mg enzyme with pNP-acetate as substrate1 Publication
  3. Vmax=0.27 µmol/min/mg enzyme with pNP-propionate as substrate1 Publication
  4. Vmax=0.09 µmol/min/mg enzyme with S-lactoylglutathione as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei145Charge relay systemBy similarity1
Active sitei221Charge relay systemBy similarity1
Active sitei254Charge relay systemBy similarity1

GO - Molecular functioni

  • carboxylic ester hydrolase activity Source: UniProtKB-KW
  • S-formylglutathione hydrolase activity Source: EcoCyc

GO - Biological processi

  • formaldehyde catabolic process Source: InterPro
  • formaldehyde metabolic process Source: EcoCyc

Keywordsi

Molecular functionHydrolase, Serine esterase

Enzyme and pathway databases

BioCyciEcoCyc:G6208-MONOMER
MetaCyc:G6208-MONOMER
SABIO-RKiP51025

Protein family/group databases

ESTHERiecoli-yaim A85-EsteraseD-FGH

Names & Taxonomyi

Protein namesi
Recommended name:
S-formylglutathione hydrolase FrmB (EC:3.1.2.12)
Short name:
FGH
Gene namesi
Name:frmB
Synonyms:yaiM
Ordered Locus Names:b0355, JW0346
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13295 frmB

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002103431 – 277S-formylglutathione hydrolase FrmBAdd BLAST277

Proteomic databases

PaxDbiP51025
PRIDEiP51025

Expressioni

Inductioni

Induced by formaldehyde and repressed by FrmR.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4261809, 19 interactors
DIPiDIP-11274N
IntActiP51025, 5 interactors
STRINGi316385.ECDH10B_0310

Structurei

3D structure databases

ProteinModelPortaliP51025
SMRiP51025
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the esterase D family.Curated

Phylogenomic databases

eggNOGiENOG4105C4W Bacteria
COG0627 LUCA
HOGENOMiHOG000263929
InParanoidiP51025
KOiK01070
OMAiWAPHYRM
PhylomeDBiP51025

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000801 Esterase_put
IPR014186 S-formylglutathione_hydrol
PANTHERiPTHR10061 PTHR10061, 1 hit
PfamiView protein in Pfam
PF00756 Esterase, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
TIGRFAMsiTIGR02821 fghA_ester_D, 1 hit

Sequencei

Sequence statusi: Complete.

P51025-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELIEKHVSF GGWQNMYRHY SQSLKCEMNV GVYLPPKAAN EKLPVLYWLS
60 70 80 90 100
GLTCNEQNFI TKSGMQRYAA EHNIIVVAPD TSPRGSHVAD ADRYDLGQGA
110 120 130 140 150
GFYLNATQAP WNEHYKMYDY IRNELPDLVM HHFPATAKKS ISGHSMGGLG
160 170 180 190 200
ALVLALRNPD EYVSVSAFSP IVSPSQVPWG QQAFAAYLAE NKDAWLDYDP
210 220 230 240 250
VSLISQGQRV AEIMVDQGLS DDFYAEQLRT PNLEKICQEM NIKTLIRYHE
260 270
GYDHSYYFVS SFIGEHIAYH ANKLNMR
Length:277
Mass (Da):31,424
Last modified:November 1, 1997 - v2
Checksum:iC69C708293FA0AEC
GO

Sequence cautioni

The sequence D85613 differs from that shown. Reason: Frameshift at position 137.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85613 Genomic DNA No translation available.
U73857 Genomic DNA Translation: AAB18080.1
U00096 Genomic DNA Translation: AAC73458.1
AP009048 Genomic DNA Translation: BAE76137.1
PIRiC64763
RefSeqiNP_414889.1, NC_000913.3
WP_000419081.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73458; AAC73458; b0355
BAE76137; BAE76137; BAE76137
GeneIDi944991
KEGGiecj:JW0346
eco:b0355
PATRICifig|1411691.4.peg.1923

Similar proteinsi

Entry informationi

Entry nameiSFGH1_ECOLI
AccessioniPrimary (citable) accession number: P51025
Secondary accession number(s): P77317, Q2MC69
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health