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Protein

ETS-like protein pointed, isoform P2/D

Gene

pnt

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for glial-neuronal cell interactions at the ventral midline which are necessary for the proper elaboration of commissures in the embryonic CNS.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi610 – 69081ETSPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • repressing transcription factor binding Source: FlyBase
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: FlyBase
  • sequence-specific DNA binding Source: InterPro

GO - Biological processi

  • determination of adult lifespan Source: FlyBase
  • determination of genital disc primordium Source: FlyBase
  • epidermal growth factor receptor signaling pathway Source: FlyBase
  • epithelial cell migration, open tracheal system Source: FlyBase
  • epithelial cell proliferation involved in Malpighian tubule morphogenesis Source: FlyBase
  • eye-antennal disc morphogenesis Source: FlyBase
  • heart development Source: FlyBase
  • heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: FlyBase
  • imaginal disc-derived wing morphogenesis Source: FlyBase
  • muscle fiber development Source: FlyBase
  • negative regulation of apoptotic signaling pathway Source: FlyBase
  • negative regulation of cardioblast cell fate specification Source: FlyBase
  • open tracheal system development Source: FlyBase
  • ovarian follicle cell development Source: FlyBase
  • pericardial nephrocyte differentiation Source: FlyBase
  • peripheral nervous system development Source: FlyBase
  • phagocytosis Source: FlyBase
  • positive regulation of cell proliferation Source: FlyBase
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • primary branching, open tracheal system Source: FlyBase
  • R3/R4 cell fate commitment Source: FlyBase
  • R7 cell development Source: FlyBase
  • Ras protein signal transduction Source: FlyBase
  • regulation of hemocyte differentiation Source: FlyBase
  • regulation of neurogenesis Source: FlyBase
  • secondary branching, open tracheal system Source: FlyBase
  • terminal region determination Source: FlyBase
  • torso signaling pathway Source: FlyBase
  • trachea development Source: FlyBase
  • tracheal outgrowth, open tracheal system Source: FlyBase
  • trachea morphogenesis Source: FlyBase
  • transcription from RNA polymerase II promoter Source: GOC
  • wing disc dorsal/ventral pattern formation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-DME-2559585. Oncogene Induced Senescence.
SignaLinkiP51023.

Names & Taxonomyi

Protein namesi
Recommended name:
ETS-like protein pointed, isoform P2/D
Short name:
D-ETS-2
Gene namesi
Name:pnt
Synonyms:ETS2, Ets58AB
ORF Names:CG17077
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0003118. pnt.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 718718ETS-like protein pointed, isoform P2/DPRO_0000204130Add
BLAST

Proteomic databases

PaxDbiP51023.

Expressioni

Tissue specificityi

Expressed in a complex dynamic pattern in early embryos, including the midline and midline glial cells.2 Publications

Developmental stagei

Expressed throughout development with lower levels during larval development.1 Publication

Gene expression databases

BgeeiP51023.
ExpressionAtlasiP51023. differential.
GenevisibleiP51023. DM.

Interactioni

GO - Molecular functioni

  • repressing transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi67700. 26 interactions.
DIPiDIP-19576N.
IntActiP51023. 4 interactions.
MINTiMINT-2737387.
STRINGi7227.FBpp0088658.

Structurei

3D structure databases

ProteinModelPortaliP51023.
SMRiP51023. Positions 147-249, 604-690.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini164 – 25087PNTPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi341 – 3477Poly-Gln
Compositional biasi359 – 3624Poly-Asn
Compositional biasi389 – 3935Poly-Asn
Compositional biasi405 – 41511Poly-AsnAdd
BLAST
Compositional biasi418 – 4214Poly-Ala
Compositional biasi481 – 4844Poly-Gly

Sequence similaritiesi

Belongs to the ETS family.Curated
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation
Contains 1 PNT (pointed) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3806. Eukaryota.
ENOG410Z0ZF. LUCA.
GeneTreeiENSGT00760000118907.
InParanoidiP51023.
KOiK02678.
OMAiCALGKEA.
PhylomeDBiP51023.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform P2 (identifier: P51023-1) [UniParc]FASTAAdd to basket

Also known as: B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELAICKTDL SATKFMLPPA LPSSAAIGSS SAVASTASHF LDKAAHELFQ
60 70 80 90 100
LNAINGHLFK SPASSHLNSV GSPSILSQLN GIGNSGNHSG QVSTMRLKKN
110 120 130 140 150
RKVTFLSSLV ESKNIFIKEE PIHGCKDLCS LSDISDHEAS LEVPTALPPL
160 170 180 190 200
TPGTNRKVNE VLKASFASWE KEVQKCNITK DPREWTEEHV IYWLNWAKNE
210 220 230 240 250
FSLVSMNLDP FYKMKGRAMV DLGKEKFLAI TPPFTGDILW EHLDILQKDC
260 270 280 290 300
EKPNEDIVHG NSFESATTAS VCGSDHQVAN YPNETHANSN NSSINSRLSM
310 320 330 340 350
DYVTSAGNSD NKNFHRATPH SHNGYNTSNT HDRINNSTPP QQQQSQQPTV
360 370 380 390 400
NGSGSASSNN NNSMLPPAVQ QSNNENNNTS SSNTNNSSNN NNNSGGSNNS
410 420 430 440 450
NAGSNNNNNN NNNINFMAAA AIFQHHLKEE PGTQNGNIGG YGGGSNSQND
460 470 480 490 500
PTDLSSYGLP AHLAAYGGGS GSGPTGGRSS GGGGDESDYH STISAQDHQS
510 520 530 540 550
QQSSGGNGSG GASGGSTGNS NGYLDSSSEF YGSYAGRNRF HDGYPPEFTP
560 570 580 590 600
YDAQSFQSMG PQPTAMDQWG AAHAHQHPAA YMSTLGLDKG LLGGYTTQGG
610 620 630 640 650
VPCFTGSGPI QLWQFLLELL LDKTCQSFIS WTGDGWEFKL TDPDEVARRW
660 670 680 690 700
GIRKNKPKMN YEKLSRGLRY YYDKNIIHKT AGKRYVYRFV CDLQNLVGHT
710
PEELVAKYDL KIEKKDVD
Length:718
Mass (Da):77,683
Last modified:November 16, 2001 - v2
Checksum:iFD6AFD0F4BCD69C5
GO
Isoform D (identifier: P51023-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-141: ELAICKTDLS...SDISDHEASL → TNEWIDWNDS...QLTTNHSKLK

Note: No experimental confirmation available.
Show »
Length:636
Mass (Da):69,890
Checksum:iF868CB584F432C9B
GO
Isoform P1 (identifier: P51022-1) [UniParc]FASTAAdd to basket

Also known as: C

The sequence of this isoform can be found in the external entry P51022.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:623
Mass (Da):66,866
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti133 – 1353DIS → VYP in CAA48917 (PubMed:8223245).Curated
Sequence conflicti538 – 5381N → G in ABC86249 (Ref. 4) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei2 – 141140ELAIC…HEASL → TNEWIDWNDSRMLPPLRSAN YNYHPQTFLPNNYQCFTGKF HLKGQKLQQLTTNHSKLK in isoform D. 1 PublicationVSP_037668Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69167 mRNA. Translation: CAA48917.1.
AE014297 Genomic DNA. Translation: AAF56125.1.
AE014297 Genomic DNA. Translation: AAN13942.1.
BT024187 mRNA. Translation: ABC86249.1.
BT058033 mRNA. Translation: ACM16754.1.
BT001893 mRNA. Translation: AAN71682.1. Sequence problems.
M88472 mRNA. Translation: AAC34200.1.
PIRiS33168.
RefSeqiNP_524461.2. NM_079737.3. [P51023-1]
NP_732857.1. NM_170651.4. [P51023-2]
UniGeneiDm.2370.

Genome annotation databases

EnsemblMetazoaiFBtr0089717; FBpp0088658; FBgn0003118. [P51023-1]
GeneIDi42757.
KEGGidme:Dmel_CG17077.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69167 mRNA. Translation: CAA48917.1.
AE014297 Genomic DNA. Translation: AAF56125.1.
AE014297 Genomic DNA. Translation: AAN13942.1.
BT024187 mRNA. Translation: ABC86249.1.
BT058033 mRNA. Translation: ACM16754.1.
BT001893 mRNA. Translation: AAN71682.1. Sequence problems.
M88472 mRNA. Translation: AAC34200.1.
PIRiS33168.
RefSeqiNP_524461.2. NM_079737.3. [P51023-1]
NP_732857.1. NM_170651.4. [P51023-2]
UniGeneiDm.2370.

3D structure databases

ProteinModelPortaliP51023.
SMRiP51023. Positions 147-249, 604-690.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi67700. 26 interactions.
DIPiDIP-19576N.
IntActiP51023. 4 interactions.
MINTiMINT-2737387.
STRINGi7227.FBpp0088658.

Proteomic databases

PaxDbiP51023.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0089717; FBpp0088658; FBgn0003118. [P51023-1]
GeneIDi42757.
KEGGidme:Dmel_CG17077.

Organism-specific databases

CTDi42757.
FlyBaseiFBgn0003118. pnt.

Phylogenomic databases

eggNOGiKOG3806. Eukaryota.
ENOG410Z0ZF. LUCA.
GeneTreeiENSGT00760000118907.
InParanoidiP51023.
KOiK02678.
OMAiCALGKEA.
PhylomeDBiP51023.

Enzyme and pathway databases

ReactomeiR-DME-2559585. Oncogene Induced Senescence.
SignaLinkiP51023.

Miscellaneous databases

ChiTaRSipnt. fly.
GenomeRNAii42757.

Gene expression databases

BgeeiP51023.
ExpressionAtlasiP51023. differential.
GenevisibleiP51023. DM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Drosophila gene pointed encodes two ETS-like proteins which are involved in the development of the midline glial cells."
    Klaembt C.
    Development 117:163-176(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM P2), ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Stapleton M., Carlson J.W., Booth B., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS D AND P2).
    Strain: Berkeley.
    Tissue: Embryo.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-323 (ISOFORM P2).
    Strain: Berkeley.
    Tissue: Embryo.
  6. "Isolation and characterization of five Drosophila genes that encode an ets-related DNA binding domain."
    Chen T., Bunting M., Karim F.D., Thummel C.S.
    Dev. Biol. 151:176-191(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 551-708 (ISOFORMS D/P2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Canton-S.
    Tissue: Larva.

Entry informationi

Entry nameiPNT2_DROME
AccessioniPrimary (citable) accession number: P51023
Secondary accession number(s): B9EQV8
, Q29R53, Q8IG92, Q8IMZ1, Q9VCN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 16, 2001
Last modified: July 6, 2016
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.