P51023 (PNT2_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ETS-like protein pointed, isoform P2/D
Short name=D-ETS-2

Gene names

Name:	pnt
Synonyms:	ETS2, Ets58AB
ORF Names:	CG17077

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	718 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required for glial-neuronal cell interactions at the ventral midline which are necessary for the proper elaboration of commissures in the embryonic CNS.
Subcellular location	Nucleus Potential.
Tissue specificity	Expressed in a complex dynamic pattern in early embryos, including the midline and midline glial cells. Ref.1 Ref.6
Developmental stage	Expressed throughout development with lower levels during larval development. Ref.6
Sequence similarities	Belongs to the ETS family. Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Uncategorized	? Inferred from mutant phenotype. Source: FlyBase
Biological process	R3/R4 cell fate commitment Inferred from mutant phenotype. Source: FlyBase R7 cell development Traceable author statement. Source: FlyBase Ras protein signal transduction Traceable author statement. Source: FlyBase anti-apoptosis Inferred from genetic interaction. Source: FlyBase determination of genital disc primordium Inferred from mutant phenotype. Source: FlyBase epidermal growth factor receptor signaling pathway Non-traceable author statement. Source: FlyBase epithelial cell migration, open tracheal system Inferred from mutant phenotype. Source: FlyBase eye-antennal disc morphogenesis Inferred from mutant phenotype. Source: FlyBase imaginal disc-derived wing morphogenesis Inferred from mutant phenotype. Source: FlyBase muscle fiber development Inferred from mutant phenotype. Source: FlyBase negative regulation of cardioblast cell fate specification Inferred from mutant phenotype. Source: FlyBase ovarian follicle cell development Traceable author statement. Source: FlyBase peripheral nervous system development Traceable author statement. Source: FlyBase phagocytosis, engulfment Inferred from mutant phenotype. Source: FlyBase positive regulation of cell proliferation Inferred from mutant phenotype. Source: FlyBase primary branching, open tracheal system Inferred from mutant phenotype. Source: FlyBase regulation of hemocyte differentiation Inferred from mutant phenotype. Source: FlyBase secondary branching, open tracheal system Inferred from mutant phenotype. Source: FlyBase terminal region determination Inferred from genetic interaction. Source: FlyBase torso signaling pathway Inferred from genetic interaction. Source: FlyBase tracheal outgrowth, open tracheal system Inferred from mutant phenotype. Source: FlyBase wing disc dorsal/ventral pattern formation Inferred from genetic interaction. Source: FlyBase
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: FlyBase sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform P2 (identifier: P51023-1)

Also known as: B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform D (identifier: P51023-2)

The sequence of this isoform differs from the canonical sequence as follows:
     2-141: ELAICKTDLS...SDISDHEASL → TNEWIDWNDS...QLTTNHSKLK

Note: No experimental confirmation available.

Isoform P1 (identifier: P51022-1)

Also known as: C;

The sequence of this isoform can be found in the external entry P51022.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 718	718	ETS-like protein pointed, isoform P2/D		PRO_0000204130
Regions
	Domain	164 – 250	87	PNT
	DNA binding	610 – 690	81	ETS
	Compositional bias	341 – 347	7	Poly-Gln
	Compositional bias	359 – 362	4	Poly-Asn
	Compositional bias	389 – 393	5	Poly-Asn
	Compositional bias	405 – 415	11	Poly-Asn
	Compositional bias	418 – 421	4	Poly-Ala
	Compositional bias	481 – 484	4	Poly-Gly
Natural variations
	Alternative sequence	2 – 141	140	ELAIC…HEASL → TNEWIDWNDSRMLPPLRSAN YNYHPQTFLPNNYQCFTGKF HLKGQKLQQLTTNHSKLK in isoform D.		VSP_037668
Experimental info
	Sequence conflict	133 – 135	3	DIS → VYP in CAA48917. Ref.1
	Sequence conflict	538	1	N → G in ABC86249. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform P2 (B) [UniParc]. Last modified November 16, 2001. Version 2. Checksum: FD6AFD0F4BCD69C5	FASTA	718	77,683
	10 20 30 40 50 60 MELAICKTDL SATKFMLPPA LPSSAAIGSS SAVASTASHF LDKAAHELFQ LNAINGHLFK 70 80 90 100 110 120 SPASSHLNSV GSPSILSQLN GIGNSGNHSG QVSTMRLKKN RKVTFLSSLV ESKNIFIKEE 130 140 150 160 170 180 PIHGCKDLCS LSDISDHEAS LEVPTALPPL TPGTNRKVNE VLKASFASWE KEVQKCNITK 190 200 210 220 230 240 DPREWTEEHV IYWLNWAKNE FSLVSMNLDP FYKMKGRAMV DLGKEKFLAI TPPFTGDILW 250 260 270 280 290 300 EHLDILQKDC EKPNEDIVHG NSFESATTAS VCGSDHQVAN YPNETHANSN NSSINSRLSM 310 320 330 340 350 360 DYVTSAGNSD NKNFHRATPH SHNGYNTSNT HDRINNSTPP QQQQSQQPTV NGSGSASSNN 370 380 390 400 410 420 NNSMLPPAVQ QSNNENNNTS SSNTNNSSNN NNNSGGSNNS NAGSNNNNNN NNNINFMAAA 430 440 450 460 470 480 AIFQHHLKEE PGTQNGNIGG YGGGSNSQND PTDLSSYGLP AHLAAYGGGS GSGPTGGRSS 490 500 510 520 530 540 GGGGDESDYH STISAQDHQS QQSSGGNGSG GASGGSTGNS NGYLDSSSEF YGSYAGRNRF 550 560 570 580 590 600 HDGYPPEFTP YDAQSFQSMG PQPTAMDQWG AAHAHQHPAA YMSTLGLDKG LLGGYTTQGG 610 620 630 640 650 660 VPCFTGSGPI QLWQFLLELL LDKTCQSFIS WTGDGWEFKL TDPDEVARRW GIRKNKPKMN 670 680 690 700 710 YEKLSRGLRY YYDKNIIHKT AGKRYVYRFV CDLQNLVGHT PEELVAKYDL KIEKKDVD
	Isoform D [UniParc]. Checksum: F868CB584F432C9B	FASTA	636	69,890
	10 20 30 40 50 60 MTNEWIDWND SRMLPPLRSA NYNYHPQTFL PNNYQCFTGK FHLKGQKLQQ LTTNHSKLKE 70 80 90 100 110 120 VPTALPPLTP GTNRKVNEVL KASFASWEKE VQKCNITKDP REWTEEHVIY WLNWAKNEFS 130 140 150 160 170 180 LVSMNLDPFY KMKGRAMVDL GKEKFLAITP PFTGDILWEH LDILQKDCEK PNEDIVHGNS 190 200 210 220 230 240 FESATTASVC GSDHQVANYP NETHANSNNS SINSRLSMDY VTSAGNSDNK NFHRATPHSH 250 260 270 280 290 300 NGYNTSNTHD RINNSTPPQQ QQSQQPTVNG SGSASSNNNN SMLPPAVQQS NNENNNTSSS 310 320 330 340 350 360 NTNNSSNNNN NSGGSNNSNA GSNNNNNNNN NINFMAAAAI FQHHLKEEPG TQNGNIGGYG 370 380 390 400 410 420 GGSNSQNDPT DLSSYGLPAH LAAYGGGSGS GPTGGRSSGG GGDESDYHST ISAQDHQSQQ 430 440 450 460 470 480 SSGGNGSGGA SGGSTGNSNG YLDSSSEFYG SYAGRNRFHD GYPPEFTPYD AQSFQSMGPQ 490 500 510 520 530 540 PTAMDQWGAA HAHQHPAAYM STLGLDKGLL GGYTTQGGVP CFTGSGPIQL WQFLLELLLD 550 560 570 580 590 600 KTCQSFISWT GDGWEFKLTD PDEVARRWGI RKNKPKMNYE KLSRGLRYYY DKNIIHKTAG 610 620 630 KRYVYRFVCD LQNLVGHTPE ELVAKYDLKI EKKDVD
	Isoform P1 (C) [UniParc]. See P51022.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila gene pointed encodes two ETS-like proteins which are involved in the development of the midline glial cells." Klaembt C. Development 117:163-176(1993) [PubMed: 8223245] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM P2), ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[4]	Stapleton M., Carlson J.W., Booth B., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS D AND P2). Strain: Berkeley. Tissue: Embryo.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-323 (ISOFORM P2). Strain: Berkeley. Tissue: Embryo.
[6]	"Isolation and characterization of five Drosophila genes that encode an ets-related DNA binding domain." Chen T., Bunting M., Karim F.D., Thummel C.S. Dev. Biol. 151:176-191(1992) [PubMed: 1577186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 551-708 (ISOFORMS D/P2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: Canton-S. Tissue: Larva.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69167 mRNA. Translation: CAA48917.1. AE014297 Genomic DNA. Translation: AAF56125.1. AE014297 Genomic DNA. Translation: AAN13942.1. BT024187 mRNA. Translation: ABC86249.1. BT058033 mRNA. Translation: ACM16754.1. BT001893 mRNA. Translation: AAN71682.1. Sequence problems. M88472 mRNA. Translation: AAC34200.1.
PIR	S33168.
RefSeq	NP_524461.2. NM_079737.2. NP_732857.1. NM_170651.3.
UniGene	Dm.2370.
3D structure databases
ProteinModelPortal	P51023.
SMR	P51023. Positions 144-252, 587-716.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-19576N.
IntAct	P51023. 1 interaction.
MINT	MINT-2737387.
STRING	P51023.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0089717; FBpp0088658; FBgn0003118.
GeneID	42757.
KEGG	dme:Dmel_CG17077.
Organism-specific databases
CTD	42757.
FlyBase	FBgn0003118. pnt.
Phylogenomic databases
GeneTree	EMGT00050000000490.
InParanoid	P51023.
PhylomeDB	P51023.
Gene expression databases
ArrayExpress	P51023.
Bgee	P51023.
GermOnline	CG17077. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000418. Ets. IPR003118. Pointed_dom. IPR013761. SAM/pointed. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.150.50. SAM_type. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K02678.
Pfam	PF00178. Ets. 1 hit. PF02198. SAM_PNT. 1 hit. [Graphical view]
PRINTS	PR00454. ETSDOMAIN.
SMART	SM00413. ETS. 1 hit. SM00251. SAM_PNT. 1 hit. [Graphical view]
SUPFAM	SSF47769. SAM_homology. 1 hit.
PROSITE	PS00345. ETS_DOMAIN_1. 1 hit. PS00346. ETS_DOMAIN_2. 1 hit. PS50061. ETS_DOMAIN_3. 1 hit. PS51433. PNT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	830419.

Entry information

Entry name

PNT2_DROME

Accession

Primary (citable) accession number: P51023
Secondary accession number(s): B9EQV8 , Q29R53, Q8IG92, Q8IMZ1, Q9VCN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 16, 2001
Last modified:	January 25, 2012
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project