P51022 (PNT1_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ETS-like protein pointed, isoform P1
Short name=D-ETS-2

Gene names

Name:	pnt
Synonyms:	ETS2, Ets58AB
ORF Names:	CG17077

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required for glial-neuronal cell interactions at the ventral midline which are necessary for the proper elaboration of commissures in the embryonic CNS.
Subcellular location	Nucleus Potential.
Tissue specificity	Expressed in a complex dynamic pattern in early embryos, including the midline and midline glial cells. Ref.1 Ref.4
Developmental stage	Expressed throughout development with lower levels during larval development. Ref.4
Sequence similarities	Belongs to the ETS family. Contains 1 ETS DNA-binding domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Uncategorized	? Inferred from mutant phenotype. Source: FlyBase
Biological process	R3/R4 cell fate commitment Inferred from mutant phenotype. Source: FlyBase R7 cell development Traceable author statement. Source: FlyBase Ras protein signal transduction Traceable author statement. Source: FlyBase anti-apoptosis Inferred from genetic interaction. Source: FlyBase determination of genital disc primordium Inferred from mutant phenotype. Source: FlyBase epidermal growth factor receptor signaling pathway Non-traceable author statement. Source: FlyBase epithelial cell migration, open tracheal system Inferred from mutant phenotype. Source: FlyBase eye-antennal disc morphogenesis Inferred from mutant phenotype. Source: FlyBase imaginal disc-derived wing morphogenesis Inferred from mutant phenotype. Source: FlyBase muscle fiber development Inferred from mutant phenotype. Source: FlyBase negative regulation of cardioblast cell fate specification Inferred from mutant phenotype. Source: FlyBase ovarian follicle cell development Traceable author statement. Source: FlyBase peripheral nervous system development Traceable author statement. Source: FlyBase phagocytosis, engulfment Inferred from mutant phenotype. Source: FlyBase positive regulation of cell proliferation Inferred from mutant phenotype. Source: FlyBase primary branching, open tracheal system Inferred from mutant phenotype. Source: FlyBase regulation of hemocyte differentiation Inferred from mutant phenotype. Source: FlyBase secondary branching, open tracheal system Inferred from mutant phenotype. Source: FlyBase terminal region determination Inferred from genetic interaction. Source: FlyBase torso signaling pathway Inferred from genetic interaction. Source: FlyBase tracheal outgrowth, open tracheal system Inferred from mutant phenotype. Source: FlyBase wing disc dorsal/ventral pattern formation Inferred from genetic interaction. Source: FlyBase
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: FlyBase sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform P1 (identifier: P51022-1)

Also known as: C;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform D (identifier: P51023-2)

The sequence of this isoform can be found in the external entry P51023.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Isoform P2 (identifier: P51023-1)

Also known as: B;

The sequence of this isoform can be found in the external entry P51023.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 623	623	ETS-like protein pointed, isoform P1		PRO_0000204129
Regions
	DNA binding	515 – 595	81	ETS
	Compositional bias	38 – 46	9	Poly-Ser
	Compositional bias	47 – 50	4	Poly-Asn
	Compositional bias	75 – 88	14	Poly-His
	Compositional bias	103 – 111	9	Poly-Gln
	Compositional bias	119 – 124	6	Poly-Gln
	Compositional bias	128 – 134	7	Poly-Gln
	Compositional bias	156 – 159	4	Poly-Thr
	Compositional bias	160 – 174	15	Poly-Ser
	Compositional bias	246 – 252	7	Poly-Gln
	Compositional bias	264 – 267	4	Poly-Asn
	Compositional bias	294 – 298	5	Poly-Asn
	Compositional bias	310 – 320	11	Poly-Asn
	Compositional bias	323 – 326	4	Poly-Ala
	Compositional bias	386 – 389	4	Poly-Gly
Experimental info
	Sequence conflict	467	1	Q → R in AAA28521. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform P1 (C) [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F47083D860EB6480	FASTA	623	66,866
	10 20 30 40 50 60 MPPSAFLVNA SIISAANALD SDKEQQLQEH LNQSHFTSSS SSSNSSNNNN CHTAPNYNMV 70 80 90 100 110 120 LQSYENYPSY HLAQHHHHHH HHHSHPHHQL AQQTGLHSHH TMQQQLQQQQ QSLLLQHPQQ 130 140 150 160 170 180 QQQQHSHQSQ QQQQHGYGSS AQLPHHRLSG GSTGSTTTTS SGSSSSGSSS SASSQGFANG 190 200 210 220 230 240 SATANNLVGA LSSSTAASLG LGYFNDMAPF VGDANAYYTD SDVNFFSSGY NTSNTHDRIN 250 260 270 280 290 300 NSTPPQQQQS QQPTVNGSGS ASSNNNNSML PPAVQQSNNE NNNTSSSNTN NSSNNNNNSG 310 320 330 340 350 360 GSNNSNAGSN NNNNNNNNIN FMAAAAIFQH HLKEEPGTQN GNIGGYGGGS NSQNDPTDLS 370 380 390 400 410 420 SYGLPAHLAA YGGGSGSGPT GGRSSGGGGD ESDYHSTISA QDHQSQQSSG GNGSGGASGG 430 440 450 460 470 480 STGNSNGYLD SSSEFYGSYA GRNRFHDGYP PEFTPYDAQS FQSMGPQPTA MDQWGAAHAH 490 500 510 520 530 540 QHPAAYMSTL GLDKGLLGGY TTQGGVPCFT GSGPIQLWQF LLELLLDKTC QSFISWTGDG 550 560 570 580 590 600 WEFKLTDPDE VARRWGIRKN KPKMNYEKLS RGLRYYYDKN IIHKTAGKRY VYRFVCDLQN 610 620 LVGHTPEELV AKYDLKIEKK DVD
	Isoform D [UniParc]. See P51023.		–	–
	Isoform P2 (B) [UniParc]. See P51023.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila gene pointed encodes two ETS-like proteins which are involved in the development of the midline glial cells." Klaembt C. Development 117:163-176(1993) [PubMed: 8223245] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM P1), ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[4]	"The Drosophila ets-2 gene: molecular structure, chromosomal localization, and developmental expression." Pribyl L.J., Watson D.K., McWilliams M.J., Ascione R., Papas T.S. Dev. Biol. 127:45-53(1988) [PubMed: 2834248] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 445-603, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[5]	"Isolation and characterization of five Drosophila genes that encode an ets-related DNA binding domain." Chen T., Bunting M., Karim F.D., Thummel C.S. Dev. Biol. 151:176-191(1992) [PubMed: 1577186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 456-613.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69166 mRNA. Translation: CAA48916.1. AE014297 Genomic DNA. Translation: AAN13943.1. M20408 Genomic DNA. Translation: AAA28521.1. M88472 mRNA. Translation: AAC34200.1.
PIR	S33167. S33168.
RefSeq	NP_732858.1. NM_170070.1.
UniGene	Dm.2370.
3D structure databases
ProteinModelPortal	P51022.
SMR	P51022. Positions 509-595.
ModBase	Search...
Protein-protein interaction databases
STRING	P51022.
Proteomic databases
PRIDE	P51022.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0089715; FBpp0088656; FBgn0003118.
GeneID	42757.
KEGG	dme:Dmel_CG17077.
NMPDR	fig|7227.3.peg.14222.
Organism-specific databases
CTD	42757.
FlyBase	FBgn0003118. pnt.
Phylogenomic databases
eggNOG	inNOG09594.
GeneTree	EMGT00050000000490.
InParanoid	P51022.
OMA	NAYYTDS.
OrthoDB	EOG4VHHPJ.
Gene expression databases
Bgee	P51022.
GermOnline	CG17077. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000418. Ets. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K02678.
Pfam	PF00178. Ets. 1 hit. [Graphical view]
PRINTS	PR00454. ETSDOMAIN.
SMART	SM00413. ETS. 1 hit. [Graphical view]
PROSITE	PS00345. ETS_DOMAIN_1. 1 hit. PS00346. ETS_DOMAIN_2. 1 hit. PS50061. ETS_DOMAIN_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	830419.

Entry information

Entry name

PNT1_DROME

Accession

Primary (citable) accession number: P51022
Secondary accession number(s): P19420

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project