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P51020

- HOA_ECOLI

UniProt

P51020 - HOA_ECOLI

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Protein

4-hydroxy-2-oxovalerate aldolase

Gene

mhpE

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the retro-aldol cleavage of 4-hydroxy-2-oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of 3-phenylpropanoate.1 Publication

Catalytic activityi

(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate.

pH dependencei

Optimum pH is 6.25-6.75.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei14 – 141Transition state stabilizerBy similarity
Metal bindingi15 – 151ManganeseBy similarity
Active sitei18 – 181Proton acceptorSequence Analysis
Binding sitei168 – 1681SubstrateBy similarity
Metal bindingi197 – 1971Manganese; via tele nitrogenBy similarity
Binding sitei197 – 1971SubstrateBy similarity
Metal bindingi199 – 1991Manganese; via tele nitrogenBy similarity
Binding sitei288 – 2881SubstrateBy similarity

GO - Molecular functioni

  1. 4-hydroxy-2-oxovalerate aldolase activity Source: EcoCyc
  2. manganese ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 3-phenylpropionate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:MHPELY-MONOMER.
ECOL316407:JW0343-MONOMER.
MetaCyc:MHPELY-MONOMER.
UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-2-oxovalerate aldolase (EC:4.1.3.39)
Short name:
HOA
Alternative name(s):
4-hydroxy-2-keto-pentanoic acid aldolase
4-hydroxy-2-oxopentanoate aldolase
Gene namesi
Name:mhpE
Ordered Locus Names:b0352, JW0343
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13292. mhpE.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3373374-hydroxy-2-oxovalerate aldolasePRO_0000096470Add
BLAST

Proteomic databases

PaxDbiP51020.
PRIDEiP51020.

Expressioni

Gene expression databases

GenevestigatoriP51020.

Interactioni

Subunit structurei

Interacts with MhpF.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
mhpFP775801EBI-1116093,EBI-1116083

Protein-protein interaction databases

DIPiDIP-10209N.
IntActiP51020. 11 interactions.
STRINGi511145.b0352.

Structurei

3D structure databases

ProteinModelPortaliP51020.
SMRiP51020. Positions 4-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 152Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0119.
HOGENOMiHOG000048047.
InParanoidiP51020.
KOiK01666.
OMAiHEPDMIY.
OrthoDBiEOG6C2WC0.
PhylomeDBiP51020.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01656. HOA.
InterProiIPR017629. 4OH_2_O-val_aldolase.
IPR013785. Aldolase_TIM.
IPR012425. DmpG_comm.
IPR000891. PYR_CT.
[Graphical view]
PANTHERiPTHR10277:SF3. PTHR10277:SF3. 1 hit.
PfamiPF07836. DmpG_comm. 1 hit.
PF00682. HMGL-like. 1 hit.
[Graphical view]
ProDomiPD005364. DmpG_comm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITEiPS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51020-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNGKKLYISD VTLRDGMHAI RHQYSLENVR QIAKALDDAR VDSIEVAHGD
60 70 80 90 100
GLQGSSFNYG FGAHSDLEWI EAAADVVKHA KIATLLLPGI GTIHDLKNAW
110 120 130 140 150
QAGARVVRVA THCTEADVSA QHIQYARELG MDTVGFLMMS HMTTPENLAK
160 170 180 190 200
QAKLMEGYGA TCIYVVDSGG AMNMSDIRDR FRALKAELKP ETQTGMHAHH
210 220 230 240 250
NLSLGVANSI AAVEEGCDRI DASLAGMGAG AGNAPLEVFI AAADKLGWQH
260 270 280 290 300
GTDLYALMDA ADDLVRPLQD RPVRVDRETL ALGYAGVYSS FLRHCETAAA
310 320 330
RYGLSAVDIL VELGKRRMVG GQEDMIVDVA LDLRNNK
Length:337
Mass (Da):36,470
Last modified:November 1, 1997 - v2
Checksum:iEDA263721721212F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86239 Genomic DNA. Translation: BAA13057.1.
U73857 Genomic DNA. Translation: AAB18076.1.
U00096 Genomic DNA. Translation: AAC73455.1.
AP009048 Genomic DNA. Translation: BAE76134.1.
D85613 Genomic DNA. No translation available.
PIRiH64762.
RefSeqiNP_414886.1. NC_000913.3.
YP_488646.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73455; AAC73455; b0352.
BAE76134; BAE76134; BAE76134.
GeneIDi12932630.
945012.
KEGGiecj:Y75_p0341.
eco:b0352.
PATRICi32115837. VBIEscCol129921_0360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86239 Genomic DNA. Translation: BAA13057.1 .
U73857 Genomic DNA. Translation: AAB18076.1 .
U00096 Genomic DNA. Translation: AAC73455.1 .
AP009048 Genomic DNA. Translation: BAE76134.1 .
D85613 Genomic DNA. No translation available.
PIRi H64762.
RefSeqi NP_414886.1. NC_000913.3.
YP_488646.1. NC_007779.1.

3D structure databases

ProteinModelPortali P51020.
SMRi P51020. Positions 4-333.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-10209N.
IntActi P51020. 11 interactions.
STRINGi 511145.b0352.

Proteomic databases

PaxDbi P51020.
PRIDEi P51020.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73455 ; AAC73455 ; b0352 .
BAE76134 ; BAE76134 ; BAE76134 .
GeneIDi 12932630.
945012.
KEGGi ecj:Y75_p0341.
eco:b0352.
PATRICi 32115837. VBIEscCol129921_0360.

Organism-specific databases

EchoBASEi EB3077.
EcoGenei EG13292. mhpE.

Phylogenomic databases

eggNOGi COG0119.
HOGENOMi HOG000048047.
InParanoidi P51020.
KOi K01666.
OMAi HEPDMIY.
OrthoDBi EOG6C2WC0.
PhylomeDBi P51020.

Enzyme and pathway databases

UniPathwayi UPA00714 .
BioCyci EcoCyc:MHPELY-MONOMER.
ECOL316407:JW0343-MONOMER.
MetaCyc:MHPELY-MONOMER.

Miscellaneous databases

PROi P51020.

Gene expression databases

Genevestigatori P51020.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01656. HOA.
InterProi IPR017629. 4OH_2_O-val_aldolase.
IPR013785. Aldolase_TIM.
IPR012425. DmpG_comm.
IPR000891. PYR_CT.
[Graphical view ]
PANTHERi PTHR10277:SF3. PTHR10277:SF3. 1 hit.
Pfami PF07836. DmpG_comm. 1 hit.
PF00682. HMGL-like. 1 hit.
[Graphical view ]
ProDomi PD005364. DmpG_comm. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
TIGRFAMsi TIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITEi PS50991. PYR_CT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence of the mhp operon."
    Kawamukai M.
    Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. Nashimoto H., Saito N.
    Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-337.
    Strain: K12.
  6. "Substrate selectivity and biochemical properties of 4-hydroxy-2-keto-pentanoic acid aldolase from Escherichia coli."
    Pollard J.R., Rialland D., Bugg T.D.
    Appl. Environ. Microbiol. 64:4093-4094(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, FUNCTION, SUBSTRATE SPECIFICITY.
  7. "Coupled expression of MhpE aldolase and MhpF dehydrogenase in Escherichia coli."
    Lee S.J., Ko J.H., Kang H.Y., Lee Y.
    Biochem. Biophys. Res. Commun. 346:1009-1015(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MHPF.

Entry informationi

Entry nameiHOA_ECOLI
AccessioniPrimary (citable) accession number: P51020
Secondary accession number(s): P77787, Q2MC72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Presumably stereoselective for the 4S-enantiomer of 4-hydroxy-2-ketovalerate.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3