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Protein

4-hydroxy-2-oxovalerate aldolase

Gene

mhpE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the retro-aldol cleavage of 4-hydroxy-2-oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of 3-phenylpropanoate.1 Publication

Miscellaneous

Presumably stereoselective for the 4S-enantiomer of 4-hydroxy-2-ketovalerate.

Catalytic activityi

(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate.

pH dependencei

Optimum pH is 6.25-6.75.1 Publication

Pathwayi: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei14Transition state stabilizerBy similarity1
Metal bindingi15ManganeseBy similarity1
Active sitei18Proton acceptorSequence analysis1
Binding sitei168SubstrateBy similarity1
Metal bindingi197Manganese; via tele nitrogenBy similarity1
Binding sitei197SubstrateBy similarity1
Metal bindingi199Manganese; via tele nitrogenBy similarity1
Binding sitei288SubstrateBy similarity1

GO - Molecular functioni

  • 4-hydroxy-2-oxovalerate aldolase activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAromatic hydrocarbons catabolism
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:MHPELY-MONOMER
MetaCyc:MHPELY-MONOMER
UniPathwayiUPA00714

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-2-oxovalerate aldolase (EC:4.1.3.39)
Short name:
HOA
Alternative name(s):
4-hydroxy-2-keto-pentanoic acid aldolase
4-hydroxy-2-oxopentanoate aldolase
Gene namesi
Name:mhpE
Ordered Locus Names:b0352, JW0343
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13292 mhpE

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000964701 – 3374-hydroxy-2-oxovalerate aldolaseAdd BLAST337

Proteomic databases

PaxDbiP51020
PRIDEiP51020

Interactioni

Subunit structurei

Interacts with MhpF.1 Publication

Protein-protein interaction databases

BioGridi4261624, 13 interactors
DIPiDIP-10209N
IntActiP51020, 11 interactors
STRINGi316407.85674494

Structurei

3D structure databases

ProteinModelPortaliP51020
SMRiP51020
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 258Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST253

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 15Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DF3 Bacteria
COG0119 LUCA
HOGENOMiHOG000048047
InParanoidiP51020
KOiK01666
OMAiFLMMAHS
PhylomeDBiP51020

Family and domain databases

CDDicd07943 DRE_TIM_HOA, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01656 HOA, 1 hit
InterProiView protein in InterPro
IPR017629 4OH_2_O-val_aldolase
IPR013785 Aldolase_TIM
IPR012425 DmpG_comm
IPR035685 DRE_TIM_HOA
IPR000891 PYR_CT
PfamiView protein in Pfam
PF07836 DmpG_comm, 1 hit
PF00682 HMGL-like, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD005364 DmpG_comm, 1 hit
TIGRFAMsiTIGR03217 4OH_2_O_val_ald, 1 hit
PROSITEiView protein in PROSITE
PS50991 PYR_CT, 1 hit

Sequencei

Sequence statusi: Complete.

P51020-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGKKLYISD VTLRDGMHAI RHQYSLENVR QIAKALDDAR VDSIEVAHGD
60 70 80 90 100
GLQGSSFNYG FGAHSDLEWI EAAADVVKHA KIATLLLPGI GTIHDLKNAW
110 120 130 140 150
QAGARVVRVA THCTEADVSA QHIQYARELG MDTVGFLMMS HMTTPENLAK
160 170 180 190 200
QAKLMEGYGA TCIYVVDSGG AMNMSDIRDR FRALKAELKP ETQTGMHAHH
210 220 230 240 250
NLSLGVANSI AAVEEGCDRI DASLAGMGAG AGNAPLEVFI AAADKLGWQH
260 270 280 290 300
GTDLYALMDA ADDLVRPLQD RPVRVDRETL ALGYAGVYSS FLRHCETAAA
310 320 330
RYGLSAVDIL VELGKRRMVG GQEDMIVDVA LDLRNNK
Length:337
Mass (Da):36,470
Last modified:November 1, 1997 - v2
Checksum:iEDA263721721212F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86239 Genomic DNA Translation: BAA13057.1
U73857 Genomic DNA Translation: AAB18076.1
U00096 Genomic DNA Translation: AAC73455.1
AP009048 Genomic DNA Translation: BAE76134.1
D85613 Genomic DNA No translation available.
PIRiH64762
RefSeqiNP_414886.1, NC_000913.3
WP_001013499.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73455; AAC73455; b0352
BAE76134; BAE76134; BAE76134
GeneIDi945012
KEGGiecj:JW0343
eco:b0352
PATRICifig|1411691.4.peg.1926

Similar proteinsi

Entry informationi

Entry nameiHOA_ECOLI
AccessioniPrimary (citable) accession number: P51020
Secondary accession number(s): P77787, Q2MC72
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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