Reviewed, UniProtKB/Swiss-Prot P51020 (MHPE_ECOLI)

Names and origin

Protein names

Recommended name:
    4-hydroxy-2-oxovalerate aldolase
      Short name=HOA
    EC=4.1.3.39
Alternative name(s):
    4-hydroxy-2-keto-pentanoic acid aldolase

Gene names

Name:	mhpE
Synonyms:	mhpF
Ordered Locus Names:	b0352, JW0343

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the fission of 4-hydroxy-2-ketovalerate to yield pyruvate and acetaldehyde. Ref.6
Catalytic activity	4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate. HAMAP MF_01656
Pathway	Aromatic compound metabolism; 3-phenylpropionic acid degradation. HAMAP MF_01656
Subunit structure	Interacts with mhpF. Ref.7
Miscellaneous	Presumably stereoselective for the 4S-enantiomer of 4-hydroxy-2-ketovalerate. HAMAP MF_01656
Sequence similarities	Belongs to the mhpE aldolase family.
Biophysicochemical properties	pH dependence: Optimum pH is 6.25-6.75. HAMAP MF_01656

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-hydroxy-2-oxovalerate aldolase activity Inferred from electronic annotation. Source: HAMAP protein binding Ref.7 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
mhpF	P77580	1	EBI-1116093,EBI-1116083

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	4-hydroxy-2-oxovalerate aldolase HAMAP MF_01656		PRO_0000096470

Sequences

	Sequence		Length	Mass (Da)
	P51020-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: EDA263721721212F	FASTA	337	36,470
	10 20 30 40 50 60 MNGKKLYISD VTLRDGMHAI RHQYSLENVR QIAKALDDAR VDSIEVAHGD GLQGSSFNYG 70 80 90 100 110 120 FGAHSDLEWI EAAADVVKHA KIATLLLPGI GTIHDLKNAW QAGARVVRVA THCTEADVSA 130 140 150 160 170 180 QHIQYARELG MDTVGFLMMS HMTTPENLAK QAKLMEGYGA TCIYVVDSGG AMNMSDIRDR 190 200 210 220 230 240 FRALKAELKP ETQTGMHAHH NLSLGVANSI AAVEEGCDRI DASLAGMGAG AGNAPLEVFI 250 260 270 280 290 300 AAADKLGWQH GTDLYALMDA ADDLVRPLQD RPVRVDRETL ALGYAGVYSS FLRHCETAAA 310 320 330 RYGLSAVDIL VELGKRRMVG GQEDMIVDVA LDLRNNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Kawamukai M. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	Nashimoto H., Saito N. Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-337. Strain: K12.
[6]	"Substrate selectivity and biochemical properties of 4-hydroxy-2-keto-pentanoic acid aldolase from Escherichia coli." Pollard J.R., Rialland D., Bugg T.D. Appl. Environ. Microbiol. 64:4093-4094(1998) [PubMed: 9758851] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES, FUNCTION, SUBSTRATE SPECIFICITY.
[7]	"Coupled expression of MhpE aldolase and MhpF dehydrogenase in Escherichia coli." Lee S.J., Ko J.H., Kang H.Y., Lee Y. Biochem. Biophys. Res. Commun. 346:1009-1015(2006) [PubMed: 16782065] [Abstract] Cited for: INTERACTION WITH MHPF.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D86239 Genomic DNA. Translation: BAA13057.1. U73857 Genomic DNA. Translation: AAB18076.1. U00096 Genomic DNA. Translation: AAC73455.1. AP009048 Genomic DNA. Translation: BAE76134.1. D85613 Genomic DNA. No translation available.
PIR	H64762.
RefSeq	AP_001004.1. NP_414886.1.
3D structure databases
HSSP	HSSP built from PDB template 1NVM based on UniProtKB P51016.
SMR	P51020. Positions 4-333.
ModBase	Search...
Protein-protein interaction databases
IntAct	P51020. 10 interactions.
Genome annotation databases
GeneID	945012.
GenomeReviews	Gene locus JW0343 in contig AP009048_GR. Gene locus b0352 in contig U00096_GR.
KEGG	ecj:JW0343. eco:b0352.
Organism-specific databases
EchoBASE	EB3077.
EcoGene	EG13292. mhpE.
CMR	Search...
Phylogenomic databases
HOGENOM	P51020.
OMA	P51020. ATCVYVV.
Enzyme and pathway databases
BioCyc	EcoCyc:MHPELY-MON. MetaCyc:MHPELY-MON.
Family and domain databases
HAMAP	MF_01656. [Tree]
InterPro	IPR017629. 4OH_2_O-val_aldolase. IPR013785. Aldolase_TIM. IPR012425. DmpG_comm. IPR000891. PYR_CT. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF07836. DmpG_comm. 1 hit. PF00682. HMGL-like. 1 hit. [Graphical view]
TIGRFAMs	TIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITE	PS50991. PYR_CT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MHPE_ECOLI

Accession

Primary (citable) accession number: P51020
Secondary accession number(s): P77787, Q2MC72

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)