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Protein

4-hydroxy-2-oxovalerate aldolase

Gene

mhpE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the retro-aldol cleavage of 4-hydroxy-2-oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of 3-phenylpropanoate.1 Publication

Catalytic activityi

(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate.

pH dependencei

Optimum pH is 6.25-6.75.1 Publication

Pathwayi: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei14Transition state stabilizerBy similarity1
Metal bindingi15ManganeseBy similarity1
Active sitei18Proton acceptorSequence analysis1
Binding sitei168SubstrateBy similarity1
Metal bindingi197Manganese; via tele nitrogenBy similarity1
Binding sitei197SubstrateBy similarity1
Metal bindingi199Manganese; via tele nitrogenBy similarity1
Binding sitei288SubstrateBy similarity1

GO - Molecular functioni

  • 4-hydroxy-2-oxovalerate aldolase activity Source: EcoCyc
  • manganese ion binding Source: UniProtKB-HAMAP

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:MHPELY-MONOMER.
ECOL316407:JW0343-MONOMER.
MetaCyc:MHPELY-MONOMER.
UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-2-oxovalerate aldolase (EC:4.1.3.39)
Short name:
HOA
Alternative name(s):
4-hydroxy-2-keto-pentanoic acid aldolase
4-hydroxy-2-oxopentanoate aldolase
Gene namesi
Name:mhpE
Ordered Locus Names:b0352, JW0343
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13292. mhpE.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000964701 – 3374-hydroxy-2-oxovalerate aldolaseAdd BLAST337

Proteomic databases

PaxDbiP51020.
PRIDEiP51020.

Interactioni

Subunit structurei

Interacts with MhpF.1 Publication

Protein-protein interaction databases

BioGridi4261624. 11 interactors.
DIPiDIP-10209N.
IntActiP51020. 11 interactors.
STRINGi511145.b0352.

Structurei

3D structure databases

ProteinModelPortaliP51020.
SMRiP51020.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 258Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST253

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 15Substrate bindingBy similarity2

Sequence similaritiesi

Contains 1 pyruvate carboxyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105DF3. Bacteria.
COG0119. LUCA.
HOGENOMiHOG000048047.
InParanoidiP51020.
KOiK01666.
OMAiPETATGM.
PhylomeDBiP51020.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01656. HOA. 1 hit.
InterProiIPR017629. 4OH_2_O-val_aldolase.
IPR013785. Aldolase_TIM.
IPR012425. DmpG_comm.
IPR000891. PYR_CT.
[Graphical view]
PANTHERiPTHR10277:SF3. PTHR10277:SF3. 1 hit.
PfamiPF07836. DmpG_comm. 1 hit.
PF00682. HMGL-like. 1 hit.
[Graphical view]
ProDomiPD005364. DmpG_comm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITEiPS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51020-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGKKLYISD VTLRDGMHAI RHQYSLENVR QIAKALDDAR VDSIEVAHGD
60 70 80 90 100
GLQGSSFNYG FGAHSDLEWI EAAADVVKHA KIATLLLPGI GTIHDLKNAW
110 120 130 140 150
QAGARVVRVA THCTEADVSA QHIQYARELG MDTVGFLMMS HMTTPENLAK
160 170 180 190 200
QAKLMEGYGA TCIYVVDSGG AMNMSDIRDR FRALKAELKP ETQTGMHAHH
210 220 230 240 250
NLSLGVANSI AAVEEGCDRI DASLAGMGAG AGNAPLEVFI AAADKLGWQH
260 270 280 290 300
GTDLYALMDA ADDLVRPLQD RPVRVDRETL ALGYAGVYSS FLRHCETAAA
310 320 330
RYGLSAVDIL VELGKRRMVG GQEDMIVDVA LDLRNNK
Length:337
Mass (Da):36,470
Last modified:November 1, 1997 - v2
Checksum:iEDA263721721212F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86239 Genomic DNA. Translation: BAA13057.1.
U73857 Genomic DNA. Translation: AAB18076.1.
U00096 Genomic DNA. Translation: AAC73455.1.
AP009048 Genomic DNA. Translation: BAE76134.1.
D85613 Genomic DNA. No translation available.
PIRiH64762.
RefSeqiNP_414886.1. NC_000913.3.
WP_001013499.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73455; AAC73455; b0352.
BAE76134; BAE76134; BAE76134.
GeneIDi945012.
KEGGiecj:JW0343.
eco:b0352.
PATRICi32115837. VBIEscCol129921_0360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86239 Genomic DNA. Translation: BAA13057.1.
U73857 Genomic DNA. Translation: AAB18076.1.
U00096 Genomic DNA. Translation: AAC73455.1.
AP009048 Genomic DNA. Translation: BAE76134.1.
D85613 Genomic DNA. No translation available.
PIRiH64762.
RefSeqiNP_414886.1. NC_000913.3.
WP_001013499.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP51020.
SMRiP51020.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261624. 11 interactors.
DIPiDIP-10209N.
IntActiP51020. 11 interactors.
STRINGi511145.b0352.

Proteomic databases

PaxDbiP51020.
PRIDEiP51020.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73455; AAC73455; b0352.
BAE76134; BAE76134; BAE76134.
GeneIDi945012.
KEGGiecj:JW0343.
eco:b0352.
PATRICi32115837. VBIEscCol129921_0360.

Organism-specific databases

EchoBASEiEB3077.
EcoGeneiEG13292. mhpE.

Phylogenomic databases

eggNOGiENOG4105DF3. Bacteria.
COG0119. LUCA.
HOGENOMiHOG000048047.
InParanoidiP51020.
KOiK01666.
OMAiPETATGM.
PhylomeDBiP51020.

Enzyme and pathway databases

UniPathwayiUPA00714.
BioCyciEcoCyc:MHPELY-MONOMER.
ECOL316407:JW0343-MONOMER.
MetaCyc:MHPELY-MONOMER.

Miscellaneous databases

PROiP51020.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01656. HOA. 1 hit.
InterProiIPR017629. 4OH_2_O-val_aldolase.
IPR013785. Aldolase_TIM.
IPR012425. DmpG_comm.
IPR000891. PYR_CT.
[Graphical view]
PANTHERiPTHR10277:SF3. PTHR10277:SF3. 1 hit.
PfamiPF07836. DmpG_comm. 1 hit.
PF00682. HMGL-like. 1 hit.
[Graphical view]
ProDomiPD005364. DmpG_comm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITEiPS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHOA_ECOLI
AccessioniPrimary (citable) accession number: P51020
Secondary accession number(s): P77787, Q2MC72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Presumably stereoselective for the 4S-enantiomer of 4-hydroxy-2-ketovalerate.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.