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Protein

4-hydroxy-2-oxovalerate aldolase 1

Gene

todH

Organism
Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the retro-aldol cleavage of 4-hydroxy-2-oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds.UniRule annotation

Catalytic activityi

(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate.UniRule annotation

Pathwayi: toluene degradation

This protein is involved in the pathway toluene degradation, which is part of Xenobiotic degradation.
View all proteins of this organism that are known to be involved in the pathway toluene degradation and in Xenobiotic degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei14Transition state stabilizerUniRule annotation1
Metal bindingi15ManganeseUniRule annotation1
Active sitei18Proton acceptorUniRule annotation1
Binding sitei168SubstrateUniRule annotation1
Metal bindingi197Manganese; via tele nitrogenUniRule annotation1
Binding sitei197SubstrateUniRule annotation1
Metal bindingi199Manganese; via tele nitrogenUniRule annotation1
Binding sitei288SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11384.
UniPathwayiUPA00273.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-2-oxovalerate aldolase 1UniRule annotation (EC:4.1.3.39UniRule annotation)
Short name:
HOA 1UniRule annotation
Alternative name(s):
4-hydroxy-2-keto-pentanoic acid aldolase 1UniRule annotation
4-hydroxy-2-oxopentanoate aldolase 1UniRule annotation
Gene namesi
Name:todH
Ordered Locus Names:Pput_2873
OrganismiPseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)
Taxonomic identifieri351746 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000006553 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000726181 – 3524-hydroxy-2-oxovalerate aldolase 1Add BLAST352

Interactioni

Protein-protein interaction databases

STRINGi351746.Pput_2873.

Structurei

3D structure databases

ProteinModelPortaliP51018.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 258Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST253

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 15Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the 4-hydroxy-2-oxovalerate aldolase family.UniRule annotation
Contains 1 pyruvate carboxyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105DF3. Bacteria.
COG0119. LUCA.
HOGENOMiHOG000048047.
KOiK01666.
OMAiPETATGM.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01656. HOA. 1 hit.
InterProiIPR017629. 4OH_2_O-val_aldolase.
IPR013785. Aldolase_TIM.
IPR012425. DmpG_comm.
IPR000891. PYR_CT.
[Graphical view]
PANTHERiPTHR10277:SF3. PTHR10277:SF3. 1 hit.
PfamiPF07836. DmpG_comm. 1 hit.
PF00682. HMGL-like. 1 hit.
[Graphical view]
ProDomiPD005364. DmpG_comm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITEiPS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51018-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQQKLYISD VTLRDGSHAI RHQYTVEQVK QIARALDDAK VDSIEVAHGD
60 70 80 90 100
GLQGGSFNYG FGAHTDLEWI EAAASVVKHA KIATLLLPGI GTVHDLKAVY
110 120 130 140 150
EAGVRVVRVA THCTEADISR QHIEYARHLG MEAVGFLMMS HMTTPQHLAQ
160 170 180 190 200
QAKLMESYGA TVCYVVDSGG ALSMNDVRDR FRAFKDVLKP ETQTGMHAHH
210 220 230 240 250
NLSLGVANSI VAVENGCDRV DASLAGMGAG AGNAPLEVFI AAAERMGWNH
260 270 280 290 300
GTDLYKLMDA ADDLVRPLQD RPVRVDRETL ALGYAGVYSS FLRHSEMAAS
310 320 330 340 350
KYGLKTVDIL VELGRRRMVG GQEDMIIDVA LDLLKQQEHE RIRSEPVSSE

AN
Length:352
Mass (Da):38,474
Last modified:January 15, 2008 - v2
Checksum:i967194FA65C4441A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64 – 65HT → QS in AAA61944 (PubMed:8063106).Curated2
Sequence conflicti79 – 80HA → AS in AAA61944 (PubMed:8063106).Curated2
Sequence conflicti341R → G in AAA61944 (PubMed:8063106).Curated1
Sequence conflicti349S → T in AAA61944 (PubMed:8063106).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09250 Genomic DNA. Translation: AAA61944.1.
CP000712 Genomic DNA. Translation: ABQ79004.1.
RefSeqiWP_012052593.1. NC_009512.1.

Genome annotation databases

EnsemblBacteriaiABQ79004; ABQ79004; Pput_2873.
KEGGippf:Pput_2873.
PATRICi19921889. VBIPsePut56420_2929.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09250 Genomic DNA. Translation: AAA61944.1.
CP000712 Genomic DNA. Translation: ABQ79004.1.
RefSeqiWP_012052593.1. NC_009512.1.

3D structure databases

ProteinModelPortaliP51018.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351746.Pput_2873.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ79004; ABQ79004; Pput_2873.
KEGGippf:Pput_2873.
PATRICi19921889. VBIPsePut56420_2929.

Phylogenomic databases

eggNOGiENOG4105DF3. Bacteria.
COG0119. LUCA.
HOGENOMiHOG000048047.
KOiK01666.
OMAiPETATGM.

Enzyme and pathway databases

UniPathwayiUPA00273.
BioCyciMetaCyc:MONOMER-11384.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01656. HOA. 1 hit.
InterProiIPR017629. 4OH_2_O-val_aldolase.
IPR013785. Aldolase_TIM.
IPR012425. DmpG_comm.
IPR000891. PYR_CT.
[Graphical view]
PANTHERiPTHR10277:SF3. PTHR10277:SF3. 1 hit.
PfamiPF07836. DmpG_comm. 1 hit.
PF00682. HMGL-like. 1 hit.
[Graphical view]
ProDomiPD005364. DmpG_comm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITEiPS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHOA1_PSEP1
AccessioniPrimary (citable) accession number: P51018
Secondary accession number(s): A5W4E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 15, 2008
Last modified: November 2, 2016
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.