Reviewed, UniProtKB/Swiss-Prot P51017 (NAHM_PSEPU)

Names and origin

Protein names

Recommended name:
    4-hydroxy-2-oxovalerate aldolase
      Short name=HOA
    EC=4.1.3.39

Gene names

Name:

nahM

Encoded on

Plasmid pWW60-22

Organism

Pseudomonas putida

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Catalyzes the production of pyruvate and acetaldehyde from 4-hydroxy-2-oxovalerate.
Catalytic activity	4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate.
Pathway	Aromatic compound metabolism; salicylic acid degradation.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Molecular function	Lyase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-hydroxy-2-oxovalerate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	4-hydroxy-2-oxovalerate aldolase		PRO_0000096706

Sequences

	Sequence		Length	Mass (Da)
	P51017-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 65963EE93E4BAB7F	FASTA	346	37,102
	10 20 30 40 50 60 MNLHGKSVIL HDMSLRDGMH AKRHQISLEQ MVAVATGLDQ AGMPLIEITH GDGLGGRSIN 70 80 90 100 110 120 YGFPAHSDEE YLRAVIPQLK QAKVSALLLP GIGTVDHLKM ALDCGVSTIR VATHCTEADV 130 140 150 160 170 180 SEQHIGMARK LGVDTVGFLM MAHMISAEKV LEQAKLMESY GANCIYCTDS AGYMLPDEVS 190 200 210 220 230 240 EKIGLLRAEL NPATEVGFHG HHNMGMAIAN SLAAIEAGAA RIDGSVAGLG AGAGNTPLEV 250 260 270 280 290 300 FVAVCKRMGV ETGIDLYKIM DVAEDLVVPM MDQPIRVDRD ALTLGYAGVY SSFLLFAQRA 310 320 330 340 EKKYGVSARD ILVELGRRGT VGGQEDMIED LALDMARARQ QQKVSA

References

[1]

"The 4-hydroxy-2-oxovalerate aldolase and acetaldehyde dehydrogenase (acylating) encoded by the nahM and nahO genes of the naphthalene catabolic plasmid pWW60-22 provide further evidence of conservation of meta-cleavage pathway gene sequences." Platt A., Shingler V., Taylor S.C., Williams P.A. Microbiology 141:2223-2233(1995) [PubMed: 7496535] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCIMB 9816.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U13232 Genomic DNA. Translation: AAA89107.1.
RefSeq	NP_863098.1.
3D structure databases
HSSP	HSSP built from PDB template 1NVM based on UniProtKB P51016.
ModBase	Search...
Genome annotation databases
GeneID	1343200.
Enzyme and pathway databases
BRENDA	4.1.3.39. 403.
Family and domain databases
InterPro	IPR017629. 4OH_2_O-val_aldolase. IPR013785. Aldolase_TIM. IPR012425. DmpG_comm. IPR000891. PYR_CT. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF07836. DmpG_comm. 1 hit. PF00682. HMGL-like. 1 hit. [Graphical view]
TIGRFAMs	TIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITE	PS50991. PYR_CT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAHM_PSEPU

Accession

Primary (citable) accession number: P51017

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)