Reviewed, UniProtKB/Swiss-Prot P51016 (DMPG_PSEUF)

Names and origin

Protein names

Recommended name:
    4-hydroxy-2-oxovalerate aldolase
      Short name=HOA
    EC=4.1.3.-

Gene names

Name:

dmpG

Encoded on

Plasmid pVI150

Organism

Pseudomonas sp. (strain CF600)

Taxonomic identifier

79676 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	4-hydroxy-2-oxovalerate = pyruvate + acetaldehyde.
Pathway	Aromatic compound metabolism; benzoic acid degradation via hydroxylation.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Molecular function	Lyase
Technical term	3D-structure Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-hydroxy-2-oxovalerate aldolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 345

345

4-hydroxy-2-oxovalerate aldolase

PRO_0000079938

Secondary structure

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345

Helix Strand Turn

Details...

Beta strand

10 – 13

4

Turn

15 – 17

3

Helix

18 – 22

5

Turn

23 – 25

3

Helix

29 – 42

14

Beta strand

45 – 48

4

Turn

60 – 62

3

Helix

69 – 77

9

Beta strand

81 – 89

9

Helix

96 – 105

10

Beta strand

109 – 115

7

Helix

119 – 122

4

Helix

123 – 132

10

Beta strand

135 – 142

8

Helix

148 – 161

14

Beta strand

164 – 169

6

Helix

177 – 190

14

Beta strand

195 – 200

6

Helix

208 – 217

10

Beta strand

222 – 226

5

Helix

227 – 229

3

Helix

239 – 249

11

Helix

257 – 266

10

Helix

269 – 271

3

Helix

280 – 288

9

Helix

294 – 305

12

Helix

309 – 319

11

Helix

327 – 340

14

Sequences

	Sequence		Length	Mass (Da)
	P51016-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 8B38B37A8A0EFEE0	FASTA	345	37,471
	10 20 30 40 50 60 MTFNPSKKLY ISDVTLRDGS HAIRHQYTLD DVRAIARALD KAKVDSIEVA HGDGLQGSSF 70 80 90 100 110 120 NYGFGRHTDL EYIEAVAGEI SHAQIATLLL PGIGSVHDLK NAYQAGARVV RVATHCTEAD 130 140 150 160 170 180 VSKQHIEYAR NLGMDTVGFL MMSHMIPAEK LAEQGKLMES YGATCIYMAD SGGAMSMNDI 190 200 210 220 230 240 RDRMRAFKAV LKPETQVGMH AHHNLSLGVA NSIVAVEEGC DRVDASLAGM GAGAGNAPLE 250 260 270 280 290 300 VFIAVAERLG WNHGTDLYTL MDAADDIVRP LQDRPVRVDR ETLGLGYAGV YSSFLRHAEI 310 320 330 340 AAAKYNLKTL DILVELGHRR MVGGQEDMIV DVALDLLAAH KENRA

References

[1]	"Nucleotide sequence and functional analysis of the complete phenol/3,4-dimethylphenol catabolic pathway of Pseudomonas sp. strain CF600." Shingler V., Marklund U., Powlowski J. J. Bacteriol. 174:711-724(1992) [PubMed: 1732207] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBLGenBankDDBJ

X60835 Genomic DNA. Translation: CAA43227.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NVM	X-ray	1.70	A/C/E/G	1-345	[»]

ModBase

Search...

Enzyme and pathway databases

BioCyc

MetaCyc:MON-12778.

Family and domain databases

InterPro

IPR017629. 4OH_2_O-val_aldolase.
IPR013785. Aldolase_TIM.
IPR012425. DmpG_comm.
IPR000891. PYR_CT.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

Pfam

PF07836. DmpG_comm. 1 hit.
PF00682. HMGL-like. 1 hit.
[Graphical view]

TIGRFAMs

TIGR03217. 4OH_2_O_val_ald. 1 hit.

PROSITE

PS50991. PYR_CT. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DMPG_PSEUF

Accession

Primary (citable) accession number: P51016

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)