P51011 (LDHD_LEUMC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: D-lactate dehydrogenase Short name=D-LDH EC=1.1.1.28 Alternative name(s): D-specific D-2-hydroxyacid dehydrogenase
Organism	Leuconostoc mesenteroides subsp. cremoris
Taxonomic identifier	33965 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Lactobacillales › Leuconostoc

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-lactate + NAD+ = pyruvate + NADH.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Molecular function	D-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	D-lactate dehydrogenase		PRO_0000075957
Regions
	Nucleotide binding	155 – 156	2	NAD By similarity
	Nucleotide binding	233 – 235	3	NAD By similarity
	Nucleotide binding	296 – 299	4	NAD By similarity
Sites
	Active site	235	1	By similarity
	Active site	264	1	By similarity
	Active site	296	1	Proton donor By similarity
	Binding site	259	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51011 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 0A63C462303672D8	FASTA	331	36,316
	10 20 30 40 50 60 MKIFAYGIRD DEKPSLEEWK AANPEIEVDY TQELLTPETA KLAEGSDSAV VYQQLDYTRE 70 80 90 100 110 120 TLTALANVGV TNLSLRNVGT DNIDFDAARE FNFNISNVPV YSPNAIAEHS MLQLSRLLRR 130 140 150 160 170 180 TKALDAKIAK RDLRWAPTTG REMRMQTVGV IGTGHIGRVA INILKGFGAK VIAYDKYPNA 190 200 210 220 230 240 ELQAEGLYVD TLDELYAQAD AISLYVPGVP ENHHLINADA IAKMKDGVVI MNAARGNLMD 250 260 270 280 290 300 IDAIIDGLNS GKISDFGMDV YENEVACSMK IGLVKNSPDA KIADLIAREN VMITPHTAFY 310 320 330 TTKAVLEMVH QSFDAAVAFA KGEKPAIAVE Y

References

[1]	"Purification, properties and DNA sequence of the D-lactate dehydrogenase from Leuconostoc mesenteroides subsp. cremoris." Dartois V., Phalip V., Schmitt P., Divies C. Res. Microbiol. 146:291-302(1995) [PubMed: 7569323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 195.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L29327 Genomic DNA. Translation: AAA99506.1.
3D structure databases
ProteinModelPortal	P51011.
SMR	P51011. Positions 2-325.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13061.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHD_LEUMC

Accession

Primary (citable) accession number: P51011

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 28, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program