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Protein

Poly(A) polymerase alpha-A

Gene

papola-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor (CPSF).

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions. Also active with manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei91ATPBy similarity1
Metal bindingi95Magnesium 1; catalyticBy similarity1
Metal bindingi95Magnesium 2; catalyticBy similarity1
Metal bindingi97Magnesium 1; catalyticBy similarity1
Metal bindingi97Magnesium 2; catalyticBy similarity1
Metal bindingi149Magnesium 2; catalyticBy similarity1
Binding sitei149ATPBy similarity1
Binding sitei210ATPBy similarity1
Binding sitei219ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi82 – 84ATPBy similarity3
Nucleotide bindingi95 – 97ATPBy similarity3
Nucleotide bindingi228 – 229ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase alpha-A (EC:2.7.7.19)
Short name:
PAP-alpha-A
Alternative name(s):
Polynucleotide adenylyltransferase alpha-A
Gene namesi
Name:papola-a
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000051614‹1 – 715Poly(A) polymerase alpha-AAdd BLAST›715

Proteomic databases

PRIDEiP51004.

Interactioni

Subunit structurei

Monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei135Interaction with RNABy similarity1
Sitei140Interaction with RNABy similarity1
Sitei310Interaction with RNABy similarity1
Sitei381Interaction with RNABy similarity1
Sitei506Interaction with RNABy similarity1

Structurei

3D structure databases

ProteinModelPortaliP51004.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi472 – 489Nuclear localization signal 1By similarityAdd BLAST18
Motifi624 – 639Nuclear localization signal 2By similarityAdd BLAST16

Sequence similaritiesi

Belongs to the poly(A) polymerase family.Curated

Phylogenomic databases

HOVERGENiHBG053502.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR10682. PTHR10682. 1 hit.
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Fragment.

P51004-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
PKTFGITSPI SLAAAKDTDC TLMQKLIETL KPYGVFEEEE QLQHRILGKL
60 70 80 90 100
NNLVKEWIRE ISELKHLPQS VIENVGGKIF TFGPYRLGVH TKGADIDALC
110 120 130 140 150
VAPRHVDRSD FFSSFYDKLK QQEEVKDLRS VEEAFVPVIK LCFDGIEIDI
160 170 180 190 200
LFARLALQTI PEDLDLRDDS LLKNLDIRCI RSLNGCRVTD EILHLVPNID
210 220 230 240 250
SFRLTLRAIK LWAKRHNIYS NILGFLGGVS WAMLVARTCQ LYPNAIASTL
260 270 280 290 300
VHKFFLVFSK WEWPNPVLLK QPEECNLNLP VWDPRVNPSD RYHLMPIITP
310 320 330 340 350
AYPQQNSTYN VSVSTRAVMI EEFKQGLAIT DEILLGKAEW SKLFDAPNFF
360 370 380 390 400
QKYKHYILLL ASAPTEKQRL EWVGLVESKI RILVGSLEKN EFITLAHVNP
410 420 430 440 450
RSFPAPSENM EKEEFRTMWV IGLVFKKMEN SENLSVDLTY DIQSFTDTVD
460 470 480 490 500
RQAINSKMFE TEMKIAAMHV KRKQLHQLQP SHVSPKKKKH SFEGVKLLSL
510 520 530 540 550
NDSSIDLSVD SDNSMSVPSP TNATRTSPLN SSGLSQGNSP AAPVSFSVTN
560 570 580 590 600
VQATDVMVPQ NNSTENLGGS LNESIPESAT HPGFSSTPKP LVTRVVSSMR
610 620 630 640 650
LVNQLQKPVS NTITKMPSPV AGVKRTSSPS NEDSPKKNKT EEDENDSSIS
660 670 680 690 700
ADVDDQNKLE TEELKEVHSE EKSSSPVPGS LPFSQQSSTD LSDISVVPAT
710
PIPVIKNSIK LRLNR
Length:715
Mass (Da):80,188
Last modified:October 1, 1996 - v1
Checksum:i355D74F250AD14A9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19973 mRNA. Translation: AAC59745.1.
UniGeneiXl.57169.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19973 mRNA. Translation: AAC59745.1.
UniGeneiXl.57169.

3D structure databases

ProteinModelPortaliP51004.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP51004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG053502.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR10682. PTHR10682. 1 hit.
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPAPO1_XENLA
AccessioniPrimary (citable) accession number: P51004
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 5, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.