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P51004

- PAPO1_XENLA

UniProt

P51004 - PAPO1_XENLA

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Protein

Poly(A) polymerase alpha-A

Gene

papola-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor (CPSF).

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions. Also active with manganese.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei91 – 911ATPBy similarity
Metal bindingi95 – 951Magnesium 1; catalyticBy similarity
Metal bindingi95 – 951Magnesium 2; catalyticBy similarity
Metal bindingi97 – 971Magnesium 1; catalyticBy similarity
Metal bindingi97 – 971Magnesium 2; catalyticBy similarity
Sitei135 – 1351Interaction with RNABy similarity
Sitei140 – 1401Interaction with RNABy similarity
Metal bindingi149 – 1491Magnesium 2; catalyticBy similarity
Binding sitei149 – 1491ATPBy similarity
Binding sitei210 – 2101ATPBy similarity
Binding sitei219 – 2191ATPBy similarity
Sitei310 – 3101Interaction with RNABy similarity
Sitei381 – 3811Interaction with RNABy similarity
Sitei506 – 5061Interaction with RNABy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi82 – 843ATPBy similarity
Nucleotide bindingi95 – 973ATPBy similarity
Nucleotide bindingi228 – 2292ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: UniProtKB-EC
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA processing Source: UniProtKB-KW
  2. RNA polyadenylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase alpha-A (EC:2.7.7.19)
Short name:
PAP-alpha-A
Alternative name(s):
Polynucleotide adenylyltransferase alpha-A
Gene namesi
Name:papola-a
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 715›715Poly(A) polymerase alpha-APRO_0000051614Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP51004.
SMRiP51004. Positions 4-480.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi472 – 48918Nuclear localization signal 1By similarityAdd
BLAST
Motifi624 – 63916Nuclear localization signal 2By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the poly(A) polymerase family.Curated

Phylogenomic databases

HOVERGENiHBG053502.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Fragment.

P51004-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
PKTFGITSPI SLAAAKDTDC TLMQKLIETL KPYGVFEEEE QLQHRILGKL
60 70 80 90 100
NNLVKEWIRE ISELKHLPQS VIENVGGKIF TFGPYRLGVH TKGADIDALC
110 120 130 140 150
VAPRHVDRSD FFSSFYDKLK QQEEVKDLRS VEEAFVPVIK LCFDGIEIDI
160 170 180 190 200
LFARLALQTI PEDLDLRDDS LLKNLDIRCI RSLNGCRVTD EILHLVPNID
210 220 230 240 250
SFRLTLRAIK LWAKRHNIYS NILGFLGGVS WAMLVARTCQ LYPNAIASTL
260 270 280 290 300
VHKFFLVFSK WEWPNPVLLK QPEECNLNLP VWDPRVNPSD RYHLMPIITP
310 320 330 340 350
AYPQQNSTYN VSVSTRAVMI EEFKQGLAIT DEILLGKAEW SKLFDAPNFF
360 370 380 390 400
QKYKHYILLL ASAPTEKQRL EWVGLVESKI RILVGSLEKN EFITLAHVNP
410 420 430 440 450
RSFPAPSENM EKEEFRTMWV IGLVFKKMEN SENLSVDLTY DIQSFTDTVD
460 470 480 490 500
RQAINSKMFE TEMKIAAMHV KRKQLHQLQP SHVSPKKKKH SFEGVKLLSL
510 520 530 540 550
NDSSIDLSVD SDNSMSVPSP TNATRTSPLN SSGLSQGNSP AAPVSFSVTN
560 570 580 590 600
VQATDVMVPQ NNSTENLGGS LNESIPESAT HPGFSSTPKP LVTRVVSSMR
610 620 630 640 650
LVNQLQKPVS NTITKMPSPV AGVKRTSSPS NEDSPKKNKT EEDENDSSIS
660 670 680 690 700
ADVDDQNKLE TEELKEVHSE EKSSSPVPGS LPFSQQSSTD LSDISVVPAT
710
PIPVIKNSIK LRLNR
Length:715
Mass (Da):80,188
Last modified:October 1, 1996 - v1
Checksum:i355D74F250AD14A9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19973 mRNA. Translation: AAC59745.1.
UniGeneiXl.57169.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19973 mRNA. Translation: AAC59745.1 .
UniGenei Xl.57169.

3D structure databases

ProteinModelPortali P51004.
SMRi P51004. Positions 4-480.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG053502.

Family and domain databases

Gene3Di 3.30.70.590. 1 hit.
InterProi IPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view ]
Pfami PF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view ]
PIRSFi PIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMi SSF55003. SSF55003. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Poly(A) polymerases in the nucleus and cytoplasm of frog oocytes: dynamic changes during oocyte maturation and early development."
    Ballantyne S., Bilger A., Astrom J., Virtanen A., Wickens M.
    RNA 1:64-78(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiPAPO1_XENLA
AccessioniPrimary (citable) accession number: P51004
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3