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Protein

AMP deaminase

Gene

ada1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

AMP deaminase plays a critical role in energy metabolism.

Catalytic activityi

AMP + H2O = IMP + NH3.1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: IMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from AMP.
Proteins known to be involved in this subpathway in this organism are:
  1. AMP deaminase (ada1)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from AMP, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi319Zinc; catalyticBy similarity1
Metal bindingi321Zinc; catalyticBy similarity1
Binding sitei321SubstrateBy similarity1
Metal bindingi587Zinc; catalyticBy similarity1
Binding sitei590SubstrateBy similarity1
Active sitei609Proton acceptorPROSITE-ProRule annotation1
Metal bindingi664Zinc; catalyticBy similarity1

GO - Molecular functioni

  • AMP deaminase activity Source: PomBase
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SPO-6798695. Neutrophil degranulation.
R-SPO-74217. Purine salvage.
UniPathwayiUPA00591; UER00663.

Names & Taxonomyi

Protein namesi
Recommended name:
AMP deaminase (EC:3.5.4.6)
Alternative name(s):
Myoadenylate deaminase
Gene namesi
Name:ada1
ORF Names:SPBC106.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC106.04.
PomBaseiSPBC106.04. ada1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001944131 – 831AMP deaminaseAdd BLAST831

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei79Phosphoserine1 Publication1
Modified residuei84Phosphoserine1 Publication1
Modified residuei758Phosphoserine1 Publication1
Modified residuei776Phosphoserine1 Publication1
Modified residuei780Phosphoserine1 Publication1
Modified residuei782Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP50998.
PRIDEiP50998.

PTM databases

iPTMnetiP50998.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi276642. 73 interactors.
MINTiMINT-4690806.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni390 – 395Substrate bindingBy similarity6
Regioni665 – 668Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000092200.
InParanoidiP50998.
KOiK01490.
OMAiYENDSAY.
OrthoDBiEOG092C0YBC.

Family and domain databases

CDDicd01319. AMPD. 1 hit.
InterProiIPR006650. A/AMP_deam_AS.
IPR001365. A/AMP_deaminase_dom.
IPR006329. AMPD.
IPR032466. Metal_Hydrolase.
[Graphical view]
PANTHERiPTHR11359. PTHR11359. 1 hit.
PfamiPF00962. A_deaminase. 1 hit.
[Graphical view]
PIRSFiPIRSF001251. AMP_deaminase_met. 1 hit.
SUPFAMiSSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01429. AMP_deaminase. 1 hit.
PROSITEiPS00485. A_DEAMINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50998-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNMEQEDDQV PAVAAETVPL KRYVTNPGAN RDEEVAAAPS SQDTPYFDYA
60 70 80 90 100
YERSLRHQDA KFLAMNGTQN GRDGLPSKSP RRPSVSASTV RNSDDVNHSK
110 120 130 140 150
AGPGSGKLLN DTLQSKISSI HMPHVQQGDN AVVSSVGGPE TDPGNMETTD
160 170 180 190 200
PLFSDELAEI YLSIHKCMDM RHKYIRVSLQ GELDNPIDDD SWIIYPDCKE
210 220 230 240 250
GEDDTGLFNF ADCKIPGIEN EMEYHMDHQG IFQVYENDSA YIAGTPSFHI
260 270 280 290 300
PTIRDYYIDL EFLLSASSDG PSKSFSFRRL QYLEGRWNMY MLLNEYQELA
310 320 330 340 350
DTKKVPHRDF YNVRKVDTHV HHSALANQKH LLRFIKAKLR KCPNEKVIWR
360 370 380 390 400
DGKFLTLQEV FDSLKLTSYD LSIDTLDMHA HTDTFHRFDK FNLKYNPIGE
410 420 430 440 450
SRLRTIFLKT DNDINGRYLA ELTKEVFTDL RTQKYQMAEY RISIYGRNRE
460 470 480 490 500
EWDKLAAWII DNELFSPNVR WLIQVPRLYD VYKKSGIVET FEEVVRNVFE
510 520 530 540 550
PLFEVTKDPR THPKLHVFLQ RVIGFDSVDD ESKPERRTFR KFPYPKHWDI
560 570 580 590 600
NLNPPYSYWL YYMYANMTSL NSWRKIRGFN TFVLRPHCGE AGDTDHLASA
610 620 630 640 650
FLLSHGINHG ILLRKVPFLQ YLWYLDQIPI AMSPLSNNAL FLAYDKNPFL
660 670 680 690 700
TYFKRGLNVS LSTDDPLQFA FTREPLIEEY AVAAQIYKLS AVDMCELARN
710 720 730 740 750
SVLQSGFERQ LKERWLGVDF QDIDRTNVPI IRLAYRALTL TQEIALVNKH
760 770 780 790 800
VQPSKHPSNH DLEELIHKYD AMTGTSDPLS ASPRTNDATI SSRLSLHDGH
810 820 830
DHGAFFPGLS VISERRRRKD SMASSSQDLK D
Length:831
Mass (Da):95,887
Last modified:July 11, 2012 - v3
Checksum:iF027B33C7AD0C63D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti468 – 505NVRWL…PLFEV → TFVGLFKYLVCMMCIRSPVL LRLLKRSSEMSLNHCSKF in CAA62797 (Ref. 1) CuratedAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91498 Genomic DNA. Translation: CAA62797.1. Sequence problems.
CU329671 Genomic DNA. Translation: CAB53720.2.
PIRiT39261.
RefSeqiNP_595153.2. NM_001021062.2.

Genome annotation databases

EnsemblFungiiSPBC106.04.1; SPBC106.04.1:pep; SPBC106.04.
GeneIDi2540105.
KEGGispo:SPBC106.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91498 Genomic DNA. Translation: CAA62797.1. Sequence problems.
CU329671 Genomic DNA. Translation: CAB53720.2.
PIRiT39261.
RefSeqiNP_595153.2. NM_001021062.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276642. 73 interactors.
MINTiMINT-4690806.

PTM databases

iPTMnetiP50998.

Proteomic databases

MaxQBiP50998.
PRIDEiP50998.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC106.04.1; SPBC106.04.1:pep; SPBC106.04.
GeneIDi2540105.
KEGGispo:SPBC106.04.

Organism-specific databases

EuPathDBiFungiDB:SPBC106.04.
PomBaseiSPBC106.04. ada1.

Phylogenomic databases

HOGENOMiHOG000092200.
InParanoidiP50998.
KOiK01490.
OMAiYENDSAY.
OrthoDBiEOG092C0YBC.

Enzyme and pathway databases

UniPathwayiUPA00591; UER00663.
ReactomeiR-SPO-6798695. Neutrophil degranulation.
R-SPO-74217. Purine salvage.

Miscellaneous databases

PROiP50998.

Family and domain databases

CDDicd01319. AMPD. 1 hit.
InterProiIPR006650. A/AMP_deam_AS.
IPR001365. A/AMP_deaminase_dom.
IPR006329. AMPD.
IPR032466. Metal_Hydrolase.
[Graphical view]
PANTHERiPTHR11359. PTHR11359. 1 hit.
PfamiPF00962. A_deaminase. 1 hit.
[Graphical view]
PIRSFiPIRSF001251. AMP_deaminase_met. 1 hit.
SUPFAMiSSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01429. AMP_deaminase. 1 hit.
PROSITEiPS00485. A_DEAMINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPD_SCHPO
AccessioniPrimary (citable) accession number: P50998
Secondary accession number(s): Q9URV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 11, 2012
Last modified: November 30, 2016
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.