T-complex protein 1 subunit theta - Homo sapiens (Human)

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P50990 (TCPQ_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 139. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
T-complex protein 1 subunit theta
Short name=TCP-1-theta

Alternative name(s):
CCT-theta
Renal carcinoma antigen NY-REN-15

Gene names

Name:	CCT8
Synonyms:	C21orf112, CCTQ, KIAA0002

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Ref.15
Subunit structure	Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Component of the BBS/CCT complex composed at least of MKKS, BBS10, BBS12, TCP1, CCT2, CCT3, CCT4, CCT5 AND CCT8. Ref.9 Ref.15
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome Ref.15.
Sequence similarities	Belongs to the TCP-1 chaperonin family.
Sequence caution	The sequence BAA02792.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' posttranslational protein folding Traceable author statement. Source: Reactome binding of sperm to zona pellucida Inferred from electronic annotation. Source: Ensembl
Cellular_component	aggresome Inferred from direct assay. Source: HPA cell body Inferred from electronic annotation. Source: Ensembl centrosome Inferred from direct assay Ref.15. Source: MGI chaperonin-containing T-complex Inferred from electronic annotation. Source: Ensembl cytosol Inferred from direct assay PubMed 16780588. Source: UniProtKB intermediate filament cytoskeleton Inferred from direct assay. Source: HPA microtubule Inferred from direct assay PubMed 21525035. Source: UniProtKB zona pellucida receptor complex Inferred from electronic annotation. Source: Ensembl
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled Traceable author statement Ref.7. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.6

Chain

2 – 548

547

T-complex protein 1 subunit theta

PRO_0000128373

Amino acid modifications

Modified residue

N-acetylalanine Ref.6

Modified residue

Phosphoserine Ref.12 Ref.13

Modified residue

Phosphotyrosine Ref.13

Modified residue

318

N6-acetyllysine Ref.14

Modified residue

400

N6-acetyllysine Ref.14

Modified residue

466

N6-acetyllysine Ref.14

Modified residue

505

Phosphotyrosine Ref.10

Cross-link

225

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.11

Cross-link

235

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.11

Natural variations

Natural variant

H → Q.
Corresponds to variant rs16983693 [ dbSNP | Ensembl ].

VAR_052270

Natural variant

409

V → I.
Corresponds to variant rs8129954 [ dbSNP | Ensembl ].

VAR_052271

Experimental info

Sequence conflict

N → K Ref.1

Sequence conflict

N → K Ref.2

Sequence conflict

391

A → V in BAA02792. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P50990 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 566A6622BC2D15E9
Show »

FASTA

548

59,621

        10         20         30         40         50         60 
MALHVPKAPG FAQMLKEGAK HFSGLEEAVY RNIQACKELA QTTRTAYGPN GMNKMVINHL 

        70         80         90        100        110        120 
EKLFVTNDAA TILRELEVQH PAAKMIVMAS HMQEQEVGDG TNFVLVFAGA LLELAEELLR 

       130        140        150        160        170        180 
IGLSVSEVIE GYEIACRKAH EILPNLVCCS AKNLRDIDEV SSLLRTSIMS KQYGNEVFLA 

       190        200        210        220        230        240 
KLIAQACVSI FPDSGHFNVD NIRVCKILGS GISSSSVLHG MVFKKETEGD VTSVKDAKIA 

       250        260        270        280        290        300 
VYSCPFDGMI TETKGTVLIK TAEELMNFSK GEENLMDAQV KAIADTGANV VVTGGKVADM 

       310        320        330        340        350        360 
ALHYANKYNI MLVRLNSKWD LRRLCKTVGA TALPRLTPPV LEEMGHCDSV YLSEVGDTQV 

       370        380        390        400        410        420 
VVFKHEKEDG AISTIVLRGS TDNLMDDIER AVDDGVNTFK VLTRDKRLVP GGGATEIELA 

       430        440        450        460        470        480 
KQITSYGETC PGLEQYAIKK FAEAFEAIPR ALAENSGVKA NEVISKLYAV HQEGNKNVGL 

       490        500        510        520        530        540 
DIEAEVPAVK DMLEAGILDT YLGKYWAIKL ATNAAVTVLR VDQIIMAKPA GGPKPPSGKK 


DWDDDQND

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1." Nomura N., Miyajima N., Sazuka T., Tanaka A., Kawarabayasi Y., Sato S., Nagase T., Seki N., Ishikawa K., Tabata S. DNA Res. 1:27-35(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow.
[2]	"Nucleotide sequence surrounding the locus marker D21S246 on human chromosome 21." Yamazaki M., Ono A., Watanabe K., Sasaki K., Tashiro H., Nomura T. DNA Res. 2:187-189(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Taudien S., Dagand E., Delabar J., Nordsiek G., Drescher B., Weber J., Schattevoy R., Menzel U., Yaspo M.-L., Rosenthal A. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The DNA sequence of human chromosome 21." Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. Yaspo M.-L. Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Chondrosarcoma and Liver.
[6]	Bienvenut W.V. Submitted (OCT-2004) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-16; 55-74; 156-165; 172-181; 283-296; 408-421; 441-450 AND 510-520, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: B-cell lymphoma.
[7]	"The eighth Cct gene, Cctq, encoding the theta subunit of the cytosolic chaperonin containing TCP-1." Kubota H., Hynes G., Willison K. Gene 154:231-236(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-65.
[8]	"Antigens recognized by autologous antibody in patients with renal-cell carcinoma." Scanlan M.J., Gordan J.D., Williamson B., Stockert E., Bander N.H., Jongeneel C.V., Gure A.O., Jaeger D., Jaeger E., Knuth A., Chen Y.-T., Old L.J. Int. J. Cancer 83:456-464(1999) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS A RENAL CANCER ANTIGEN. Tissue: Renal cell carcinoma.
[9]	"A product of the human gene adjacent to parkin is a component of Lewy bodies and suppresses Pael receptor-induced cell death." Imai Y., Soda M., Murakami T., Shoji M., Abe K., Takahashi R. J. Biol. Chem. 278:51901-51910(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PACRG.
[10]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-505, MASS SPECTROMETRY.
[11]	"Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry." Denis N.J., Vasilescu J., Lambert J.-P., Smith J.C., Figeys D. Proteomics 7:868-874(2007) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-225 AND LYS-235, MASS SPECTROMETRY. Tissue: Mammary cancer.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23 AND TYR-30, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[14]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-318; LYS-400 AND LYS-466, MASS SPECTROMETRY.
[15]	"BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly." Seo S., Baye L.M., Schulz N.P., Beck J.S., Zhang Q., Slusarski D.C., Sheffield V.C. Proc. Natl. Acad. Sci. U.S.A. 107:1488-1493(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN BBS/CCT COMPLEX.
[16]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13627 mRNA. Translation: BAA02792.2. Different initiation. D42052 Genomic DNA. Translation: BAA07652.1. Sequence problems. AF129075 Genomic DNA. No translation available. AL163249 Genomic DNA. Translation: CAB90433.1. BC072001 mRNA. Translation: AAH72001.1. BC095470 mRNA. Translation: AAH95470.1. Z37163 mRNA. Translation: CAA85520.1.
PIR	PC4021.
RefSeq	NP_001269836.1. NM_001282907.1. NP_001269838.1. NM_001282909.1. NP_006576.2. NM_006585.3.
UniGene	Hs.125113.
3D structure databases
ProteinModelPortal	P50990.
SMR	P50990. Positions 13-528.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	115933. 92 interactions.
DIP	DIP-38124N.
IntAct	P50990. 39 interactions.
MINT	MINT-1152593.
STRING	9606.ENSP00000286788.
PTM databases
PhosphoSite	P50990.
Polymorphism databases
DMDM	9988062.
2D gel databases
OGP	P50990.
REPRODUCTION-2DPAGE	IPI00784090. P50990.
Proteomic databases
PaxDb	P50990.
PRIDE	P50990.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000286788; ENSP00000286788; ENSG00000156261.
GeneID	10694.
KEGG	hsa:10694.
UCSC	uc002yna.3. human.
Organism-specific databases
CTD	10694.
GeneCards	GC21M030428.
HGNC	HGNC:1623. CCT8.
HPA	HPA018520. HPA021051. HPA029426.
neXtProt	NX_P50990.
PharmGKB	PA26186.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0459.
HOGENOM	HOG000226734.
HOVERGEN	HBG103107.
InParanoid	P50990.
KO	K09500.
OMA	KDWDDDQ.
OrthoDB	EOG7JQBN8.
TreeFam	TF105699.
Enzyme and pathway databases
Reactome	REACT_17015. Metabolism of proteins.
Gene expression databases
ArrayExpress	P50990.
Bgee	P50990.
CleanEx	HS_CCT8.
Genevestigator	P50990.
Family and domain databases
Gene3D	1.10.560.10. 2 hits. 3.30.260.10. 2 hits. 3.50.7.10. 1 hit.
InterPro	IPR012721. Chap_CCT_theta. IPR017998. Chaperone_TCP-1. IPR002194. Chaperonin_TCP-1_CS. IPR002423. Cpn60/TCP-1. IPR027409. GroEL-like_apical_dom. IPR027413. GROEL-like_equatorial. IPR027410. TCP-1-like_intermed. [Graphical view]
PANTHER	PTHR11353. PTHR11353. 1 hit.
Pfam	PF00118. Cpn60_TCP1. 1 hit. [Graphical view]
PRINTS	PR00304. TCOMPLEXTCP1.
SUPFAM	SSF48592. SSF48592. 2 hits. SSF52029. SSF52029. 1 hit.
TIGRFAMs	TIGR02346. chap_CCT_theta. 1 hit.
PROSITE	PS00750. TCP1_1. 1 hit. PS00751. TCP1_2. 1 hit. PS00995. TCP1_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CCT8. human.
GeneWiki	CCT8.
GenomeRNAi	10694.
NextBio	40649.
PRO	P50990.

Entry information

Entry name

TCPQ_HUMAN

Accession

Primary (citable) accession number: P50990
Secondary accession number(s): A6NN54, Q4VBP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	February 19, 2014
This is version 139 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 21

Human chromosome 21: entries, gene names and cross-references to MIM

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents