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UniProtKB - P50990 (TCPQ_HUMAN)

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Protein

T-complex protein 1 subunit theta

Gene

CCT8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.1 Publication

Caution

GO - Molecular functioni

  • ATPase activity, coupled Source: ProtInc
  • ATP binding Source: UniProtKB-KW
  • cadherin binding Source: BHF-UCL
  • protein binding involved in protein folding Source: GO_Central
  • unfolded protein binding Source: GO_Central
View the complete GO annotation on QuickGO ...

GO - Biological processi

  • 'de novo' protein folding Source: GO_Central
  • binding of sperm to zona pellucida Source: Ensembl
  • chaperone-mediated protein folding Source: GO_Central
  • neutrophil degranulation Source: Reactome
  • pore complex assembly Source: Ensembl
  • positive regulation of establishment of protein localization to telomere Source: BHF-UCL
  • positive regulation of protein localization to Cajal body Source: BHF-UCL
  • positive regulation of telomerase RNA localization to Cajal body Source: BHF-UCL
  • positive regulation of telomere maintenance via telomerase Source: BHF-UCL
  • protein folding Source: Reactome
  • protein stabilization Source: BHF-UCL
  • toxin transport Source: Ensembl
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionChaperone
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-389957 Prefoldin mediated transfer of substrate to CCT/TriC
R-HSA-389960 Formation of tubulin folding intermediates by CCT/TriC
R-HSA-390450 Folding of actin by CCT/TriC
R-HSA-390471 Association of TriC/CCT with target proteins during biosynthesis
R-HSA-5620922 BBSome-mediated cargo-targeting to cilium
R-HSA-6798695 Neutrophil degranulation
R-HSA-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Names & Taxonomyi

Protein namesi
Recommended name:
T-complex protein 1 subunit theta
Short name:
TCP-1-theta
Alternative name(s):
CCT-theta
Chaperonin containing T-complex polypeptide 1 subunit 8
Renal carcinoma antigen NY-REN-15
Gene namesi
Name:CCT8
Synonyms:C21orf112, CCTQ, KIAA0002
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 21

Organism-specific databases

EuPathDBiHostDB:ENSG00000156261.12
HGNCiHGNC:1623 CCT8
MIMi617786 gene
neXtProtiNX_P50990

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

DisGeNETi10694
OpenTargetsiENSG00000156261
PharmGKBiPA26186

Polymorphism and mutation databases

BioMutaiCCT8
DMDMi9988062

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001283732 – 548T-complex protein 1 subunit thetaAdd BLAST547

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei23PhosphoserineCombined sources1
Modified residuei30PhosphotyrosineCombined sources1
Modified residuei162PhosphoserineCombined sources1
Modified residuei213PhosphoserineCombined sources1
Cross-linki224Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki254Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki260Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei269PhosphoserineCombined sources1
Modified residuei317PhosphoserineCombined sources1
Modified residuei318N6-acetyllysineCombined sources1
Modified residuei400N6-acetyllysineCombined sources1
Cross-linki459Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources
Modified residuei466N6-acetyllysineCombined sources1
Modified residuei505PhosphotyrosineCombined sources1
Cross-linki534Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei537PhosphoserineCombined sources1
Cross-linki539Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP50990
MaxQBiP50990
PaxDbiP50990
PeptideAtlasiP50990
PRIDEiP50990
TopDownProteomicsiP50990-1 [P50990-1]

2D gel databases

OGPiP50990
REPRODUCTION-2DPAGEiIPI00784090
P50990

PTM databases

iPTMnetiP50990
PhosphoSitePlusiP50990
SwissPalmiP50990

Expressioni

Gene expression databases

BgeeiENSG00000156261
CleanExiHS_CCT8
ExpressionAtlasiP50990 baseline and differential
GenevisibleiP50990 HS

Organism-specific databases

HPAiHPA018520
HPA021051
HPA029426

Interactioni

Subunit structurei

Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Component of the BBS/CCT complex composed at least of MKKS, BBS10, BBS12, TCP1, CCT2, CCT3, CCT4, CCT5 AND CCT8. Interacts with DNAAF4 (By similarity).By similarity

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi115933199 interactors.
CORUMiP50990
DIPiDIP-38124N
IntActiP50990 120 interactors.
MINTiP50990
STRINGi9606.ENSP00000286788

Structurei

3D structure databases

ProteinModelPortaliP50990
SMRiP50990
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TCP-1 chaperonin family.Curated

Phylogenomic databases

eggNOGiKOG0362 Eukaryota
ENOG410XPXR LUCA
GeneTreeiENSGT00550000074783
HOGENOMiHOG000226734
HOVERGENiHBG103107
InParanoidiP50990
KOiK09500
OMAiWGLKYAV
OrthoDBiEOG091G05WZ
PhylomeDBiP50990
TreeFamiTF105699

Family and domain databases

CDDicd03341 TCP1_theta, 1 hit
Gene3Di1.10.560.102 hits
3.30.260.102 hits
3.50.7.101 hit
InterProiView protein in InterPro
IPR012721 Chap_CCT_theta
IPR017998 Chaperone_TCP-1
IPR002194 Chaperonin_TCP-1_CS
IPR002423 Cpn60/TCP-1
IPR027409 GroEL-like_apical_dom_sf
IPR027413 GROEL-like_equatorial_sf
IPR027410 TCP-1-like_intermed_sf
PANTHERiPTHR11353 PTHR11353, 1 hit
PfamiView protein in Pfam
PF00118 Cpn60_TCP1, 1 hit
PRINTSiPR00304 TCOMPLEXTCP1
SUPFAMiSSF48592 SSF48592, 2 hits
SSF52029 SSF52029, 1 hit
SSF54849 SSF54849, 2 hits
TIGRFAMsiTIGR02346 chap_CCT_theta, 1 hit
PROSITEiView protein in PROSITE
PS00750 TCP1_1, 1 hit
PS00751 TCP1_2, 1 hit
PS00995 TCP1_3, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P50990-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALHVPKAPG FAQMLKEGAK HFSGLEEAVY RNIQACKELA QTTRTAYGPN 
        60         70         80         90        100
GMNKMVINHL EKLFVTNDAA TILRELEVQH PAAKMIVMAS HMQEQEVGDG 
       110        120        130        140        150
TNFVLVFAGA LLELAEELLR IGLSVSEVIE GYEIACRKAH EILPNLVCCS 
       160        170        180        190        200
AKNLRDIDEV SSLLRTSIMS KQYGNEVFLA KLIAQACVSI FPDSGHFNVD 
       210        220        230        240        250
NIRVCKILGS GISSSSVLHG MVFKKETEGD VTSVKDAKIA VYSCPFDGMI 
       260        270        280        290        300
TETKGTVLIK TAEELMNFSK GEENLMDAQV KAIADTGANV VVTGGKVADM 
       310        320        330        340        350
ALHYANKYNI MLVRLNSKWD LRRLCKTVGA TALPRLTPPV LEEMGHCDSV 
       360        370        380        390        400
YLSEVGDTQV VVFKHEKEDG AISTIVLRGS TDNLMDDIER AVDDGVNTFK 
       410        420        430        440        450
VLTRDKRLVP GGGATEIELA KQITSYGETC PGLEQYAIKK FAEAFEAIPR 
       460        470        480        490        500
ALAENSGVKA NEVISKLYAV HQEGNKNVGL DIEAEVPAVK DMLEAGILDT 
       510        520        530        540 
YLGKYWAIKL ATNAAVTVLR VDQIIMAKPA GGPKPPSGKK DWDDDQND
Length:548
Mass (Da):59,621
Last modified:January 23, 2007 - v4
Checksum:i566A6622BC2D15E9
GO
Isoform 2 (identifier: P50990-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MALHVPKAPGFAQMLKEGAK → M

Note: No experimental confirmation available.
Show »
Length:529
Mass (Da):57,645
Checksum:i7468208B8A3B84E2
GO
Isoform 3 (identifier: P50990-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: MALHVPKAPG...AATILRELEV → MDQMV

Note: No experimental confirmation available.
Show »
Length:475
Mass (Da):51,586
Checksum:i2D97E8F445125063
GO

Sequence cautioni

The sequence BAA02792 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50N → K (PubMed:7584026).Curated1
Sequence conflicti50N → K (PubMed:8590283).Curated1
Sequence conflicti391A → V in BAA02792 (PubMed:7584026).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0522704H → Q. Corresponds to variant dbSNP:rs16983693Ensembl.1
Natural variantiVAR_052271409V → I. Corresponds to variant dbSNP:rs8129954Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0546911 – 78MALHV…RELEV → MDQMV in isoform 3. 1 PublicationAdd BLAST78
Alternative sequenceiVSP_0546921 – 20MALHV…KEGAK → M in isoform 2. 1 PublicationAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13627 mRNA Translation: BAA02792.2 Different initiation.
D42052 Genomic DNA Translation: BAA07652.1 Sequence problems.
AF129075 Genomic DNA No translation available.
AK293705 mRNA Translation: BAG57138.1
AK298801 mRNA Translation: BAG60936.1
AL163249 Genomic DNA Translation: CAB90433.1
BC072001 mRNA Translation: AAH72001.1
BC095470 mRNA Translation: AAH95470.1
Z37163 mRNA Translation: CAA85520.1
CCDSiCCDS33528.1 [P50990-1]
CCDS68180.1 [P50990-3]
CCDS68181.1 [P50990-2]
PIRiPC4021
RefSeqiNP_001269836.1, NM_001282907.1 [P50990-2]
NP_001269838.1, NM_001282909.1 [P50990-3]
NP_006576.2, NM_006585.3 [P50990-1]
UniGeneiHs.125113

Genome annotation databases

EnsembliENST00000286788; ENSP00000286788; ENSG00000156261 [P50990-1]
ENST00000540844; ENSP00000442730; ENSG00000156261 [P50990-3]
ENST00000626972; ENSP00000486921; ENSG00000156261 [P50990-2]
GeneIDi10694
KEGGihsa:10694
UCSCiuc002ynb.5 human [P50990-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTCPQ_HUMAN
AccessioniPrimary (citable) accession number: P50990
Secondary accession number(s): A6NN54
, B4DEM7, B4DQH4, Q4VBP8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 183 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome