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P50984

- CA1A_CONPE

UniProt

P50984 - CA1A_CONPE

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Protein

Alpha-conotoxin PnIA

Gene
N/A
Organism
Conus pennaceus (Feathered cone) (Conus episcopus)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks mammalian nAChRs (alpha-3/beta-2 > alpha-7 > alpha-3/beta-4).2 Publications

Keywords - Molecular functioni

Acetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-conotoxin PnIA
Short name:
Alpha-PnIA
OrganismiConus pennaceus (Feathered cone) (Conus episcopus)
Taxonomic identifieri37335 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Organism-specific databases

ConoServeri75. Pni1.
51. PnIA.

Subcellular locationi

GO - Cellular componenti

  1. other organism postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi10 – 101A → L: Blocks nAChr alpha-7 subunits with higher potency than PnIA and PnIB. Blocks nAChR alpha-3/beta-2 subunits with a potency range between the potency of PnIA and PnIB. 1 Publication
Mutagenesisi11 – 111N → S: Blocks nAChR alpha-7 subunits with lower potency than PnIA and PnIB. Blocks nAChR alpha-3/beta-2 subunits with a potency range between the potency of PnIA and PnIB. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 1616Alpha-conotoxin PnIAPRO_0000044462Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi2 ↔ 82 Publications
Disulfide bondi3 ↔ 162 Publications
Modified residuei15 – 151Sulfotyrosine1 Publication
Modified residuei16 – 161Cysteine amide1 Publication

Keywords - PTMi

Amidation, Disulfide bond, Sulfation

Expressioni

Tissue specificityi

Expressed by the venom duct.

Interactioni

Protein-protein interaction databases

DIPiDIP-60493N.

Structurei

Secondary structure

1
16
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 43
Helixi6 – 116
Turni13 – 153

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PENX-ray1.10A1-16[»]
2BR8X-ray2.40F/G/H/I/J1-16[»]

Miscellaneous databases

EvolutionaryTraceiP50984.

Family & Domainsi

Domaini

The cysteine framework is I (CC-C-C). Alpha4/7 pattern.

Sequence similaritiesi

Belongs to the conotoxin A superfamily.

Family and domain databases

InterProiIPR018072. Conotoxin_a-typ_CS.
[Graphical view]
PROSITEiPS60014. ALPHA_CONOTOXIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50984-1 [UniParc]FASTAAdd to Basket

« Hide

GCCSLPPCAA NNPDYC                                        16
Length:16
Mass (Da):1,628
Last modified:October 1, 1996 - v1
Checksum:i05310FF95EC99005
GO

Sequence databases

PIRiA54877.

Cross-referencesi

Sequence databases

PIRi A54877.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1PEN X-ray 1.10 A 1-16 [» ]
2BR8 X-ray 2.40 F/G/H/I/J 1-16 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60493N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

ConoServeri 75. Pni1.
51. PnIA.

Miscellaneous databases

EvolutionaryTracei P50984.

Family and domain databases

InterProi IPR018072. Conotoxin_a-typ_CS.
[Graphical view ]
PROSITEi PS60014. ALPHA_CONOTOXIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "New mollusc-specific alpha-conotoxins block Aplysia neuronal acetylcholine receptors."
    Fainzilber M., Hasson A., Oren R., Burlingame A.L., Gordon D., Spira M.E., Zlotkin E.
    Biochemistry 33:9523-9529(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION.
    Tissue: Venom.
  2. "Identification of tyrosine sulfation in Conus pennaceus conotoxins alpha-PnIA and alpha-PnIB: further investigation of labile sulfo- and phosphopeptides by electrospray, matrix-assisted laser desorption/ionization (MALDI) and atmospheric pressure MALDI mass spectrometry."
    Wolfender J.L., Chu F., Ball H., Wolfender F., Fainzilber M., Baldwin M.A., Burlingame A.L.
    J. Mass Spectrom. 34:447-454(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SULFATION AT TYR-15.
  3. "Single-residue alteration in alpha-conotoxin PnIA switches its nAChR subtype selectivity."
    Luo S., Nguyen T.A., Cartier G.E., Olivera B.M., Yoshikami D., McIntosh J.M.
    Biochemistry 38:14542-14548(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SYNTHESIS, MUTAGENESIS OF ALA-10 AND ASN-11.
  4. "The 1.1 A crystal structure of the neuronal acetylcholine receptor antagonist, alpha-conotoxin PnIA from Conus pennaceus."
    Hu S.-H., Gehrmann J., Guddat L.W., Alewood P.F., Craik D.J., Martin J.L.
    Structure 4:417-423(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS), DISULFIDE BONDS.

Entry informationi

Entry nameiCA1A_CONPE
AccessioniPrimary (citable) accession number: P50984
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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