Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P50984 (CA1A_CONPE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-conotoxin PnIA

Short name=Alpha-PnIA
OrganismConus pennaceus (Feathered cone) (Conus episcopus)
Taxonomic identifier37335 [NCBI]
Taxonomic lineageEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Protein attributes

Sequence length16 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks mammalian nAChRs (alpha-3/beta-2 > alpha-7 > alpha-3/beta-4). Ref.1 Ref.3

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom duct.

Domain

The cysteine framework is I (CC-C-C).

Sequence similarities

Belongs to the conotoxin A superfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Peptide1 – 1616Alpha-conotoxin PnIA
PRO_0000044462

Amino acid modifications

Modified residue151Sulfotyrosine Ref.2
Modified residue161Cysteine amide Ref.1
Disulfide bond2 ↔ 8 Ref.1 Ref.4
Disulfide bond3 ↔ 16 Ref.1 Ref.4

Experimental info

Mutagenesis101A → L: Blocks nAChr alpha-7 subunits with higher potency than PnIA and PnIB. Blocks nAChR alpha-3/beta-2 subunits with a potency range between the potency of PnIA and PnIB. Ref.3
Mutagenesis111N → S: Blocks nAChR alpha-7 subunits with lower potency than PnIA and PnIB. Blocks nAChR alpha-3/beta-2 subunits with a potency range between the potency of PnIA and PnIB. Ref.3

Secondary structure

....... 16
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P50984 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 05310FF95EC99005

FASTA161,628
        10 
GCCSLPPCAA NNPDYC 

« Hide

References

[1]"New mollusc-specific alpha-conotoxins block Aplysia neuronal acetylcholine receptors."
Fainzilber M., Hasson A., Oren R., Burlingame A.L., Gordon D., Spira M.E., Zlotkin E.
Biochemistry 33:9523-9529(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, FUNCTION.
Tissue: Venom.
[2]"Identification of tyrosine sulfation in Conus pennaceus conotoxins alpha-PnIA and alpha-PnIB: further investigation of labile sulfo- and phosphopeptides by electrospray, matrix-assisted laser desorption/ionization (MALDI) and atmospheric pressure MALDI mass spectrometry."
Wolfender J.L., Chu F., Ball H., Wolfender F., Fainzilber M., Baldwin M.A., Burlingame A.L.
J. Mass Spectrom. 34:447-454(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SULFATION AT TYR-15.
[3]"Single-residue alteration in alpha-conotoxin PnIA switches its nAChR subtype selectivity."
Luo S., Nguyen T.A., Cartier G.E., Olivera B.M., Yoshikami D., McIntosh J.M.
Biochemistry 38:14542-14548(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SYNTHESIS, MUTAGENESIS OF ALA-10 AND ASN-11.
[4]"The 1.1 A crystal structure of the neuronal acetylcholine receptor antagonist, alpha-conotoxin PnIA from Conus pennaceus."
Hu S.-H., Gehrmann J., Guddat L.W., Alewood P.F., Craik D.J., Martin J.L.
Structure 4:417-423(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS), DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

PIRA54877.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1PENX-ray1.10A1-16[»]
2BR8X-ray2.40F/G/H/I/J1-16[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60493N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

ConoServer75. Pni1.
51. PnIA.

Family and domain databases

InterProIPR018072. Conotoxin_a-typ_CS.
[Graphical view]
PROSITEPS60014. ALPHA_CONOTOXIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP50984.

Entry information

Entry nameCA1A_CONPE
AccessionPrimary (citable) accession number: P50984
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 22, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references