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P50982

- CA1_CONER

UniProt

P50982 - CA1_CONER

Protein

Alpha-conotoxin EI

Gene
N/A
Organism
Conus ermineus (Atlantic fish-hunting cone)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. Blocks mammalian nAChR composed of alpha-1/gamma and alpha-1/delta subunits. Blocks central nervous system nAChR composed of alpha-4/beta-2 subunits and peripheral nervous system nAChR composed of alpha-3/beta-4 subunits. Low toxin concentrations potentiate currents in muscle nAChR composed of alpha-1/beta-1/gamma/delta subunits and central nervous system nAChR composed of alpha-4/beta-2 subunits, but not peripheral nervous system nAChR composed of alpha-3/beta-4 subunits.2 Publications

    GO - Molecular functioni

    1. acetylcholine receptor inhibitor activity Source: UniProtKB

    GO - Biological processi

    1. negative regulation of receptor activity Source: GOC
    2. pathogenesis Source: UniProtKB

    Keywords - Molecular functioni

    Acetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-conotoxin EI
    OrganismiConus ermineus (Atlantic fish-hunting cone)
    Taxonomic identifieri55423 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

    Organism-specific databases

    ConoServeri50. EI.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB
    2. other organism postsynaptic membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Propeptidei17 – 43271 PublicationPRO_0000392693Add
    BLAST
    Peptidei44 – 6118Alpha-conotoxin EIPRO_0000044458Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei46 – 4614-hydroxyproline2 Publications
    Disulfide bondi47 ↔ 53
    Disulfide bondi48 ↔ 61
    Modified residuei61 – 611Cysteine amide2 Publications

    Keywords - PTMi

    Amidation, Disulfide bond, Hydroxylation

    Expressioni

    Tissue specificityi

    Expressed by the venom duct.

    Structurei

    Secondary structure

    1
    62
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi46 – 494
    Helixi52 – 554
    Helixi58 – 603

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K64NMR-A44-61[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP50982.

    Family & Domainsi

    Domaini

    The cysteine framework is I (CC-C-C). Alpha4/7 pattern.

    Sequence similaritiesi

    Belongs to the conotoxin A superfamily.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR009958. Conotoxin_a-typ.
    IPR018072. Conotoxin_a-typ_CS.
    [Graphical view]
    PfamiPF07365. Toxin_8. 1 hit.
    [Graphical view]
    PROSITEiPS60014. ALPHA_CONOTOXIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P50982-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFTVFLLVVL ATTVGSFTLD RASDGRDAAA NDKASDLIAL TARRDPCCYH   50
    PTCNMSNPQI CG 62
    Length:62
    Mass (Da):6,637
    Last modified:March 23, 2010 - v2
    Checksum:i7D3E9D3F46B52186
    GO

    Mass spectrometryi

    Molecular mass is 2092.9 Da from positions 44 - 61. Determined by LSI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BD394990 Unassigned DNA. No translation available.
    PIRiA58589.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BD394990 Unassigned DNA. No translation available.
    PIRi A58589.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K64 NMR - A 44-61 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    ConoServeri 50. EI.

    Miscellaneous databases

    EvolutionaryTracei P50982.

    Family and domain databases

    InterProi IPR009958. Conotoxin_a-typ.
    IPR018072. Conotoxin_a-typ_CS.
    [Graphical view ]
    Pfami PF07365. Toxin_8. 1 hit.
    [Graphical view ]
    PROSITEi PS60014. ALPHA_CONOTOXIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE.
    2. "Alpha-conotoxin EI, a new nicotinic acetylcholine receptor antagonist with novel selectivity."
      Martinez J.S., Olivera B.M., Gray W.R., Craig A.G., Groebe D.R., Abramson S.N., McIntosh J.M.
      Biochemistry 34:14519-14526(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 44-61, FUNCTION, SYNTHESIS, DISULFIDE BONDS, HYDROXYLATION AT PRO-46, AMIDATION AT CYS-61, MASS SPECTROMETRY.
      Tissue: Venom.
    3. "Novel alpha-conotoxins from Conus spurius and the alpha-conotoxin EI share high-affinity potentiation and low-affinity inhibition of nicotinic acetylcholine receptors."
      Lopez-Vera E., Aguilar M.B., Schiavon E., Marinzi C., Ortiz E., Restano Cassulini R., Batista C.V.F., Possani L.D., Heimer de la Cotera E.P., Peri F., Becerril B., Wanke E.
      FEBS J. 274:3972-3985(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Solution conformation of alpha-conotoxin EI, a neuromuscular toxin specific for the alpha 1/delta subunit interface of torpedo nicotinic acetylcholine receptor."
      Park K.-H., Suk J.-E., Jacobsen R., Gray W.R., McIntosh J.M., Han K.-H.
      J. Biol. Chem. 276:49028-49033(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 44-61, HYDROXYLATION AT PRO-46, AMIDATION AT CYS-61, DISULFIDE BONDS.

    Entry informationi

    Entry nameiCA1_CONER
    AccessioniPrimary (citable) accession number: P50982
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: March 23, 2010
    Last modified: October 1, 2014
    This is version 61 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3