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P50982 (CA1_CONER) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-conotoxin EI
OrganismConus ermineus (Atlantic fish-hunting cone)
Taxonomic identifier55423 [NCBI]
Taxonomic lineageEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Protein attributes

Sequence length62 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. Blocks mammalian nAChR composed of alpha-1/gamma and alpha-1/delta subunits. Blocks central nervous system nAChR composed of alpha-4/beta-2 subunits and peripheral nervous system nAChR composed of alpha-3/beta-4 subunits. Low toxin concentrations potentiate currents in muscle nAChR composed of alpha-1/beta-1/gamma/delta subunits and central nervous system nAChR composed of alpha-4/beta-2 subunits, but not peripheral nervous system nAChR composed of alpha-3/beta-4 subunits. Ref.2 Ref.3

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom duct.

Domain

The cysteine framework is I (CC-C-C).

Sequence similarities

Belongs to the conotoxin A superfamily.

Mass spectrometry

Molecular mass is 2092.9 Da from positions 44 - 61. Determined by LSI. Ref.2

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Potential
Propeptide17 – 4327
PRO_0000392693
Peptide44 – 6118Alpha-conotoxin EI Ref.2
PRO_0000044458

Amino acid modifications

Modified residue4614-hydroxyproline Ref.2 Ref.4
Modified residue611Cysteine amide Ref.2 Ref.4
Disulfide bond47 ↔ 53 Ref.2 Ref.4
Disulfide bond48 ↔ 61 Ref.2 Ref.4

Secondary structure

....... 62
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P50982 [UniParc].

Last modified March 23, 2010. Version 2.
Checksum: 7D3E9D3F46B52186

FASTA626,637
        10         20         30         40         50         60 
MFTVFLLVVL ATTVGSFTLD RASDGRDAAA NDKASDLIAL TARRDPCCYH PTCNMSNPQI 


CG 

« Hide

References

[1]"Alpha conotoxin peptides."
Olivera B.M., Layer R.T., Watkins M., Hillyard D.R., Mcintosh M.J., Jones R.M., Schoenfeld R.
Patent number JP2003525582, 02-SEP-2003
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Alpha-conotoxin EI, a new nicotinic acetylcholine receptor antagonist with novel selectivity."
Martinez J.S., Olivera B.M., Gray W.R., Craig A.G., Groebe D.R., Abramson S.N., McIntosh J.M.
Biochemistry 34:14519-14526(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 44-61, FUNCTION, SYNTHESIS, DISULFIDE BONDS, HYDROXYLATION AT PRO-46, AMIDATION AT CYS-61, MASS SPECTROMETRY.
Tissue: Venom.
[3]"Novel alpha-conotoxins from Conus spurius and the alpha-conotoxin EI share high-affinity potentiation and low-affinity inhibition of nicotinic acetylcholine receptors."
Lopez-Vera E., Aguilar M.B., Schiavon E., Marinzi C., Ortiz E., Restano Cassulini R., Batista C.V.F., Possani L.D., Heimer de la Cotera E.P., Peri F., Becerril B., Wanke E.
FEBS J. 274:3972-3985(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Solution conformation of alpha-conotoxin EI, a neuromuscular toxin specific for the alpha 1/delta subunit interface of torpedo nicotinic acetylcholine receptor."
Park K.-H., Suk J.-E., Jacobsen R., Gray W.R., McIntosh J.M., Han K.-H.
J. Biol. Chem. 276:49028-49033(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 44-61, HYDROXYLATION AT PRO-46, AMIDATION AT CYS-61, DISULFIDE BONDS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BD394990 Unassigned DNA. No translation available.
PIRA58589.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1K64NMR-A44-61[»]
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

ConoServer50. EI.

Family and domain databases

InterProIPR009958. Conotoxin_a-typ.
IPR018072. Conotoxin_a-typ_CS.
[Graphical view]
PfamPF07365. Toxin_8. 1 hit.
[Graphical view]
PROSITEPS60014. ALPHA_CONOTOXIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP50982.

Entry information

Entry nameCA1_CONER
AccessionPrimary (citable) accession number: P50982
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 23, 2010
Last modified: April 16, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references