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P50982

- CA1_CONER

UniProt

P50982 - CA1_CONER

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Protein

Alpha-conotoxin EI

Gene
N/A
Organism
Conus ermineus (Atlantic fish-hunting cone)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. Blocks mammalian nAChR composed of alpha-1/gamma and alpha-1/delta subunits. Blocks central nervous system nAChR composed of alpha-4/beta-2 subunits and peripheral nervous system nAChR composed of alpha-3/beta-4 subunits. Low toxin concentrations potentiate currents in muscle nAChR composed of alpha-1/beta-1/gamma/delta subunits and central nervous system nAChR composed of alpha-4/beta-2 subunits, but not peripheral nervous system nAChR composed of alpha-3/beta-4 subunits.2 Publications

GO - Molecular functioni

  1. acetylcholine receptor inhibitor activity Source: UniProtKB

GO - Biological processi

  1. negative regulation of receptor activity Source: GOC
  2. pathogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-conotoxin EI
OrganismiConus ermineus (Atlantic fish-hunting cone)
Taxonomic identifieri55423 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Organism-specific databases

ConoServeri50. EI.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
  2. other organism postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Propeptidei17 – 43271 PublicationPRO_0000392693Add
BLAST
Peptidei44 – 6118Alpha-conotoxin EIPRO_0000044458Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei46 – 4614-hydroxyproline2 Publications
Disulfide bondi47 ↔ 53
Disulfide bondi48 ↔ 61
Modified residuei61 – 611Cysteine amide2 Publications

Keywords - PTMi

Amidation, Disulfide bond, Hydroxylation

Expressioni

Tissue specificityi

Expressed by the venom duct.

Structurei

Secondary structure

1
62
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi46 – 494Combined sources
Helixi52 – 554Combined sources
Helixi58 – 603Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K64NMR-A44-61[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50982.

Family & Domainsi

Domaini

The cysteine framework is I (CC-C-C). Alpha4/7 pattern.

Sequence similaritiesi

Belongs to the conotoxin A superfamily.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR009958. Conotoxin_a-typ.
IPR018072. Conotoxin_a-typ_CS.
[Graphical view]
PfamiPF07365. Toxin_8. 1 hit.
[Graphical view]
PROSITEiPS60014. ALPHA_CONOTOXIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50982-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFTVFLLVVL ATTVGSFTLD RASDGRDAAA NDKASDLIAL TARRDPCCYH
60
PTCNMSNPQI CG
Length:62
Mass (Da):6,637
Last modified:March 23, 2010 - v2
Checksum:i7D3E9D3F46B52186
GO

Mass spectrometryi

Molecular mass is 2092.9 Da from positions 44 - 61. Determined by LSI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BD394990 Unassigned DNA. No translation available.
PIRiA58589.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BD394990 Unassigned DNA. No translation available.
PIRi A58589.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1K64 NMR - A 44-61 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

ConoServeri 50. EI.

Miscellaneous databases

EvolutionaryTracei P50982.

Family and domain databases

InterProi IPR009958. Conotoxin_a-typ.
IPR018072. Conotoxin_a-typ_CS.
[Graphical view ]
Pfami PF07365. Toxin_8. 1 hit.
[Graphical view ]
PROSITEi PS60014. ALPHA_CONOTOXIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE.
  2. "Alpha-conotoxin EI, a new nicotinic acetylcholine receptor antagonist with novel selectivity."
    Martinez J.S., Olivera B.M., Gray W.R., Craig A.G., Groebe D.R., Abramson S.N., McIntosh J.M.
    Biochemistry 34:14519-14526(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 44-61, FUNCTION, SYNTHESIS, DISULFIDE BONDS, HYDROXYLATION AT PRO-46, AMIDATION AT CYS-61, MASS SPECTROMETRY.
    Tissue: Venom.
  3. "Novel alpha-conotoxins from Conus spurius and the alpha-conotoxin EI share high-affinity potentiation and low-affinity inhibition of nicotinic acetylcholine receptors."
    Lopez-Vera E., Aguilar M.B., Schiavon E., Marinzi C., Ortiz E., Restano Cassulini R., Batista C.V.F., Possani L.D., Heimer de la Cotera E.P., Peri F., Becerril B., Wanke E.
    FEBS J. 274:3972-3985(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Solution conformation of alpha-conotoxin EI, a neuromuscular toxin specific for the alpha 1/delta subunit interface of torpedo nicotinic acetylcholine receptor."
    Park K.-H., Suk J.-E., Jacobsen R., Gray W.R., McIntosh J.M., Han K.-H.
    J. Biol. Chem. 276:49028-49033(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 44-61, HYDROXYLATION AT PRO-46, AMIDATION AT CYS-61, DISULFIDE BONDS.

Entry informationi

Entry nameiCA1_CONER
AccessioniPrimary (citable) accession number: P50982
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 23, 2010
Last modified: October 29, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3