P50978 (LACG_STRMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phospho-beta-galactosidase
EC=3.2.1.85

Alternative name(s):
Beta-D-phosphogalactoside galactohydrolase
Short name=PGALase
P-beta-Gal
Short name=PBG

Gene names

Name:	lacG
Ordered Locus Names:	SMU_1490

Organism

Streptococcus mutans serotype c (strain ATCC 700610 / UA159) [Complete proteome] [HAMAP]

Taxonomic identifier

210007 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus mutans

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol. HAMAP-Rule MF_01574
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. HAMAP-Rule MF_01574
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lactose catabolic process via tagatose-6-phosphate Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phospho-beta-galactosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	6-phospho-beta-galactosidase HAMAP-Rule MF_01574		PRO_0000063893
Sites
	Active site	160	1	Proton donor By similarity
	Active site	375	1	Nucleophile By similarity
Experimental info
	Sequence conflict	150	1	P → S in AAA16450. Ref.1
	Sequence conflict	300	1	M → I in AAA16450. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P50978 [UniParc]. Last modified November 28, 2002. Version 2. Checksum: FEDBDCE1E23D84F7	FASTA	468	53,762
	10 20 30 40 50 60 MSKTLPKDFI FGGATAAYQA EGATHADGKG PVAWDKYLED NYWYTAEPAS DFYHQYPVDL 70 80 90 100 110 120 KLAEEFGVNG IRISIAWSRI FPKGYGAVNP KGLAFYHNLF AECHKRHVEP FVTLHHFDTP 130 140 150 160 170 180 EALHSNGDFL NRENIEHFVN YAEFCFKEFP EVNYWTTFNE IGPIGDGQYL VGKFPPGIQY 190 200 210 220 230 240 DLAKVFQSHH NMMVAHSKAV KLFKDGGYSG EIGVVHALPT KYPYDPNNPA DIRAAELEDI 250 260 270 280 290 300 IHNKFILDAT YLGKYSEKTM EGVNHILAVN GGQLDLREED FAALEAAKDL NDFLGINYYM 310 320 330 340 350 360 SDWMRAFDGE TEITHNAKGE KGSSKYQIKG VGRREAPVNV PKTDWDWIIY PQGLYDQIMR 370 380 390 400 410 420 VKQDYPNYKK IYITENGLGY KDEFVNHTVY DDARIDYVKK HLEVLSDAIA DGANVKGYFI 430 440 450 460 WSLMDVFSWS NGYEKRYGLF YVDFDTQERY PKKSAYWYKK LAETQIID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, characterization and nucleotide sequence of the Streptococcus mutans lactose-specific enzyme II (lacE) gene of the PTS and the phospho-beta-galactosidase (lacG) gene." Honeyman A.L., Curtiss R. III J. Gen. Microbiol. 139:2685-2694(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 700611 / UA130 / Serotype c.
[2]	"Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen." Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B., Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S., Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J. Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700610 / UA159.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L18993 Unassigned DNA. Translation: AAA16450.1. AE014133 Genomic DNA. Translation: AAN59144.1.
RefSeq	NP_721838.1. NC_004350.2.
3D structure databases
ProteinModelPortal	P50978.
SMR	P50978. Positions 1-468.
ModBase	Search...
Protein-protein interaction databases
STRING	210007.SMU.1490.
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN59144; AAN59144; SMU_1490.
GeneID	1028734.
KEGG	smu:SMU_1490.
PATRIC	19664995. VBIStrMut61772_1326.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2723.
KO	K01220.
OMA	FKDGGYS.
ProtClustDB	PRK13511.
Enzyme and pathway databases
SABIO-RK	P50978.
UniPathway	UPA00542; UER00605.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
HAMAP	MF_01574. LacG.
InterPro	IPR005928. 6P-beta-galactosidase. IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10353. PTHR10353. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR01233. lacG. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LACG_STRMU

Accession

Primary (citable) accession number: P50978

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 28, 2002
Last modified:	May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program