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Protein

6-phospho-beta-galactosidase

Gene

lacG

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol.UniRule annotation

Pathway: lactose degradation

This protein is involved in step 1 of the subpathway that synthesizes D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 6-phospho-beta-galactosidase (lacG)
This subpathway is part of the pathway lactose degradation, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate, the pathway lactose degradation and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei160 – 1601Proton donorUniRule annotation
Active sitei375 – 3751NucleophileUniRule annotation

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: CACAO
  • lactose catabolic process via tagatose-6-phosphate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciSMUT210007:GC7Z-1399-MONOMER.
SABIO-RKP50978.
UniPathwayiUPA00542; UER00605.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-beta-galactosidaseUniRule annotation (EC:3.2.1.85UniRule annotation)
Alternative name(s):
Beta-D-phosphogalactoside galactohydrolaseUniRule annotation
Short name:
PGALaseUniRule annotation
P-beta-GalUniRule annotation
Short name:
PBGUniRule annotation
Gene namesi
Name:lacGUniRule annotation
Ordered Locus Names:SMU_1490
OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
Taxonomic identifieri210007 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000002512 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4684686-phospho-beta-galactosidasePRO_0000063893Add
BLAST

Proteomic databases

PRIDEiP50978.

Interactioni

Protein-protein interaction databases

STRINGi210007.SMU_1490.

Structurei

3D structure databases

ProteinModelPortaliP50978.
SMRiP50978. Positions 1-468.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2723.
KOiK01220.
OMAiHARIVNA.
OrthoDBiEOG60658J.
PhylomeDBiP50978.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
HAMAPiMF_01574. LacG.
InterProiIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01233. lacG. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50978-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKTLPKDFI FGGATAAYQA EGATHADGKG PVAWDKYLED NYWYTAEPAS
60 70 80 90 100
DFYHQYPVDL KLAEEFGVNG IRISIAWSRI FPKGYGAVNP KGLAFYHNLF
110 120 130 140 150
AECHKRHVEP FVTLHHFDTP EALHSNGDFL NRENIEHFVN YAEFCFKEFP
160 170 180 190 200
EVNYWTTFNE IGPIGDGQYL VGKFPPGIQY DLAKVFQSHH NMMVAHSKAV
210 220 230 240 250
KLFKDGGYSG EIGVVHALPT KYPYDPNNPA DIRAAELEDI IHNKFILDAT
260 270 280 290 300
YLGKYSEKTM EGVNHILAVN GGQLDLREED FAALEAAKDL NDFLGINYYM
310 320 330 340 350
SDWMRAFDGE TEITHNAKGE KGSSKYQIKG VGRREAPVNV PKTDWDWIIY
360 370 380 390 400
PQGLYDQIMR VKQDYPNYKK IYITENGLGY KDEFVNHTVY DDARIDYVKK
410 420 430 440 450
HLEVLSDAIA DGANVKGYFI WSLMDVFSWS NGYEKRYGLF YVDFDTQERY
460
PKKSAYWYKK LAETQIID
Length:468
Mass (Da):53,762
Last modified:November 28, 2002 - v2
Checksum:iFEDBDCE1E23D84F7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti150 – 1501P → S in AAA16450 (PubMed:8277252).Curated
Sequence conflicti300 – 3001M → I in AAA16450 (PubMed:8277252).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L18993 Unassigned DNA. Translation: AAA16450.1.
AE014133 Genomic DNA. Translation: AAN59144.1.
RefSeqiNP_721838.1. NC_004350.2.
WP_002263058.1. NC_004350.2.

Genome annotation databases

EnsemblBacteriaiAAN59144; AAN59144; SMU_1490.
GeneIDi1028734.
KEGGismu:SMU_1490.
PATRICi19664995. VBIStrMut61772_1326.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L18993 Unassigned DNA. Translation: AAA16450.1.
AE014133 Genomic DNA. Translation: AAN59144.1.
RefSeqiNP_721838.1. NC_004350.2.
WP_002263058.1. NC_004350.2.

3D structure databases

ProteinModelPortaliP50978.
SMRiP50978. Positions 1-468.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi210007.SMU_1490.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEiP50978.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN59144; AAN59144; SMU_1490.
GeneIDi1028734.
KEGGismu:SMU_1490.
PATRICi19664995. VBIStrMut61772_1326.

Phylogenomic databases

eggNOGiCOG2723.
KOiK01220.
OMAiHARIVNA.
OrthoDBiEOG60658J.
PhylomeDBiP50978.

Enzyme and pathway databases

UniPathwayiUPA00542; UER00605.
BioCyciSMUT210007:GC7Z-1399-MONOMER.
SABIO-RKP50978.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
HAMAPiMF_01574. LacG.
InterProiIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01233. lacG. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, characterization and nucleotide sequence of the Streptococcus mutans lactose-specific enzyme II (lacE) gene of the PTS and the phospho-beta-galactosidase (lacG) gene."
    Honeyman A.L., Curtiss R. III
    J. Gen. Microbiol. 139:2685-2694(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 700611 / UA130 / Serotype c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700610 / UA159.

Entry informationi

Entry nameiLACG_STRMU
AccessioniPrimary (citable) accession number: P50978
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 28, 2002
Last modified: June 24, 2015
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.