Reviewed, UniProtKB/Swiss-Prot P50977 (LACG_LACAC)

Names and origin

Protein names

Recommended name:
    6-phospho-beta-galactosidase
    EC=3.2.1.85
Alternative name(s):
    Beta-D-phosphogalactoside galactohydrolase
      Short name=PGALase
    P-beta-Gal
      Short name=PBG

Gene names

Name:	lacG
Synonyms:	pbg

Organism

Lactobacillus acidophilus

Taxonomic identifier

1579 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A 6-phospho-beta-D-galactoside + H(2)O = 6-phospho-D-galactose + an alcohol.
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	lactose catabolic process via tagatose-6-phosphate Inferred from electronic annotation. Source: InterPro
Molecular function	6-phospho-beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro galactosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	6-phospho-beta-galactosidase		PRO_0000063881
Sites
	Active site	160	1	Proton donor By similarity
	Active site	375	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50977-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A7D17EADEC19246A	FASTA	473	54,009
	10 20 30 40 50 60 MTKTLPKDFI FGGATAAYQA EGATKTDGKG RVAWDKFLEE NFWYKGDPAS DFYHNYVEDL 70 80 90 100 110 120 ELAEKFGGNV IRISIAWSRI FPNGDGEVKP NGVDFYHKLF AECDARHVEP FVTLHHFDTP 130 140 150 160 170 180 EGLHEDGDFL THEKMDDFVE YADYCFKEFP EVKYWITINE IRSVAVDQYI IGNFPPADTF 190 200 210 220 230 240 GFDKMFQTHH NQMVGHARAV KLFKHDGSKG EIGIVHALQT NYPFNESNPA DIGAAELEDL 250 260 270 280 290 300 LDNKFLVDGT FVGKYPQETM EAVKDILAAN HGGEFNIEDE FKAIDAAKDV QDFVGVDYYL 310 320 330 340 350 360 SEWMRAYDGK SEITHNGTGD KGTSKVQVKG VGEEKLPDGI ETTDWDWLIY PQGLYDKIMR 370 380 390 400 410 420 VKNDYPNIHK VYITENGIGF KDTVPDNEET DKTVHDDARI DYVKQHLEVI ADAIADGANV 430 440 450 460 470 KGYFIWSLMD VFTWTNGYTK RYGLFYVDFD TQDRYPSKTA DWFKNLAETH IIE

References

[1]	"Isolation and structural analysis of the phospho-beta-galactosidase gene from Lactobacillus acidophilus." Kanatani K., Oshimura M. J. Ferment. Bioeng. 78:123-129(1994) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LTF42.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D37882 Genomic DNA. Translation: BAA07122.1.
3D structure databases
HSSP	HSSP built from PDB template 1PBG based on UniProtKB P11546.
SMR	P50977. Positions 1-473.
ModBase	Search...
Family and domain databases
HAMAP	MF_01574. [Tree]
InterPro	IPR001360. Glyco_hydro_1. IPR013781. Glyco_hydro_sub_cat. IPR005928. LacG. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
TIGRFAMs	TIGR01233. lacG. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LACG_LACAC

Accession

Primary (citable) accession number: P50977

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)