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Protein

PTS system lactose-specific EIICB component

Gene

lacE

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II LacEF PTS system is involved in lactose transport.By similarity1 Publication

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + lactose(Side 1) = [protein]-L-histidine + lactose 6'-phosphate(Side 2).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei472Phosphocysteine intermediate; for EIIB activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system lactose-specific EIICB component1 Publication
Alternative name(s):
EIICB-Lac1 Publication
Short name:
EII-Lac1 Publication
Including the following 2 domains:
PTS system lactose-specific EIIC component1 Publication
Alternative name(s):
Lactose permease IIC component1 Publication
PTS system lactose-specific EIIB component1 Publication (EC:2.7.1.207By similarity)
Alternative name(s):
Lactose-specific phosphotransferase enzyme IIB component1 Publication
Gene namesi
Name:lacE1 Publication
Ordered Locus Names:SMU_1491
OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
Taxonomic identifieri210007 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000002512 Componenti: Chromosome

Subcellular locationi

P50976:
  • Cell membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei30 – 50HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei65 – 85HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei103 – 123HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei128 – 148HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei183 – 203HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei222 – 242HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei246 – 266HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei283 – 303HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei339 – 359HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei381 – 401HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865951 – 568PTS system lactose-specific EIICB componentAdd BLAST568

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei472Phosphocysteine; by EIIAPROSITE-ProRule annotationBy similarity1

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

Induced by lactose, galactose and galactose-6-P. Repressed by glucose.By similarity

Interactioni

Protein-protein interaction databases

STRINGi210007.SMU_1491.

Structurei

3D structure databases

ProteinModelPortaliP50976.
SMRiP50976.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 409PTS EIIC type-3PROSITE-ProRule annotationAdd BLAST402
Domaini465 – 568PTS EIIB type-3PROSITE-ProRule annotationAdd BLAST104

Domaini

The EIIC type-3 domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation
The PTS EIIB type-3 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CK1. Bacteria.
COG1455. LUCA.
KOiK02787.
K02788.
OMAiISAMPFM.
PhylomeDBiP50976.

Family and domain databases

InterProiView protein in InterPro
IPR036095. PTS_EIIB-like_sf.
IPR003501. PTS_EIIB_2/3.
IPR013012. PTS_EIIB_3.
IPR003352. PTS_EIIC.
IPR004501. PTS_EIIC_3.
IPR004801. PTS_IIC_lac_spec.
PfamiView protein in Pfam
PF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00394. lac_pts_IIC. 1 hit.
TIGR00410. lacE. 1 hit.
TIGR00853. pts-lac. 1 hit.
PROSITEiView protein in PROSITE
PS51100. PTS_EIIB_TYPE_3. 1 hit.
PS51105. PTS_EIIC_TYPE_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P50976-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTLIAQIEK GKPFFEKISR NIYLRAIRDG FISAMPVILF SSIFLLIAYV
60 70 80 90 100
PNIFGFTWPK GIENMLMTPY NYTMGIIGFL VAGTTAKSLT DSMNRQLEKT
110 120 130 140 150
NQINFISTML ASMAGFLIMA ADPAKEGGFL SAFMGTKGLL TAFIAAFITV
160 170 180 190 200
NVYKICVKNN VTIRMPEEVP PNISQVFKDI FPFAFSIIIL YAIQLAIKAV
210 220 230 240 250
IGVNVAQSIG TLLAPLFSAA DGYLGITIIF GAYALFWFVG IHGPSIVEPA
260 270 280 290 300
IAAITYSNVE LNAHLIHAGQ HADKVITSGT QMFIVTMGGT GATLVVPFMF
310 320 330 340 350
MWLCKSKRNK AIGRASVVPT FFGVNEPILF GAPIVLNPVF FIPFILAPIV
360 370 380 390 400
NVWIFKFFVD TLGMNSFFAN LPWTTPGPIG IVLGTGFAVL SFVLAALLIL
410 420 430 440 450
VDTVIYYPFV KVYDEQILAE EAEGKSSSDA LKEKVAANFD TKKADAILEG
460 470 480 490 500
AESKEEPATH AITEETNVLV LCAGGGTSGL LANALNKAAE EYGAPVKAAA
510 520 530 540 550
GSYGAHREIL DQYQLVILAP QVASNYEDMK AETDKLGIKL AKTEGAQYIG
560
LTRDGKGALA FVEEQFKD
Length:568
Mass (Da):61,420
Last modified:October 1, 1996 - v1
Checksum:iA129FDD14282EAAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L18993 Unassigned DNA. Translation: AAA16449.1.
AE014133 Genomic DNA. Translation: AAN59145.1.
RefSeqiNP_721839.1. NC_004350.2.
WP_002263057.1. NC_004350.2.

Genome annotation databases

EnsemblBacteriaiAAN59145; AAN59145; SMU_1491.
GeneIDi1028733.
KEGGismu:SMU_1491.
PATRICifig|210007.7.peg.1327.

Similar proteinsi

Entry informationi

Entry nameiPTLCB_STRMU
AccessioniPrimary (citable) accession number: P50976
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 25, 2017
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome