Reviewed, UniProtKB/Swiss-Prot P50971 (TRXB_EUBAC)

Names and origin

Protein names

Recommended name:
    Thioredoxin reductase
      Short name=TRXR
    EC=1.8.1.9

Gene names

Name:

trxB

Organism

Eubacterium acidaminophilum

Taxonomic identifier

1731 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Eubacteriaceae › Eubacterium

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW removal of superoxide radicals Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro thioredoxin-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Thioredoxin reductase		PRO_0000166731
Regions
	Nucleotide binding	34 – 41	8	FAD By similarity
	Nucleotide binding	282 – 291	10	FAD By similarity
Amino acid modifications
	Disulfide bond	134 ↔ 137		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50971-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 145B0EABB2B8A7FA	FASTA	315	34,034
	10 20 30 40 50 60 MENVYDLAII GSGPAGLAAA LYGARAKMKT IMIEGQKVGG QIVITHEVAN YPGSVREATG 70 80 90 100 110 120 PSLIERMEEQ ANEFGAEKVM DKIVDVDLDG KIKVIKGEKA EYKAKSVILA TGAAPRLAGC 130 140 150 160 170 180 PGEQELTGKG VSYCATCDAD FFEDMEVFVV GGGDTAVEEA MYLAKFARKV TIVHRRDELR 190 200 210 220 230 240 AAKSIQEKAF KNPKLDFMWN SAIEEIKGDG IVESAVFKNL VTGETTEYFA NEEDGTFGIF 250 260 270 280 290 300 VFIGYIPKSD VFKGKITLDD AGYIITDDNM KTNVEGVFAA GDIRVKSLRQ VVTACADGAI 310 AATQAEKYVE ANFEE

References

[1]	"Components of glycine reductase from Eubacterium acidaminophilum. Cloning, sequencing and identification of the genes for thioredoxin reductase, thioredoxin and selenoprotein PA." Luebbers M., Andreesen J.R. Eur. J. Biochem. 217:791-798(1993) [PubMed: 8223622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49065 / DSM 3953 / al-2.
[2]	Andreesen J.R. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 275.
[3]	"Isolation of an atypically small lipoamide dehydrogenase involved in the glycine decarboxylase complex from Eubacterium acidaminophilum." Freundenberg W., Dietrichs D., Lebertz H., Andreesen J.R. J. Bacteriol. 171:1346-1354(1989) [PubMed: 2537814] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-54.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L04500 Genomic DNA. Translation: AAB93303.1.
PIR	D35156. S38988.
3D structure databases
HSSP	HSSP built from PDB template 1TDE based on UniProtKB P09625.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.8.1.9. 256975.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR005982. Thioredox_Rdtase. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01292. TRX_reduct. 1 hit.
PROSITE	PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRXB_EUBAC

Accession

Primary (citable) accession number: P50971

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)