P50946 (OCA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative tyrosine-protein phosphatase OCA1
EC=3.1.3.48

Alternative name(s):
Oxidant-induced cell-cycle arrest protein 1

Gene names

Name:	OCA1
Ordered Locus Names:	YNL099C
ORF Names:	N2194

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative tyrosine-protein phosphatase required for protection against superoxide stress. Involved in cell-cycle delay in response to linoleic acid hydroperoxide (LoaOOH). Ref.6 Ref.7 Ref.11
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subcellular location	Cytoplasm Ref.8.
Miscellaneous	Present with 2310 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to oxidative stress Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.8. Source: SGD
Molecular_function	protein tyrosine phosphatase activity Inferred from sequence or structural similarity Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
OCA2	P53949	6	EBI-28814,EBI-28725
SIW14	P53965	5	EBI-28814,EBI-28668

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Putative tyrosine-protein phosphatase OCA1		PRO_0000203439
Sites
	Active site	168	1	Phosphocysteine intermediate By similarity
Natural variations
	Natural variant	66	1	R → C in strain: SK1. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P50946 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 114DD87CBA2FAEBF	FASTA	238	27,205
	10 20 30 40 50 60 MTSKVGEYED VPEDESRLTE ENVSVPEEEV EDEDEEEDDD DDHIYINEET ESGREKVLVS 70 80 90 100 110 120 HAPQERIVPP LNFCPVERYL YRSGQPSPVN FPFLLNLKLK TIIWLSNEEP QDTLLEFCDT 130 140 150 160 170 180 HRINLQFAAI NPDAGEDDNP WDGLTEHSII NVLQTIVTQE NYPLLVCCGM GRHRTGTVIG 190 200 210 220 230 CLRRIMGWNL ASVSEEYRRF TGSRGGRILV ELLIEAFDTN LVKIDKNKAP SWLLTALE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Quantitative trait loci mapped to single-nucleotide resolution in yeast." Deutschbauer A.M., Davis R.W. Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT CYS-66. Strain: SK1.
[2]	"The sequence of a 21.3 kb DNA fragment from the left arm of yeast chromosome XIV reveals LEU4, MET4, POL1, RAS2, and six new open reading frames." Saiz J.E., Buitrago M.J., Soler A., del Rey F., Revuelta J.L. Yeast 12:403-409(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Gathering STYX: phosphatase-like form predicts functions for unique protein-interaction domains." Wishart M.J., Dixon J.E. Trends Biochem. Sci. 23:301-306(1998) [PubMed] [Europe PMC] [Abstract] Cited for: DOMAIN.
[6]	"Identification of a Saccharomyces cerevisiae gene that is required for G1 arrest in response to the lipid oxidation product linoleic acid hydroperoxide." Alic N., Higgins V.J., Dawes I.W. Mol. Biol. Cell 12:1801-1810(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Lipid hydroperoxides activate the mitogen-activated protein kinase Mpk1p in Saccharomyces cerevisiae." Alic N., Higgins V.J., Pichova A., Breitenbach M., Dawes I.W. J. Biol. Chem. 278:41849-41855(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Proteome survey reveals modularity of the yeast cell machinery." Gavin A.-C., Aloy P., Grandi P., Krause R., Boesche M., Marzioch M., Rau C., Jensen L.J., Bastuck S., Duempelfeld B., Edelmann A., Heurtier M.-A., Hoffman V., Hoefert C., Klein K., Hudak M., Michon A.-M., Schelder M. , Schirle M., Remor M., Rudi T., Hooper S., Bauer A., Bouwmeester T., Casari G., Drewes G., Neubauer G., Rick J.M., Kuster B., Bork P., Russell R.B., Superti-Furga G. Nature 440:631-636(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH OCA2; OCA4; OCA5 AND SIW14.
[11]	"Oxidant-induced cell-cycle delay in Saccharomyces cerevisiae: the involvement of the SWI6 transcription factor." Fong C.S., Temple M.D., Alic N., Chiu J., Durchdewald M., Thorpe G.W., Higgins V.J., Dawes I.W. FEMS Yeast Res. 8:386-399(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ115393 Genomic DNA. Translation: AAZ22508.1. Z50161 Genomic DNA. Translation: CAA90527.1. Z71375 Genomic DNA. Translation: CAA95975.1. BK006947 Genomic DNA. Translation: DAA10446.1.
PIR	S58253.
RefSeq	NP_014300.3. NM_001182937.3.
3D structure databases
ProteinModelPortal	P50946.
SMR	P50946. Positions 67-220.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1991N.
IntAct	P50946. 4 interactions.
MINT	MINT-406344.
STRING	4932.YNL099C.
Proteomic databases
PaxDb	P50946.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL099C; YNL099C; YNL099C.
GeneID	855624.
KEGG	sce:YNL093W. sce:YNL099C.
Organism-specific databases
CYGD	YNL099c.
SGD	S000005043. OCA1.
Phylogenomic databases
eggNOG	COG2365.
GeneTree	ENSGT00530000066535.
HOGENOM	HOG000188365.
KO	K07976.
OMA	VPPLNFC.
OrthoDB	EOG40VZZZ.
Gene expression databases
Genevestigator	P50946.
GermOnline	YNL099C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004861. Tyr_Pase_SIW14-like. [Graphical view]
Pfam	PF03162. Y_phosphatase2. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. False negative. PS50056. TYR_PHOSPHATASE_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	979822.

Entry information

Entry name

OCA1_YEAST

Accession

Primary (citable) accession number: P50946
Secondary accession number(s): D6W180, Q45U02

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program