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P50946

- OCA1_YEAST

UniProt

P50946 - OCA1_YEAST

Protein

Putative tyrosine-protein phosphatase OCA1

Gene

OCA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Putative tyrosine-protein phosphatase required for protection against superoxide stress. Involved in cell-cycle delay in response to linoleic acid hydroperoxide (LoaOOH).3 Publications

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei168 – 1681Phosphocysteine intermediateBy similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein tyrosine phosphatase activity Source: SGD

    GO - Biological processi

    1. cellular response to oxidative stress Source: SGD
    2. peptidyl-tyrosine dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Stress response

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33127-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative tyrosine-protein phosphatase OCA1 (EC:3.1.3.48)
    Alternative name(s):
    Oxidant-induced cell-cycle arrest protein 1
    Gene namesi
    Name:OCA1
    Ordered Locus Names:YNL099C
    ORF Names:N2194
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL099c.
    SGDiS000005043. OCA1.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 238237Putative tyrosine-protein phosphatase OCA1PRO_0000203439Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylthreonine1 Publication
    Modified residuei24 – 241Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP50946.
    PaxDbiP50946.

    Expressioni

    Gene expression databases

    GenevestigatoriP50946.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    OCA2P539495EBI-28814,EBI-28725
    SIW14P539654EBI-28814,EBI-28668

    Protein-protein interaction databases

    BioGridi35724. 123 interactions.
    DIPiDIP-1991N.
    IntActiP50946. 4 interactions.
    MINTiMINT-406344.
    STRINGi4932.YNL099C.

    Structurei

    3D structure databases

    ProteinModelPortaliP50946.
    SMRiP50946. Positions 67-220.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2365.
    GeneTreeiENSGT00530000066535.
    HOGENOMiHOG000188365.
    KOiK18043.
    OMAiKLQRWNL.
    OrthoDBiEOG7Z0K6V.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR029021. Prot-tyrosine_phosphatase-like.
    IPR004861. Tyr_Pase_SIW14-like.
    [Graphical view]
    PfamiPF03162. Y_phosphatase2. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P50946-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSKVGEYED VPEDESRLTE ENVSVPEEEV EDEDEEEDDD DDHIYINEET    50
    ESGREKVLVS HAPQERIVPP LNFCPVERYL YRSGQPSPVN FPFLLNLKLK 100
    TIIWLSNEEP QDTLLEFCDT HRINLQFAAI NPDAGEDDNP WDGLTEHSII 150
    NVLQTIVTQE NYPLLVCCGM GRHRTGTVIG CLRRIMGWNL ASVSEEYRRF 200
    TGSRGGRILV ELLIEAFDTN LVKIDKNKAP SWLLTALE 238
    Length:238
    Mass (Da):27,205
    Last modified:October 1, 1996 - v1
    Checksum:i114DD87CBA2FAEBF
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti66 – 661R → C in strain: SK1. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ115393 Genomic DNA. Translation: AAZ22508.1.
    Z50161 Genomic DNA. Translation: CAA90527.1.
    Z71375 Genomic DNA. Translation: CAA95975.1.
    BK006947 Genomic DNA. Translation: DAA10446.1.
    PIRiS58253.
    RefSeqiNP_014300.3. NM_001182937.3.

    Genome annotation databases

    EnsemblFungiiYNL099C; YNL099C; YNL099C.
    GeneIDi855624.
    KEGGisce:YNL099C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ115393 Genomic DNA. Translation: AAZ22508.1 .
    Z50161 Genomic DNA. Translation: CAA90527.1 .
    Z71375 Genomic DNA. Translation: CAA95975.1 .
    BK006947 Genomic DNA. Translation: DAA10446.1 .
    PIRi S58253.
    RefSeqi NP_014300.3. NM_001182937.3.

    3D structure databases

    ProteinModelPortali P50946.
    SMRi P50946. Positions 67-220.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35724. 123 interactions.
    DIPi DIP-1991N.
    IntActi P50946. 4 interactions.
    MINTi MINT-406344.
    STRINGi 4932.YNL099C.

    Proteomic databases

    MaxQBi P50946.
    PaxDbi P50946.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL099C ; YNL099C ; YNL099C .
    GeneIDi 855624.
    KEGGi sce:YNL099C.

    Organism-specific databases

    CYGDi YNL099c.
    SGDi S000005043. OCA1.

    Phylogenomic databases

    eggNOGi COG2365.
    GeneTreei ENSGT00530000066535.
    HOGENOMi HOG000188365.
    KOi K18043.
    OMAi KLQRWNL.
    OrthoDBi EOG7Z0K6V.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33127-MONOMER.

    Miscellaneous databases

    NextBioi 979822.

    Gene expression databases

    Genevestigatori P50946.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR029021. Prot-tyrosine_phosphatase-like.
    IPR004861. Tyr_Pase_SIW14-like.
    [Graphical view ]
    Pfami PF03162. Y_phosphatase2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Quantitative trait loci mapped to single-nucleotide resolution in yeast."
      Deutschbauer A.M., Davis R.W.
      Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT CYS-66.
      Strain: SK1.
    2. "The sequence of a 21.3 kb DNA fragment from the left arm of yeast chromosome XIV reveals LEU4, MET4, POL1, RAS2, and six new open reading frames."
      Saiz J.E., Buitrago M.J., Soler A., del Rey F., Revuelta J.L.
      Yeast 12:403-409(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Gathering STYX: phosphatase-like form predicts functions for unique protein-interaction domains."
      Wishart M.J., Dixon J.E.
      Trends Biochem. Sci. 23:301-306(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN.
    6. "Identification of a Saccharomyces cerevisiae gene that is required for G1 arrest in response to the lipid oxidation product linoleic acid hydroperoxide."
      Alic N., Higgins V.J., Dawes I.W.
      Mol. Biol. Cell 12:1801-1810(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Lipid hydroperoxides activate the mitogen-activated protein kinase Mpk1p in Saccharomyces cerevisiae."
      Alic N., Higgins V.J., Pichova A., Breitenbach M., Dawes I.W.
      J. Biol. Chem. 278:41849-41855(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. Cited for: INTERACTION WITH OCA2; OCA4; OCA5 AND SIW14.
    11. "Oxidant-induced cell-cycle delay in Saccharomyces cerevisiae: the involvement of the SWI6 transcription factor."
      Fong C.S., Temple M.D., Alic N., Chiu J., Durchdewald M., Thorpe G.W., Higgins V.J., Dawes I.W.
      FEMS Yeast Res. 8:386-399(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiOCA1_YEAST
    AccessioniPrimary (citable) accession number: P50946
    Secondary accession number(s): D6W180, Q45U02
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2310 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3