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P50946 (OCA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative tyrosine-protein phosphatase OCA1

EC=3.1.3.48
Alternative name(s):
Oxidant-induced cell-cycle arrest protein 1
Gene names
Name:OCA1
Ordered Locus Names:YNL099C
ORF Names:N2194
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length238 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Putative tyrosine-protein phosphatase required for protection against superoxide stress. Involved in cell-cycle delay in response to linoleic acid hydroperoxide (LoaOOH). Ref.6 Ref.7 Ref.11

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subcellular location

Cytoplasm Ref.8.

Miscellaneous

Present with 2310 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

OCA2P539495EBI-28814,EBI-28725
SIW14P539654EBI-28814,EBI-28668

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.13
Chain2 – 238237Putative tyrosine-protein phosphatase OCA1
PRO_0000203439

Sites

Active site1681Phosphocysteine intermediate By similarity

Amino acid modifications

Modified residue21N-acetylthreonine Ref.13
Modified residue241Phosphoserine Ref.12

Natural variations

Natural variant661R → C in strain: SK1. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P50946 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 114DD87CBA2FAEBF

FASTA23827,205
        10         20         30         40         50         60 
MTSKVGEYED VPEDESRLTE ENVSVPEEEV EDEDEEEDDD DDHIYINEET ESGREKVLVS 

        70         80         90        100        110        120 
HAPQERIVPP LNFCPVERYL YRSGQPSPVN FPFLLNLKLK TIIWLSNEEP QDTLLEFCDT 

       130        140        150        160        170        180 
HRINLQFAAI NPDAGEDDNP WDGLTEHSII NVLQTIVTQE NYPLLVCCGM GRHRTGTVIG 

       190        200        210        220        230 
CLRRIMGWNL ASVSEEYRRF TGSRGGRILV ELLIEAFDTN LVKIDKNKAP SWLLTALE 

« Hide

References

« Hide 'large scale' references
[1]"Quantitative trait loci mapped to single-nucleotide resolution in yeast."
Deutschbauer A.M., Davis R.W.
Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT CYS-66.
Strain: SK1.
[2]"The sequence of a 21.3 kb DNA fragment from the left arm of yeast chromosome XIV reveals LEU4, MET4, POL1, RAS2, and six new open reading frames."
Saiz J.E., Buitrago M.J., Soler A., del Rey F., Revuelta J.L.
Yeast 12:403-409(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Gathering STYX: phosphatase-like form predicts functions for unique protein-interaction domains."
Wishart M.J., Dixon J.E.
Trends Biochem. Sci. 23:301-306(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN.
[6]"Identification of a Saccharomyces cerevisiae gene that is required for G1 arrest in response to the lipid oxidation product linoleic acid hydroperoxide."
Alic N., Higgins V.J., Dawes I.W.
Mol. Biol. Cell 12:1801-1810(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Lipid hydroperoxides activate the mitogen-activated protein kinase Mpk1p in Saccharomyces cerevisiae."
Alic N., Higgins V.J., Pichova A., Breitenbach M., Dawes I.W.
J. Biol. Chem. 278:41849-41855(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Proteome survey reveals modularity of the yeast cell machinery."
Gavin A.-C., Aloy P., Grandi P., Krause R., Boesche M., Marzioch M., Rau C., Jensen L.J., Bastuck S., Duempelfeld B., Edelmann A., Heurtier M.-A., Hoffman V., Hoefert C., Klein K., Hudak M., Michon A.-M., Schelder M. expand/collapse author list , Schirle M., Remor M., Rudi T., Hooper S., Bauer A., Bouwmeester T., Casari G., Drewes G., Neubauer G., Rick J.M., Kuster B., Bork P., Russell R.B., Superti-Furga G.
Nature 440:631-636(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH OCA2; OCA4; OCA5 AND SIW14.
[11]"Oxidant-induced cell-cycle delay in Saccharomyces cerevisiae: the involvement of the SWI6 transcription factor."
Fong C.S., Temple M.D., Alic N., Chiu J., Durchdewald M., Thorpe G.W., Higgins V.J., Dawes I.W.
FEMS Yeast Res. 8:386-399(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ115393 Genomic DNA. Translation: AAZ22508.1.
Z50161 Genomic DNA. Translation: CAA90527.1.
Z71375 Genomic DNA. Translation: CAA95975.1.
BK006947 Genomic DNA. Translation: DAA10446.1.
PIRS58253.
RefSeqNP_014300.3. NM_001182937.3.

3D structure databases

ProteinModelPortalP50946.
SMRP50946. Positions 67-220.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35724. 123 interactions.
DIPDIP-1991N.
IntActP50946. 4 interactions.
MINTMINT-406344.
STRING4932.YNL099C.

Proteomic databases

MaxQBP50946.
PaxDbP50946.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL099C; YNL099C; YNL099C.
GeneID855624.
KEGGsce:YNL099C.

Organism-specific databases

CYGDYNL099c.
SGDS000005043. OCA1.

Phylogenomic databases

eggNOGCOG2365.
GeneTreeENSGT00530000066535.
HOGENOMHOG000188365.
KOK18043.
OMAKLQRWNL.
OrthoDBEOG7Z0K6V.

Enzyme and pathway databases

BioCycYEAST:G3O-33127-MONOMER.

Gene expression databases

GenevestigatorP50946.

Family and domain databases

Gene3D3.90.190.10. 1 hit.
InterProIPR029021. Prot-tyrosine_phosphatase-like.
IPR004861. Tyr_Pase_SIW14-like.
[Graphical view]
PfamPF03162. Y_phosphatase2. 1 hit.
[Graphical view]
SUPFAMSSF52799. SSF52799. 1 hit.
ProtoNetSearch...

Other

NextBio979822.

Entry information

Entry nameOCA1_YEAST
AccessionPrimary (citable) accession number: P50946
Secondary accession number(s): D6W180, Q45U02
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families