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P50946

- OCA1_YEAST

UniProt

P50946 - OCA1_YEAST

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Protein

Putative tyrosine-protein phosphatase OCA1

Gene
OCA1, YNL099C, N2194
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Putative tyrosine-protein phosphatase required for protection against superoxide stress. Involved in cell-cycle delay in response to linoleic acid hydroperoxide (LoaOOH).3 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei168 – 1681Phosphocysteine intermediate By similarity

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein tyrosine phosphatase activity Source: SGD

GO - Biological processi

  1. cellular response to oxidative stress Source: SGD
  2. peptidyl-tyrosine dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciYEAST:G3O-33127-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative tyrosine-protein phosphatase OCA1 (EC:3.1.3.48)
Alternative name(s):
Oxidant-induced cell-cycle arrest protein 1
Gene namesi
Name:OCA1
Ordered Locus Names:YNL099C
ORF Names:N2194
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL099c.
SGDiS000005043. OCA1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 238237Putative tyrosine-protein phosphatase OCA1PRO_0000203439Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonine1 Publication
Modified residuei24 – 241Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP50946.
PaxDbiP50946.

Expressioni

Gene expression databases

GenevestigatoriP50946.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
OCA2P539495EBI-28814,EBI-28725
SIW14P539654EBI-28814,EBI-28668

Protein-protein interaction databases

BioGridi35724. 123 interactions.
DIPiDIP-1991N.
IntActiP50946. 4 interactions.
MINTiMINT-406344.
STRINGi4932.YNL099C.

Structurei

3D structure databases

ProteinModelPortaliP50946.
SMRiP50946. Positions 67-220.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2365.
GeneTreeiENSGT00530000066535.
HOGENOMiHOG000188365.
KOiK18043.
OMAiKLQRWNL.
OrthoDBiEOG7Z0K6V.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR004861. Tyr_Pase_SIW14-like.
[Graphical view]
PfamiPF03162. Y_phosphatase2. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50946-1 [UniParc]FASTAAdd to Basket

« Hide

MTSKVGEYED VPEDESRLTE ENVSVPEEEV EDEDEEEDDD DDHIYINEET    50
ESGREKVLVS HAPQERIVPP LNFCPVERYL YRSGQPSPVN FPFLLNLKLK 100
TIIWLSNEEP QDTLLEFCDT HRINLQFAAI NPDAGEDDNP WDGLTEHSII 150
NVLQTIVTQE NYPLLVCCGM GRHRTGTVIG CLRRIMGWNL ASVSEEYRRF 200
TGSRGGRILV ELLIEAFDTN LVKIDKNKAP SWLLTALE 238
Length:238
Mass (Da):27,205
Last modified:October 1, 1996 - v1
Checksum:i114DD87CBA2FAEBF
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti66 – 661R → C in strain: SK1. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ115393 Genomic DNA. Translation: AAZ22508.1.
Z50161 Genomic DNA. Translation: CAA90527.1.
Z71375 Genomic DNA. Translation: CAA95975.1.
BK006947 Genomic DNA. Translation: DAA10446.1.
PIRiS58253.
RefSeqiNP_014300.3. NM_001182937.3.

Genome annotation databases

EnsemblFungiiYNL099C; YNL099C; YNL099C.
GeneIDi855624.
KEGGisce:YNL099C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ115393 Genomic DNA. Translation: AAZ22508.1 .
Z50161 Genomic DNA. Translation: CAA90527.1 .
Z71375 Genomic DNA. Translation: CAA95975.1 .
BK006947 Genomic DNA. Translation: DAA10446.1 .
PIRi S58253.
RefSeqi NP_014300.3. NM_001182937.3.

3D structure databases

ProteinModelPortali P50946.
SMRi P50946. Positions 67-220.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35724. 123 interactions.
DIPi DIP-1991N.
IntActi P50946. 4 interactions.
MINTi MINT-406344.
STRINGi 4932.YNL099C.

Proteomic databases

MaxQBi P50946.
PaxDbi P50946.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL099C ; YNL099C ; YNL099C .
GeneIDi 855624.
KEGGi sce:YNL099C.

Organism-specific databases

CYGDi YNL099c.
SGDi S000005043. OCA1.

Phylogenomic databases

eggNOGi COG2365.
GeneTreei ENSGT00530000066535.
HOGENOMi HOG000188365.
KOi K18043.
OMAi KLQRWNL.
OrthoDBi EOG7Z0K6V.

Enzyme and pathway databases

BioCyci YEAST:G3O-33127-MONOMER.

Miscellaneous databases

NextBioi 979822.

Gene expression databases

Genevestigatori P50946.

Family and domain databases

Gene3Di 3.90.190.10. 1 hit.
InterProi IPR029021. Prot-tyrosine_phosphatase-like.
IPR004861. Tyr_Pase_SIW14-like.
[Graphical view ]
Pfami PF03162. Y_phosphatase2. 1 hit.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Quantitative trait loci mapped to single-nucleotide resolution in yeast."
    Deutschbauer A.M., Davis R.W.
    Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT CYS-66.
    Strain: SK1.
  2. "The sequence of a 21.3 kb DNA fragment from the left arm of yeast chromosome XIV reveals LEU4, MET4, POL1, RAS2, and six new open reading frames."
    Saiz J.E., Buitrago M.J., Soler A., del Rey F., Revuelta J.L.
    Yeast 12:403-409(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Gathering STYX: phosphatase-like form predicts functions for unique protein-interaction domains."
    Wishart M.J., Dixon J.E.
    Trends Biochem. Sci. 23:301-306(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.
  6. "Identification of a Saccharomyces cerevisiae gene that is required for G1 arrest in response to the lipid oxidation product linoleic acid hydroperoxide."
    Alic N., Higgins V.J., Dawes I.W.
    Mol. Biol. Cell 12:1801-1810(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Lipid hydroperoxides activate the mitogen-activated protein kinase Mpk1p in Saccharomyces cerevisiae."
    Alic N., Higgins V.J., Pichova A., Breitenbach M., Dawes I.W.
    J. Biol. Chem. 278:41849-41855(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. Cited for: INTERACTION WITH OCA2; OCA4; OCA5 AND SIW14.
  11. "Oxidant-induced cell-cycle delay in Saccharomyces cerevisiae: the involvement of the SWI6 transcription factor."
    Fong C.S., Temple M.D., Alic N., Chiu J., Durchdewald M., Thorpe G.W., Higgins V.J., Dawes I.W.
    FEMS Yeast Res. 8:386-399(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiOCA1_YEAST
AccessioniPrimary (citable) accession number: P50946
Secondary accession number(s): D6W180, Q45U02
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2310 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

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