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Protein

Putative tyrosine-protein phosphatase OCA1

Gene

OCA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Putative tyrosine-protein phosphatase required for protection against superoxide stress. Involved in cell-cycle delay in response to linoleic acid hydroperoxide (LoaOOH).3 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei168 – 1681Phosphocysteine intermediateBy similarity

GO - Molecular functioni

  • protein tyrosine phosphatase activity Source: SGD

GO - Biological processi

  • cellular response to oxidative stress Source: SGD
  • peptidyl-tyrosine dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciYEAST:G3O-33127-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative tyrosine-protein phosphatase OCA1 (EC:3.1.3.48)
Alternative name(s):
Oxidant-induced cell-cycle arrest protein 1
Gene namesi
Name:OCA1
Ordered Locus Names:YNL099C
ORF Names:N2194
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIV

Organism-specific databases

CYGDiYNL099c.
EuPathDBiFungiDB:YNL099C.
SGDiS000005043. OCA1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 238237Putative tyrosine-protein phosphatase OCA1PRO_0000203439Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonine1 Publication
Modified residuei24 – 241Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP50946.
PaxDbiP50946.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
OCA2P539495EBI-28814,EBI-28725
SIW14P539654EBI-28814,EBI-28668

Protein-protein interaction databases

BioGridi35724. 122 interactions.
DIPiDIP-1991N.
IntActiP50946. 4 interactions.
MINTiMINT-406344.

Structurei

3D structure databases

ProteinModelPortaliP50946.
SMRiP50946. Positions 67-220.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2365.
GeneTreeiENSGT00530000066535.
HOGENOMiHOG000188365.
InParanoidiP50946.
KOiK18043.
OMAiNAWDPIT.
OrthoDBiEOG7Z0K6V.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR004861. Tyr_Pase_SIW14-like.
[Graphical view]
PfamiPF03162. Y_phosphatase2. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50946-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKVGEYED VPEDESRLTE ENVSVPEEEV EDEDEEEDDD DDHIYINEET
60 70 80 90 100
ESGREKVLVS HAPQERIVPP LNFCPVERYL YRSGQPSPVN FPFLLNLKLK
110 120 130 140 150
TIIWLSNEEP QDTLLEFCDT HRINLQFAAI NPDAGEDDNP WDGLTEHSII
160 170 180 190 200
NVLQTIVTQE NYPLLVCCGM GRHRTGTVIG CLRRIMGWNL ASVSEEYRRF
210 220 230
TGSRGGRILV ELLIEAFDTN LVKIDKNKAP SWLLTALE
Length:238
Mass (Da):27,205
Last modified:October 1, 1996 - v1
Checksum:i114DD87CBA2FAEBF
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti66 – 661R → C in strain: SK1. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ115393 Genomic DNA. Translation: AAZ22508.1.
Z50161 Genomic DNA. Translation: CAA90527.1.
Z71375 Genomic DNA. Translation: CAA95975.1.
BK006947 Genomic DNA. Translation: DAA10446.1.
PIRiS58253.
RefSeqiNP_014300.3. NM_001182937.3.

Genome annotation databases

EnsemblFungiiYNL099C; YNL099C; YNL099C.
GeneIDi855624.
KEGGisce:YNL099C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ115393 Genomic DNA. Translation: AAZ22508.1.
Z50161 Genomic DNA. Translation: CAA90527.1.
Z71375 Genomic DNA. Translation: CAA95975.1.
BK006947 Genomic DNA. Translation: DAA10446.1.
PIRiS58253.
RefSeqiNP_014300.3. NM_001182937.3.

3D structure databases

ProteinModelPortaliP50946.
SMRiP50946. Positions 67-220.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35724. 122 interactions.
DIPiDIP-1991N.
IntActiP50946. 4 interactions.
MINTiMINT-406344.

Proteomic databases

MaxQBiP50946.
PaxDbiP50946.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL099C; YNL099C; YNL099C.
GeneIDi855624.
KEGGisce:YNL099C.

Organism-specific databases

CYGDiYNL099c.
EuPathDBiFungiDB:YNL099C.
SGDiS000005043. OCA1.

Phylogenomic databases

eggNOGiCOG2365.
GeneTreeiENSGT00530000066535.
HOGENOMiHOG000188365.
InParanoidiP50946.
KOiK18043.
OMAiNAWDPIT.
OrthoDBiEOG7Z0K6V.

Enzyme and pathway databases

BioCyciYEAST:G3O-33127-MONOMER.

Miscellaneous databases

NextBioi979822.
PROiP50946.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR004861. Tyr_Pase_SIW14-like.
[Graphical view]
PfamiPF03162. Y_phosphatase2. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Quantitative trait loci mapped to single-nucleotide resolution in yeast."
    Deutschbauer A.M., Davis R.W.
    Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT CYS-66.
    Strain: SK1.
  2. "The sequence of a 21.3 kb DNA fragment from the left arm of yeast chromosome XIV reveals LEU4, MET4, POL1, RAS2, and six new open reading frames."
    Saiz J.E., Buitrago M.J., Soler A., del Rey F., Revuelta J.L.
    Yeast 12:403-409(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Gathering STYX: phosphatase-like form predicts functions for unique protein-interaction domains."
    Wishart M.J., Dixon J.E.
    Trends Biochem. Sci. 23:301-306(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.
  6. "Identification of a Saccharomyces cerevisiae gene that is required for G1 arrest in response to the lipid oxidation product linoleic acid hydroperoxide."
    Alic N., Higgins V.J., Dawes I.W.
    Mol. Biol. Cell 12:1801-1810(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Lipid hydroperoxides activate the mitogen-activated protein kinase Mpk1p in Saccharomyces cerevisiae."
    Alic N., Higgins V.J., Pichova A., Breitenbach M., Dawes I.W.
    J. Biol. Chem. 278:41849-41855(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. Cited for: INTERACTION WITH OCA2; OCA4; OCA5 AND SIW14.
  11. "Oxidant-induced cell-cycle delay in Saccharomyces cerevisiae: the involvement of the SWI6 transcription factor."
    Fong C.S., Temple M.D., Alic N., Chiu J., Durchdewald M., Thorpe G.W., Higgins V.J., Dawes I.W.
    FEMS Yeast Res. 8:386-399(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiOCA1_YEAST
AccessioniPrimary (citable) accession number: P50946
Secondary accession number(s): D6W180, Q45U02
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 22, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2310 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.