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P50945

- MIC27_YEAST

UniProt

P50945 - MIC27_YEAST

Protein

MICOS complex subunit MIC27

Gene

MIC27

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.2 Publications

    GO - Biological processi

    1. cristae formation Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33128-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    MICOS complex subunit MIC27
    Alternative name(s):
    Altered inheritance of mitochondria protein 37
    Mitochondrial contact site complex 27 kDa subunit
    Gene namesi
    Name:MIC27
    Synonyms:AIM37, MCS27
    Ordered Locus Names:YNL100W
    ORF Names:N2190
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL100w.
    SGDiS000005044. MIC27.

    Subcellular locationi

    Mitochondrion inner membrane Curated; Multi-pass membrane protein Curated
    Note: Enriched at crista junctions.5 Publications

    GO - Cellular componenti

    1. MICOS complex Source: SGD
    2. mitochondrial crista junction Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    Pathology & Biotechi

    Disruption phenotypei

    Increases frequency of mitochondrial genome loss. Partially altered shape of the mitochondrial network with condensed, fragmented mitochondria accumulating at the periphery of cells. 20-40% of mitochondria exhibit an increased inner membrane surface and stacks of lamellar cristae disconnected from the inner boundary membrane.3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 234234MICOS complex subunit MIC27PRO_0000203438Add
    BLAST

    Proteomic databases

    MaxQBiP50945.
    PaxDbiP50945.

    Expressioni

    Gene expression databases

    GenevestigatoriP50945.

    Interactioni

    Subunit structurei

    Component of the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MIC10, MIC12, MIC19, MIC26, MIC27 and MIC60. This complex was also known under the names MINOS or MitOS complex.1 Publication

    Protein-protein interaction databases

    BioGridi35723. 20 interactions.
    DIPiDIP-2042N.
    IntActiP50945. 9 interactions.
    MINTiMINT-565064.
    STRINGi4932.YNL100W.

    Structurei

    3D structure databases

    ProteinModelPortaliP50945.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 100100Mitochondrial intermembraneSequence AnalysisAdd
    BLAST
    Topological domaini121 – 14121Mitochondrial matrixSequence AnalysisAdd
    BLAST
    Topological domaini162 – 23473Mitochondrial intermembraneSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei101 – 12020HelicalSequence AnalysisAdd
    BLAST
    Transmembranei142 – 16120HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG40318.
    KOiK17789.
    OMAiNILTICA.
    OrthoDBiEOG7R83D8.

    Sequencei

    Sequence statusi: Complete.

    P50945-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVNFYDDVDE SKSHGEFPLI PVVLQNSSEL SVRTIPTGNE IIESVHLTKW    50
    LRKYRNALAS QLDRYEKGWQ SKIANFRLQV QHVINYSRKN IFNVDSENKH 100
    TVVPGSLIAL GAFFAGSIAV NRSNWGAKRL IFGHKSSILE KLCTSLPSRI 150
    LLPWVLAAAT FKYWAPQTSQ NLVNATENDL LPADFVKSYH NTWKRIYEEG 200
    YVAKKCDLKR QIDQTLQKNI RYAREQLYEK LEQA 234
    Length:234
    Mass (Da):26,960
    Last modified:October 1, 1996 - v1
    Checksum:i8DFD8FD42E71B8DB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ115393 Genomic DNA. Translation: AAZ22507.1.
    Z50161 Genomic DNA. Translation: CAA90526.1.
    Z71376 Genomic DNA. Translation: CAA95976.1.
    AY692704 Genomic DNA. Translation: AAT92723.1.
    BK006947 Genomic DNA. Translation: DAA10445.1.
    PIRiS58252.
    RefSeqiNP_014299.1. NM_001182938.1.

    Genome annotation databases

    EnsemblFungiiYNL100W; YNL100W; YNL100W.
    GeneIDi855623.
    KEGGisce:YNL100W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ115393 Genomic DNA. Translation: AAZ22507.1 .
    Z50161 Genomic DNA. Translation: CAA90526.1 .
    Z71376 Genomic DNA. Translation: CAA95976.1 .
    AY692704 Genomic DNA. Translation: AAT92723.1 .
    BK006947 Genomic DNA. Translation: DAA10445.1 .
    PIRi S58252.
    RefSeqi NP_014299.1. NM_001182938.1.

    3D structure databases

    ProteinModelPortali P50945.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35723. 20 interactions.
    DIPi DIP-2042N.
    IntActi P50945. 9 interactions.
    MINTi MINT-565064.
    STRINGi 4932.YNL100W.

    Proteomic databases

    MaxQBi P50945.
    PaxDbi P50945.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL100W ; YNL100W ; YNL100W .
    GeneIDi 855623.
    KEGGi sce:YNL100W.

    Organism-specific databases

    CYGDi YNL100w.
    SGDi S000005044. MIC27.

    Phylogenomic databases

    eggNOGi NOG40318.
    KOi K17789.
    OMAi NILTICA.
    OrthoDBi EOG7R83D8.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33128-MONOMER.

    Miscellaneous databases

    NextBioi 979819.

    Gene expression databases

    Genevestigatori P50945.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Quantitative trait loci mapped to single-nucleotide resolution in yeast."
      Deutschbauer A.M., Davis R.W.
      Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SK1.
    2. "The sequence of a 21.3 kb DNA fragment from the left arm of yeast chromosome XIV reveals LEU4, MET4, POL1, RAS2, and six new open reading frames."
      Saiz J.E., Buitrago M.J., Soler A., del Rey F., Revuelta J.L.
      Yeast 12:403-409(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "A scalable method for integration and functional analysis of multiple microarray datasets."
      Huttenhower C., Hibbs M., Myers C., Troyanskaya O.G.
      Bioinformatics 22:2890-2897(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PREDICTION OF FUNCTION.
    9. "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
      Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
      J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. "Computationally driven, quantitative experiments discover genes required for mitochondrial biogenesis."
      Hess D.C., Myers C.L., Huttenhower C., Hibbs M.A., Hayes A.P., Paw J., Clore J.J., Mendoza R.M., Luis B.S., Nislow C., Giaever G., Costanzo M., Troyanskaya O.G., Caudy A.A.
      PLoS Genet. 5:E1000407-E1000407(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    11. Cited for: FUNCTION, COMPOSITION OF THE MICOS COMPLEX, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    12. "The mitochondrial contact site complex, a determinant of mitochondrial architecture."
      Harner M., Korner C., Walther D., Mokranjac D., Kaesmacher J., Welsch U., Griffith J., Mann M., Reggiori F., Neupert W.
      EMBO J. 30:4356-4370(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE MICOS COMPLEX, MASS SPECTROMETRY, SUBCELLULAR LOCATION, TOPOLOGY.
    13. "A mitochondrial-focused genetic interaction map reveals a scaffold-like complex required for inner membrane organization in mitochondria."
      Hoppins S., Collins S.R., Cassidy-Stone A., Hummel E., Devay R.M., Lackner L.L., Westermann B., Schuldiner M., Weissman J.S., Nunnari J.
      J. Cell Biol. 195:323-340(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, COMPOSITION OF THE MICOS COMPLEX, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    14. Cited for: NOMENCLATURE.

    Entry informationi

    Entry nameiMIC27_YEAST
    AccessioniPrimary (citable) accession number: P50945
    Secondary accession number(s): D6W179, Q45U03
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3