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Protein

Vacuolar amino acid transporter 4

Gene

AVT4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in amino acid efflux from the vacuole to the cytoplasm. Capable of transporting large neutral amino acids including tyrosine, glutamine, asparagine, isoleucine and leucine.1 Publication

GO - Molecular functioni

  • L-glutamine transmembrane transporter activity Source: SGD
  • L-isoleucine transmembrane transporter activity Source: SGD
  • L-tyrosine transmembrane transporter activity Source: SGD

GO - Biological processi

  • amino acid transmembrane export from vacuole Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33129-MONOMER.
ReactomeiR-SCE-352230. Amino acid transport across the plasma membrane.
R-SCE-428559. Proton-coupled neutral amino acid transporters.

Protein family/group databases

TCDBi2.A.18.7.2. the amino acid/auxin permease (aaap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar amino acid transporter 4
Gene namesi
Name:AVT4
Ordered Locus Names:YNL101W
ORF Names:N2185
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL101W.
SGDiS000005045. AVT4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 242VacuolarSequence analysisAdd BLAST242
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 301CytoplasmicSequence analysisAdd BLAST38
Transmembranei302 – 322HelicalSequence analysisAdd BLAST21
Topological domaini323 – 326VacuolarSequence analysis4
Transmembranei327 – 347HelicalSequence analysisAdd BLAST21
Topological domaini348 – 373CytoplasmicSequence analysisAdd BLAST26
Transmembranei374 – 394HelicalSequence analysisAdd BLAST21
Topological domaini395 – 410VacuolarSequence analysisAdd BLAST16
Transmembranei411 – 431HelicalSequence analysisAdd BLAST21
Topological domaini432 – 438CytoplasmicSequence analysis7
Transmembranei439 – 459HelicalSequence analysisAdd BLAST21
Topological domaini460 – 483VacuolarSequence analysisAdd BLAST24
Transmembranei484 – 504HelicalSequence analysisAdd BLAST21
Topological domaini505 – 515CytoplasmicSequence analysisAdd BLAST11
Transmembranei516 – 536HelicalSequence analysisAdd BLAST21
Topological domaini537 – 561VacuolarSequence analysisAdd BLAST25
Transmembranei562 – 582HelicalSequence analysisAdd BLAST21
Topological domaini583 – 621CytoplasmicSequence analysisAdd BLAST39
Transmembranei622 – 642HelicalSequence analysisAdd BLAST21
Topological domaini643 – 648VacuolarSequence analysis6
Transmembranei649 – 669HelicalSequence analysisAdd BLAST21
Topological domaini670 – 692CytoplasmicSequence analysisAdd BLAST23
Transmembranei693 – 711HelicalSequence analysisAdd BLAST19
Topological domaini712 – 713VacuolarSequence analysis2

GO - Cellular componenti

  • fungal-type vacuole Source: SGD
  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • vacuole-mitochondrion membrane contact site Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000938371 – 713Vacuolar amino acid transporter 4Add BLAST713

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei88PhosphoserineCombined sources1
Modified residuei130PhosphoserineCombined sources1
Modified residuei165PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP50944.

PTM databases

iPTMnetiP50944.

Interactioni

Protein-protein interaction databases

BioGridi35722. 46 interactors.
DIPiDIP-6754N.
IntActiP50944. 2 interactors.
MINTiMINT-671686.

Structurei

3D structure databases

ProteinModelPortaliP50944.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000011245.
HOGENOMiHOG000185918.
InParanoidiP50944.
KOiK14209.
OMAiGHFGGEN.
OrthoDBiEOG092C13D8.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50944-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTNNGDGEH LGIRRNGNLR HPSNNMKIPR RAQSTVLNSN PFYSRKYSMS
60 70 80 90 100
TLTPRDICRS VDSRVFVDMS SPNFQTLEDP HRDEIINSVR LNYLNSSKRS
110 120 130 140 150
SVSHGNEAIP RVNPTKNSSA STIAAANVDS DDDETNLSSA GGDITHDIYK
160 170 180 190 200
LVKAEDPKRL RRPRSMENVT PKIEHHTKLS SASGLNVPGG FRREFIVNKK
210 220 230 240 250
RQEHQLNDSA SSDFTSHESD SINQSSPSSN QDIDKVPFLT RNFLEFLYVF
260 270 280 290 300
GHFAGESFED DFIPDSSNMM IRGEDERSAL LSRPDHMKVL PSAKGTTSTK
310 320 330 340 350
KVFLILLKSF IGTGVLFLPN AFHNGGLFFS VSMLAFFGIY SYWCYYILVQ
360 370 380 390 400
AKSSCGVSSF GDIGLKLYGP WMRIIILFSL VITQVGFSGA YMIFTAKNLQ
410 420 430 440 450
AFLDNVFHVG VLPLSYLMVF QTIIFIPLSF IRNISKLSLP SLLANFFIMA
460 470 480 490 500
GLVIVIIFTA KRLFFDLMGT PAMGVVYGLN ADRWTLFIGT AIFAFEGIGL
510 520 530 540 550
IIPVQDSMRN PEKFPLVLAL VILTATILFI SIATLGYLAY GSNVQTVILL
560 570 580 590 600
NLPQSNIFVN LIQLFYSIAI MLSTPLQLFP AIKIIENKFF PKFTKIYVKH
610 620 630 640 650
DDLTTRVELR PNSGKLNWKI KWLKNFIRSI IVIIVVSIAY FGSDNLDKFV
660 670 680 690 700
SVIGSLACIP LVYIYPSMLH LRGNSLPETK GEFWRFKPML DTILIFFGIA
710
SMLYTSYQSI FGV
Length:713
Mass (Da):80,026
Last modified:October 1, 1996 - v1
Checksum:iEAB458A2FEB660FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50161 Genomic DNA. Translation: CAA90525.1.
Z71377 Genomic DNA. Translation: CAA95977.1.
BK006947 Genomic DNA. Translation: DAA10444.1.
PIRiS58251.
RefSeqiNP_014298.1. NM_001182939.1.

Genome annotation databases

EnsemblFungiiYNL101W; YNL101W; YNL101W.
GeneIDi855622.
KEGGisce:YNL101W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50161 Genomic DNA. Translation: CAA90525.1.
Z71377 Genomic DNA. Translation: CAA95977.1.
BK006947 Genomic DNA. Translation: DAA10444.1.
PIRiS58251.
RefSeqiNP_014298.1. NM_001182939.1.

3D structure databases

ProteinModelPortaliP50944.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35722. 46 interactors.
DIPiDIP-6754N.
IntActiP50944. 2 interactors.
MINTiMINT-671686.

Protein family/group databases

TCDBi2.A.18.7.2. the amino acid/auxin permease (aaap) family.

PTM databases

iPTMnetiP50944.

Proteomic databases

PRIDEiP50944.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL101W; YNL101W; YNL101W.
GeneIDi855622.
KEGGisce:YNL101W.

Organism-specific databases

EuPathDBiFungiDB:YNL101W.
SGDiS000005045. AVT4.

Phylogenomic databases

GeneTreeiENSGT00390000011245.
HOGENOMiHOG000185918.
InParanoidiP50944.
KOiK14209.
OMAiGHFGGEN.
OrthoDBiEOG092C13D8.

Enzyme and pathway databases

BioCyciYEAST:G3O-33129-MONOMER.
ReactomeiR-SCE-352230. Amino acid transport across the plasma membrane.
R-SCE-428559. Proton-coupled neutral amino acid transporters.

Miscellaneous databases

PROiP50944.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAVT4_YEAST
AccessioniPrimary (citable) accession number: P50944
Secondary accession number(s): D6W178
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.