Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei85SubstrateBy similarity1
Binding sitei117NAD or substrateBy similarity1
Binding sitei146SubstrateBy similarity1
Active sitei170Proton acceptorBy similarity1
Binding sitei225SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 37NADBy similarityAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.1.1.27. 1856.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase (EC:1.1.1.27)
Short name:
L-LDH
Gene namesi
Name:ldh
Ordered Locus Names:DR_2364
OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Taxonomic identifieri243230 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
Proteomesi
  • UP000002524 Componenti: Chromosome I

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001683411 – 304L-lactate dehydrogenaseAdd BLAST304

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi243230.DR_2364.

Structurei

Secondary structure

1304
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi10 – 21Combined sources12
Beta strandi26 – 31Combined sources6
Helixi35 – 45Combined sources11
Beta strandi56 – 60Combined sources5
Helixi62 – 65Combined sources4
Beta strandi69 – 73Combined sources5
Helixi89 – 106Combined sources18
Beta strandi108 – 114Combined sources7
Beta strandi116 – 118Combined sources3
Helixi119 – 129Combined sources11
Beta strandi135 – 137Combined sources3
Helixi141 – 155Combined sources15
Helixi159 – 161Combined sources3
Beta strandi166 – 171Combined sources6
Beta strandi174 – 176Combined sources3
Helixi178 – 180Combined sources3
Helixi188 – 195Combined sources8
Helixi201 – 211Combined sources11
Helixi227 – 241Combined sources15
Beta strandi246 – 253Combined sources8
Turni255 – 257Combined sources3
Beta strandi258 – 268Combined sources11
Beta strandi271 – 275Combined sources5
Helixi282 – 296Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V6BX-ray2.50A/B/C/D1-304[»]
ProteinModelPortaliP50933.
SMRiP50933.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50933.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213793.
InParanoidiP50933.
KOiK00016.
OMAiNNEHRVL.
OrthoDBiPOG091H02EQ.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50933-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVGVVGTGF VGSTAAFALV LRGSCSELVL VDRDEDRAQA EAEDIAHAAP
60 70 80 90 100
VSHGTRVWHG GHSELADAQV VILTAGANQK PGESRLDLLE KNADIFRELV
110 120 130 140 150
PQITRAAPDA VLLVTSNPVD LLTDLATQLA PGQPVIGSGT VLDSARFRHL
160 170 180 190 200
MAQHAGVDGT HAHGYVLGEH GDSEVLAWSS AMVAGMPVAD FMQAQNLPWN
210 220 230 240 250
EQVRAKIDEG TRNAAASIIE GKRATYYGIG AALARITEAV LRDRRAVLTV
260 270 280 290 300
SAPTPEYGVS LSLPRVVGRQ GVLSTLHPKL TGDEQQKLEQ SAGVLRGFKQ

QLGL
Length:304
Mass (Da):32,164
Last modified:May 30, 2000 - v2
Checksum:iF4CF3298673010CD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti270Q → R in BAA21471 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005539 Genomic DNA. Translation: BAA21471.1.
AE000513 Genomic DNA. Translation: AAF11912.1.
PIRiE75282.
RefSeqiNP_296085.1. NC_001263.1.
WP_010888990.1. NZ_CP015081.1.

Genome annotation databases

EnsemblBacteriaiAAF11912; AAF11912; DR_2364.
GeneIDi1799712.
KEGGidra:DR_2364.
PATRICi21632442. VBIDeiRad64572_2598.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005539 Genomic DNA. Translation: BAA21471.1.
AE000513 Genomic DNA. Translation: AAF11912.1.
PIRiE75282.
RefSeqiNP_296085.1. NC_001263.1.
WP_010888990.1. NZ_CP015081.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V6BX-ray2.50A/B/C/D1-304[»]
ProteinModelPortaliP50933.
SMRiP50933.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243230.DR_2364.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF11912; AAF11912; DR_2364.
GeneIDi1799712.
KEGGidra:DR_2364.
PATRICi21632442. VBIDeiRad64572_2598.

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213793.
InParanoidiP50933.
KOiK00016.
OMAiNNEHRVL.
OrthoDBiPOG091H02EQ.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
BRENDAi1.1.1.27. 1856.

Miscellaneous databases

EvolutionaryTraceiP50933.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDH_DEIRA
AccessioniPrimary (citable) accession number: P50933
Secondary accession number(s): O32512
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 30, 2000
Last modified: November 2, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.