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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851SubstrateBy similarity
Binding sitei117 – 1171NAD or substrateBy similarity
Binding sitei146 – 1461SubstrateBy similarity
Active sitei170 – 1701Proton acceptorBy similarity
Binding sitei225 – 2251SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 3729NADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciDRAD243230:GH46-2413-MONOMER.
BRENDAi1.1.1.27. 1856.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase (EC:1.1.1.27)
Short name:
L-LDH
Gene namesi
Name:ldh
Ordered Locus Names:DR_2364
OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Taxonomic identifieri243230 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
Proteomesi
  • UP000002524 Componenti: Chromosome I

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304L-lactate dehydrogenasePRO_0000168341Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi243230.DR_2364.

Structurei

Secondary structure

1
304
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65Combined sources
Helixi10 – 2112Combined sources
Beta strandi26 – 316Combined sources
Helixi35 – 4511Combined sources
Beta strandi56 – 605Combined sources
Helixi62 – 654Combined sources
Beta strandi69 – 735Combined sources
Helixi89 – 10618Combined sources
Beta strandi108 – 1147Combined sources
Beta strandi116 – 1183Combined sources
Helixi119 – 12911Combined sources
Beta strandi135 – 1373Combined sources
Helixi141 – 15515Combined sources
Helixi159 – 1613Combined sources
Beta strandi166 – 1716Combined sources
Beta strandi174 – 1763Combined sources
Helixi178 – 1803Combined sources
Helixi188 – 1958Combined sources
Helixi201 – 21111Combined sources
Helixi227 – 24115Combined sources
Beta strandi246 – 2538Combined sources
Turni255 – 2573Combined sources
Beta strandi258 – 26811Combined sources
Beta strandi271 – 2755Combined sources
Helixi282 – 29615Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V6BX-ray2.50A/B/C/D1-304[»]
ProteinModelPortaliP50933.
SMRiP50933. Positions 13-299.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50933.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213793.
InParanoidiP50933.
KOiK00016.
OMAiAGDYEDC.
OrthoDBiEOG6091FG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50933-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVGVVGTGF VGSTAAFALV LRGSCSELVL VDRDEDRAQA EAEDIAHAAP
60 70 80 90 100
VSHGTRVWHG GHSELADAQV VILTAGANQK PGESRLDLLE KNADIFRELV
110 120 130 140 150
PQITRAAPDA VLLVTSNPVD LLTDLATQLA PGQPVIGSGT VLDSARFRHL
160 170 180 190 200
MAQHAGVDGT HAHGYVLGEH GDSEVLAWSS AMVAGMPVAD FMQAQNLPWN
210 220 230 240 250
EQVRAKIDEG TRNAAASIIE GKRATYYGIG AALARITEAV LRDRRAVLTV
260 270 280 290 300
SAPTPEYGVS LSLPRVVGRQ GVLSTLHPKL TGDEQQKLEQ SAGVLRGFKQ

QLGL
Length:304
Mass (Da):32,164
Last modified:May 30, 2000 - v2
Checksum:iF4CF3298673010CD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti270 – 2701Q → R in BAA21471 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005539 Genomic DNA. Translation: BAA21471.1.
AE000513 Genomic DNA. Translation: AAF11912.1.
PIRiE75282.
RefSeqiNP_296085.1. NC_001263.1.
WP_010888990.1. NC_001263.1.

Genome annotation databases

EnsemblBacteriaiAAF11912; AAF11912; DR_2364.
GeneIDi1799712.
KEGGidra:DR_2364.
PATRICi21632442. VBIDeiRad64572_2598.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005539 Genomic DNA. Translation: BAA21471.1.
AE000513 Genomic DNA. Translation: AAF11912.1.
PIRiE75282.
RefSeqiNP_296085.1. NC_001263.1.
WP_010888990.1. NC_001263.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V6BX-ray2.50A/B/C/D1-304[»]
ProteinModelPortaliP50933.
SMRiP50933. Positions 13-299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243230.DR_2364.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF11912; AAF11912; DR_2364.
GeneIDi1799712.
KEGGidra:DR_2364.
PATRICi21632442. VBIDeiRad64572_2598.

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213793.
InParanoidiP50933.
KOiK00016.
OMAiAGDYEDC.
OrthoDBiEOG6091FG.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
BioCyciDRAD243230:GH46-2413-MONOMER.
BRENDAi1.1.1.27. 1856.

Miscellaneous databases

EvolutionaryTraceiP50933.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the L-lactate dehydrogenase-encoding gene of Deinococcus radiodurans, a suitable mesophilic counterpart for Thermus."
    Narumi I., Watanabe H.
    Gene 172:117-119(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.
  2. "Revised nucleotide sequence of the Deinococcus radiodurans L-lactate dehydrogenase gene and the gene expression in Escherichia coli."
    Narumi I., Watanabe H.
    Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 270 AND 277.
    Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.

Entry informationi

Entry nameiLDH_DEIRA
AccessioniPrimary (citable) accession number: P50933
Secondary accession number(s): O32512
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 30, 2000
Last modified: December 9, 2015
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.